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Literature summary extracted from

  • Massimelli, M.J.; Beassoni, P.R.; Forrellad, M.A.; Barra, J.L.; Garrido, M.N.; Domenech, C.E.; Lisa, A.T.
    Identification, cloning, and expression of Pseudomonas aeruginosa phosphorylcholine phosphatase gene (2005), Curr. Microbiol., 50, 251-256.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.75 additional information the enzyme is induced by growth on choline, betaine, dimethylglycine, or carnitine, choline and betaine are inhibitory at high concentrations Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.75 gene PA5292, expression in Escherichia coli, expression in and complementation of a Pseudomonas aeruginosa mutant strain JUF8-00 Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.75 additional information Tn5-751 transposon mutagenesis is used to construct an enzyme-deficient, inactive mutant JUF8-00 of Pseudomonas aeruginosa, the mutant can be complemented by expression of the wild-type enzyme Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.75 betaine 32% inhibition of wild-type enzyme in vivo at 5 mM, at lower concentrations betaine induces the enzyme expression in vivo Pseudomonas aeruginosa
3.1.3.75 choline 12% inhibition of wild-type enzyme in vivo at 5 mM, at lower concentrations choline induces the enzyme expression in vivo Pseudomonas aeruginosa
3.1.3.75 additional information no inhibition by high concentrations of phosphorylcholine Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.75 periplasm
-
 Pseudomonas aeruginosa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.75 Mg2+
-
 Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.75 O-phosphocholine + H2O Pseudomonas aeruginosa
-
 choline + phosphate
-
 ?
3.1.3.75 O-phosphoethanolamine + H2O Pseudomonas aeruginosa
-
 ethanolamine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.75 Pseudomonas aeruginosa
-
 gene PA5292
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.75 recombinant enzyme from Escherichia coli by chitin affinity chromatography Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.75 additional information
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.75 4-nitrophenyl phosphate + H2O
-
 Pseudomonas aeruginosa 4-nitrophenol + phosphate
-
 ?
3.1.3.75 O-phosphocholine + H2O
-
 Pseudomonas aeruginosa choline + phosphate
-
 ?
3.1.3.75 O-phosphoethanolamine + H2O
-
 Pseudomonas aeruginosa ethanolamine + phosphate
-
 ?
3.1.3.75 phospho-L-serine + H2O
-
 Pseudomonas aeruginosa L-serine + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.75 PChP
-
 Pseudomonas aeruginosa
3.1.3.75 phosphorylcholine phosphatase
-
 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.75 5
-
 assay at, substrates phospho-L-serine and 4-nitrophenyl phosphate Pseudomonas aeruginosa
3.1.3.75 7.4
-
 assay at, substrates O-phosphocholine and phospho-L-serine Pseudomonas aeruginosa