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Literature summary extracted from
- Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana (2005), FEBS J., 272, 1291-1304.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.4.1.15 | expression in Escherichia coli | Arabidopsis thaliana |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.4.1.15 | glycerol, pyridoxal-5'-phosphate, dithiothreitol and EDTA do not increase the stability of the protein after freezing | Arabidopsis thaliana |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.15 | aminooxy acetic acid | at 10 microM, IC50 value is 0.0305 mM | Arabidopsis thaliana |  |
| 4.4.1.15 | hydroxylamine | at 10 microM, IC50 value is 0.0159 mM | Arabidopsis thaliana | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.15 | 0.25 | - |
D-cysteine | purified recombinant mature protein | Arabidopsis thaliana | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.4.1.15 | mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.15 | 41700 | - |
monomer, mature protein without N-terminal extension, calculated from amino acid sequence | Arabidopsis thaliana |
| 4.4.1.15 | 42000 | - |
x * 42000, SDS-PAGE, unprocessed protein | Arabidopsis thaliana |
| 4.4.1.15 | 43000 | - |
x * 43000, SDS-PAGE, mature protein | Arabidopsis thaliana |
| 4.4.1.15 | 43900 | - |
monomer, calculated from amino acid sequence | Arabidopsis thaliana |
| 4.4.1.15 | 44000 | - |
x * 44000, SDS-PAGE, post-translationally modified protein | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.15 | D-cysteine + H2O | Arabidopsis thaliana | - |
sulfide + NH3 + pyruvate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.4.1.15 | Arabidopsis thaliana | - |
ecotype C24 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.4.1.15 | recombinant protein | Arabidopsis thaliana |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.15 | D-cysteine + H2O | - |
Arabidopsis thaliana | sulfide + NH3 + pyruvate | - |
? | |
| 4.4.1.15 | D-cysteine + H2O | 37°C, pH 8.0 | Arabidopsis thaliana | sulfide + NH3 + pyruvate | - |
? | |
| 4.4.1.15 | L-cysteine + H2O | 5% activity compared to D-cysteine | Arabidopsis thaliana | sulfide + NH3 + pyruvate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.4.1.15 | ? | x * 42000, SDS-PAGE, unprocessed protein | Arabidopsis thaliana |
| 4.4.1.15 | ? | x * 43000, SDS-PAGE, mature protein | Arabidopsis thaliana |
| 4.4.1.15 | ? | x * 44000, SDS-PAGE, post-translationally modified protein | Arabidopsis thaliana |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.4.1.15 | D-CDes | - |
Arabidopsis thaliana |
| 4.4.1.15 | D-cysteine desulfhydrase | - |
Arabidopsis thaliana |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.15 | additional information | - |
loss of activity at 50°C, no activity at 60°C. The enzyme is also very sensitive to freezing. One freeze-thaw cycle leads to a loss of activity of 75%. | Arabidopsis thaliana |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.15 | 6 | - |
D-cysteine | - |
Arabidopsis thaliana | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.15 | 8 | - |
- |
Arabidopsis thaliana |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.15 | pyridoxal 5'-phosphate | - |
Arabidopsis thaliana | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 4.4.1.15 | Arabidopsis thaliana | mature protein without N-terminal extension, calculated from amino acid sequence | - |
4.34 |
| 4.4.1.15 | Arabidopsis thaliana | calculated from amino acid sequence | - |
7.2 |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.15 | 0.0159 | - |
at 10 microM, IC50 value is 0.0159 mM | Arabidopsis thaliana | hydroxylamine | |
| 4.4.1.15 | 0.0305 | - |
at 10 microM, IC50 value is 0.0305 mM | Arabidopsis thaliana | aminooxy acetic acid | |
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