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Literature summary extracted from

  • Byrnes, W.M.; Vilker, V.L.
    Extrinsic factors potassium chloride and glycerol induce thermostability in recombinant anthranilate synthase from Archaeoglobus fulgidus (2004), Extremophiles, 8, 455-462.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.27 expression in Escherichia coli Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.27 L-Trp competitive with respect to chorismate Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.27 0.0123
-
 chorismate pH 8.0, 60°C, without glycerol and KCl Archaeoglobus fulgidus
4.1.3.27 0.084
-
 chorismate pH 8.0, 60°C, with 25% glycerol and 2 M KCl Archaeoglobus fulgidus
4.1.3.27 5.7
-
 NH4+ pH 8.0, 60°C, without glycerol and KCl Archaeoglobus fulgidus
4.1.3.27 6.2
-
 L-Gln pH 8.0, 60°C, without glycerol and KCl Archaeoglobus fulgidus
4.1.3.27 7
-
 L-Gln pH 8.0, 60°C, with 25% glycerol and 2 M KCl Archaeoglobus fulgidus
4.1.3.27 7.3
-
 NH4+ pH 8.0, 60°C, with 25% glycerol and 2 M KCl Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.27 18000
-
 alpha2beta2, 2 * 18000 + 2 * 50000, SDS-PAGE Archaeoglobus fulgidus
4.1.3.27 50000
-
 alpha2beta2, 2 * 18000 + 2 * 50000, SDS-PAGE Archaeoglobus fulgidus
4.1.3.27 150000
-
 gel filtration Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.27 Archaeoglobus fulgidus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.27
-
 Archaeoglobus fulgidus

Storage Stability

EC Number Storage Stability Organism
4.1.3.27 -20°C, in a 1:1 mixture of glycerol, 50 mM tricine, pH 7.5,1 mM DTT, 0.1 mM EDTA, additional protein bands appear upon storage, after an initial 10 –15% drop in activity, little or no loss of enzyme activity is observed over a period of several weeks Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.27 chorismate + L-Gln
-
 Archaeoglobus fulgidus anthranilate + pyruvate + L-glutamate
-
 ?
4.1.3.27 chorismate + NH4+
-
 Archaeoglobus fulgidus anthranilate + pyruvate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.27 tetramer alpha2beta2, 2 * 18000 + 2 * 50000, SDS-PAGE Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.27 75
-
 half-life in presence of 25% glycerol without KCl: 4.6 min, in 25% glycerol with 2 M KCl activity is constant for over 4 h after initial drop in activity to 83% Archaeoglobus fulgidus
4.1.3.27 80
-
 half-life in presence of 25% glycerol without KCl: 1.2 min, half-life in presence of 25% glycerol and 2 M KCl: 200 min Archaeoglobus fulgidus
4.1.3.27 85
-
 half-life in presence of 25% glycerol without KCl: 0.9 min, half-life in presence of 25% glycerol and 2 M KCl: 50 min Archaeoglobus fulgidus
4.1.3.27 90
-
 half-life in presence of 25% glycerol and 2 M KCl: 12 min Archaeoglobus fulgidus
4.1.3.27 95
-
 half-life in presence of 25% glycerol and 2 M KCl: 4.1 min Archaeoglobus fulgidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.27 0.25
-
 chorismate pH 8.0, 60°C, with or without 2 M KCl and 25% glycerol Archaeoglobus fulgidus
4.1.3.27 0.25
-
 L-Gln pH 8.0, 60°C, with or without 2 M KCl and 25% glycerol Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.27 8
-
 NH4+-dependent reaction, with 25% glycerol and 2 M KCl Archaeoglobus fulgidus
4.1.3.27 8.1
-
 L-Gln-dependent reaction, with 25% glycerol and 2 M KCl Archaeoglobus fulgidus
4.1.3.27 8.7
-
 NH4+-dependent reaction, without glycerol and KCl Archaeoglobus fulgidus
4.1.3.27 8.8
-
 L-Gln+-dependent reaction, without glycerol and KCl Archaeoglobus fulgidus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.3.27 0.00028
-
 L-Trp pH 8.0, 60°C, without glycerol and KCl Archaeoglobus fulgidus
4.1.3.27 0.00031
-
 L-Trp pH 8.0, 60°C, with 25% glycerol and 2 M KCl Archaeoglobus fulgidus