BRENDA - Enzyme Database

Expression in yeast of a novel phospholipase A1 cDNA from Arabidopsis thaliana

Noiriel, A.; Benveniste, P.; Banas, A.; Stymne, S.; Bouvier-Nave, P.; Eur. J. Biochem. 271, 3752-3764 (2004)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.1.32
D384A
less than 10% of wild-type activity
Arabidopsis thaliana
3.1.1.32
H409L
less than 10% of wild-type activity
Arabidopsis thaliana
3.1.1.32
S177A
less than 10% of wild-type activity
Arabidopsis thaliana
3.1.1.32
T352A
about 70% of wild-type activity
Arabidopsis thaliana
3.1.1.32
Y346F
about 30% of wild-type activity
Arabidopsis thaliana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.32
microsome
associated with microsomal membrane
Arabidopsis thaliana
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.32
additional information
independent of Ca2+
Arabidopsis thaliana
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.32
Arabidopsis thaliana
Q93V61
expression in Saccharomyces cerevisiae
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.32
1-myristoyl 2-oleyl phosphatidylcholine + H2O
-
664829
Arabidopsis thaliana
2-oleylglycerophosphocholine + myristoate
-
-
-
-
3.1.1.32
lysophosphatidylcholine + H2O
weak substrate
664829
Arabidopsis thaliana
?
-
-
-
-
3.1.1.32
additional information
no substrate: tripalmitoylglycerol, cholesteryl oleate. Enzyme is sn-1 specific
664829
Arabidopsis thaliana
?
-
-
-
-
3.1.1.32
phosphatidic acid + H2O
-
664829
Arabidopsis thaliana
2-acylglycerol-3-phosphate + a carboxylate
-
-
-
?
3.1.1.32
phosphatidylethanolamine + H2O
-
664829
Arabidopsis thaliana
2-acylglycerophosphoethanolamine + a carboxylate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.32
60
65
-
Arabidopsis thaliana
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.32
6
6.5
-
Arabidopsis thaliana
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.1.32
D384A
less than 10% of wild-type activity
Arabidopsis thaliana
3.1.1.32
H409L
less than 10% of wild-type activity
Arabidopsis thaliana
3.1.1.32
S177A
less than 10% of wild-type activity
Arabidopsis thaliana
3.1.1.32
T352A
about 70% of wild-type activity
Arabidopsis thaliana
3.1.1.32
Y346F
about 30% of wild-type activity
Arabidopsis thaliana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.32
microsome
associated with microsomal membrane
Arabidopsis thaliana
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.32
additional information
independent of Ca2+
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.32
1-myristoyl 2-oleyl phosphatidylcholine + H2O
-
664829
Arabidopsis thaliana
2-oleylglycerophosphocholine + myristoate
-
-
-
-
3.1.1.32
lysophosphatidylcholine + H2O
weak substrate
664829
Arabidopsis thaliana
?
-
-
-
-
3.1.1.32
additional information
no substrate: tripalmitoylglycerol, cholesteryl oleate. Enzyme is sn-1 specific
664829
Arabidopsis thaliana
?
-
-
-
-
3.1.1.32
phosphatidic acid + H2O
-
664829
Arabidopsis thaliana
2-acylglycerol-3-phosphate + a carboxylate
-
-
-
?
3.1.1.32
phosphatidylethanolamine + H2O
-
664829
Arabidopsis thaliana
2-acylglycerophosphoethanolamine + a carboxylate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.32
60
65
-
Arabidopsis thaliana
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.32
6
6.5
-
Arabidopsis thaliana