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Literature summary extracted from
- Stimulation of p-nitrophenylphosphatase activity of Na+/K+-ATPase by NaCl with oligomycin or ATP (2005), FEBS J., 272, 673-684.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.41 | ATP | activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase only in presence of NaCl, cannot be substituted by nonhydrolyzable ATP analogues for stimulation | Canis lupus familiaris |  |
| 3.1.3.41 | additional information | stimulation mechanism and kinetics | Canis lupus familiaris | |
| 3.1.3.41 | oligomycin | activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase only in presence of NaCl | Canis lupus familiaris | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.3.41 | microsome | - |
Canis lupus familiaris | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.41 | KCl | activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase only in presence of NaCl, gradually inhibits ATPase activity of the enzyme | Canis lupus familiaris | |
| 3.1.3.41 | Mg2+ | - |
Canis lupus familiaris | |
| 3.1.3.41 | NaCl | activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase, increases stimulation by other agents, e.g. KCl, oligomycin, and ATP, stimulation mechanism and kinetics, is also required for ATPase activity of the enzyme | Canis lupus familiaris | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.41 | 4-nitrophenyl phosphate + H2O | Canis lupus familiaris | - |
4-nitrophenol + phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.41 | Canis lupus familiaris | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.41 | partially by microsome preperation | Canis lupus familiaris |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.3.41 | kidney | medulla | Canis lupus familiaris | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.41 | 0.002 | - |
4-nitrophenyl phosphatase activity of Na+/K+-ATPase in microsomes | Canis lupus familiaris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.41 | 4-nitrophenyl phosphate + H2O | - |
Canis lupus familiaris | 4-nitrophenol + phosphate | - |
? | |
| 3.1.3.41 | additional information | the canine Na+/K+-ATPase shows 4-nitrophenyl phosphatase activity | Canis lupus familiaris | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.41 | p-nitrophenylphosphatase | - |
Canis lupus familiaris |
| 3.1.3.41 | PNPPase | - |
Canis lupus familiaris |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.41 | 23 | - |
assay at | Canis lupus familiaris |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.41 | 6.4 | 8.4 | assay at pH 6.4, pH 7.4, and at pH 8.4 | Canis lupus familiaris |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.41 | 6.4 | 8.4 | - |
Canis lupus familiaris |
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