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Literature summary extracted from

  • Gagyi, C.; Bucurenci, N.; Sirbu, O.; Labesse, G.; Ionescu, M.; Ofiteru, A.; Assairi, L.; Landais, S.; Danchin, A.; Barzu, O.; Gilles, A.M.
    UMP kinase from the Gram-positive bacterium Bacillus subtilis is strongly dependent on GTP for optimal activity (2003), Eur. J. Biochem., 270, 3196-3204.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.22 3'-anthraniloyl-2'-deoxyguanosine-5'-triphosphate
-
 Bacillus subtilis
2.7.4.22 dGTP
-
 Bacillus subtilis
2.7.4.22 GTP half-maximum activation is reached at 0.1 mM GTP, in the absence of GTP the enzyme exhibits less than 10% of its maximum activity Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.22
-
 Escherichia coli
2.7.4.22 expression in Escherichia coli strain BL21(DE3)/pDIA17 Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.22 UTP
-
 Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.22 0.008
-
 UMP in the absence of GTP Bacillus subtilis
2.7.4.22 0.03
-
 UMP in the presence of GTP Bacillus subtilis
2.7.4.22 0.05
-
 UMP
-
 Escherichia coli
2.7.4.22 0.11
-
 5-fluoro-UMP
-
 Escherichia coli
2.7.4.22 0.12
-
 5-fluoro-UMP
-
 Bacillus subtilis
2.7.4.22 0.14
-
 6-aza-UMP
-
 Bacillus subtilis
2.7.4.22 0.71
-
 6-aza-UMP
-
 Escherichia coli
2.7.4.22 0.9
-
 ATP
-
 Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.22 26080
-
 monomer, calculated from amino acid sequence Bacillus subtilis
2.7.4.22 26080
-
 monomer, mass spectroscopy Bacillus subtilis
2.7.4.22 28120
-
 His-tagged protein, monomer, mass spectroscopy Bacillus subtilis
2.7.4.22 28250
-
 His-tagged protein, monomer, calculated from amino acid sequence Bacillus subtilis
2.7.4.22 154000
-
 gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.22 ATP + UMP Escherichia coli
-
 ADP + UDP
-
 ?
2.7.4.22 ATP + UMP Bacillus subtilis
-
 ADP + UDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.22 Bacillus subtilis O31749
-
-
2.7.4.22 Escherichia coli P0A7E9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.22
-
 Escherichia coli
2.7.4.22
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.22 0.6
-
 with 6-aza-UMP as substrate Bacillus subtilis
2.7.4.22 24
-
 with 5-fluoro-UMP as substrate Bacillus subtilis
2.7.4.22 25
-
 His-tagged protein Bacillus subtilis
2.7.4.22 26
-
 native protein Bacillus subtilis
2.7.4.22 67
-
 with 6-aza-UMP as substrate Escherichia coli
2.7.4.22 126
-
 with UMP as substrate Escherichia coli
2.7.4.22 162
-
 with 5-fluoro-UMP as substrate Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.4.22 room temperature, 50 mM Tris-HCl (pH 7.4), 0.1 M NaCl, 2 mM UTP, 2 weeks, no loss of activity Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.22 ATP + 5-fluoro-UMP
-
 Escherichia coli ADP + 5-fluoro-UDP
-
 ?
2.7.4.22 ATP + 5-fluoro-UMP
-
 Bacillus subtilis ADP + 5-fluoro-UDP
-
 ?
2.7.4.22 ATP + 6-aza-UMP
-
 Escherichia coli ADP + 6-aza-UDP
-
 ?
2.7.4.22 ATP + 6-aza-UMP
-
 Bacillus subtilis ADP + 6-aza-UDP
-
 ?
2.7.4.22 ATP + UMP
-
 Escherichia coli ADP + UDP
-
 ?
2.7.4.22 ATP + UMP
-
 Bacillus subtilis ADP + UDP
-
 ?
2.7.4.22 ATP + UMP pH 7.4, 2 mM MgCl2, 30°C Escherichia coli ADP + UDP
-
 ?
2.7.4.22 ATP + UMP pH 7.4, 2 mM MgCl2, 30°C Bacillus subtilis ADP + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.22 hexamer alpha6, gel filtration Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
2.7.4.22 UMP kinase
-
 Escherichia coli
2.7.4.22 UMP kinase
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.22 42
-
 half-maximal inactivation Bacillus subtilis
2.7.4.22 70
-
 half-maximal inactivation in the presence of 1 mM UTP Bacillus subtilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.22 0.022
-
 UTP
-
 Bacillus subtilis