EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.13.9 | glycine | activation of activity against substrates L-Pro-L-Ala, L-Pro-L-Val, L-Pro-L-Met, and L-Pro-L-Asp | Homo sapiens | |
3.4.13.9 | MnCl2 | stimulation of activity against substrates L-Pro-Gly, L-Pro-L-Glu, L-Pro-L-Leu, L-Pro-L-Ser, and L-Pro-L-Phe, inhibitory to hydrolysis of substrates L-Pro-L-Ala, L-Pro-L-Val, L-Pro-L-Met, and L-Pro-L-Asp | Homo sapiens | |
3.4.13.18 | Ala | prolinase activity in erythrocytes from both the normal control and the patients with prolidase (EC 3.4.13.9) deficiency is activated | Homo sapiens | |
3.4.13.18 | D-Leu | enhances activity at concentrations below 20 mM | Homo sapiens | |
3.4.13.18 | D-Val | enhances activity at concentrations below 50 mM | Homo sapiens | |
3.4.13.18 | Gly | prolinase activity in erythrocytes from both the normal control and the patients with prolidase (EC 3.4.13.9) deficiency is activated | Homo sapiens | |
3.4.13.18 | Ser | prolinase activity in erythrocytes from both the normal control and the patients with prolidase (EC 3.4.13.9) deficiency is activated | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.13.18 | L-leucine | strong inhibition of prolinase activity against Pro-Gly regardless wether MnCl2 is present or not | Homo sapiens | |
3.4.13.18 | L-Val | strong inhibition of prolinase activity against Pro-Gly regardless wether MnCl2 is present or not | Homo sapiens | |
3.4.13.18 | Mn2+ | 0.1 mM, inhibits prolinase activity in normal erythrocytes against Pro-Ala, Pro-Val, Pro-Met, Pro-Asp | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.13.9 | 3.2 | - |
L-Pro-L-Val | pH 7.8, 37°C, presence of 20 mM glycine, enzyme from patient with enzyme deficiency | Homo sapiens | |
3.4.13.9 | 4.2 | - |
L-Pro-L-Met | pH 7.8, 37°C, presence of 20 mM glycine, enzyme from patient with enzyme deficiency | Homo sapiens | |
3.4.13.9 | 5 | - |
L-Pro-L-Met | pH 7.8, 37°C, presence of 0.1 mM MnCl2 plus 20 mM glycine, enzyme from patient with enzyme deficiency | Homo sapiens | |
3.4.13.9 | 5.5 | - |
L-Pro-L-Val | pH 7.8, 37°C, presence of 0.1 mM MnCl2 plus 20 mM glycine, enzyme from patient with enzyme deficiency | Homo sapiens | |
3.4.13.9 | 6.8 | - |
L-Pro-Gly | pH 7.8, 37°C, presence of 0.1 mM MnCl2 plus 20 mM glycine | Homo sapiens | |
3.4.13.9 | 7.9 | - |
L-Pro-L-Val | pH 7.8, 37°C, enzyme from patient with enzyme deficiency | Homo sapiens | |
3.4.13.9 | 8.1 | - |
L-Pro-L-Met | pH 7.8, 37°C, enzyme from patient with enzyme deficiency | Homo sapiens | |
3.4.13.9 | 8.35 | - |
L-Pro-L-Met | pH 7.8, 37°C, presence of 20 mM glycine | Homo sapiens | |
3.4.13.9 | 8.6 | - |
L-Pro-Gly | pH 7.8, 37°C, presence of 0.1 mM MnCl2 plus 20 mM glycine, enzyme from patient with enzyme deficiency | Homo sapiens | |
3.4.13.9 | 9.7 | - |
L-Pro-Gly | pH 7.8, 37°C, presence of 0.1 mM MnCl2, enzyme from patient with enzyme deficiency | Homo sapiens | |
3.4.13.9 | 9.7 | - |
L-Pro-L-Met | pH 7.8, 37°C, presence of 0.1 mM MnCl2, enzyme from patient with enzyme deficiency | Homo sapiens | |
3.4.13.9 | 9.9 | - |
L-Pro-L-Met | pH 7.8, 37°C, presence of 0.1 mM MnCl2 plus 20 mM glycine | Homo sapiens | |
3.4.13.9 | 10.1 | - |
L-Pro-L-Val | pH 7.8, 37°C, presence of 0.1 mM MnCl2, enzyme from patient with enzyme deficiency | Homo sapiens | |
3.4.13.9 | 13.8 | - |
L-Pro-Gly | pH 7.8, 37°C, presence of 0.1 mM MnCl2 | Homo sapiens | |
3.4.13.9 | 14.7 | - |
L-Pro-L-Val | pH 7.8, 37°C, presence of 20 mM glycine | Homo sapiens | |
3.4.13.9 | 15.1 | - |
L-Pro-Gly | pH 7.8, 37°C, presence of 20 mM glycine | Homo sapiens | |
3.4.13.9 | 16.7 | - |
L-Pro-Gly | pH 7.8, 37°C, presence of 20 mM glycine, enzyme from patient with enzyme deficiency | Homo sapiens | |
3.4.13.9 | 17.3 | - |
L-Pro-L-Val | pH 7.8, 37°C, presence of 0.1 mM MnCl2 plus 20 mM glycine | Homo sapiens | |
3.4.13.9 | 17.6 | - |
L-Pro-L-Met | pH 7.8, 37°C | Homo sapiens | |
3.4.13.9 | 18.2 | - |
L-Pro-Gly | pH 7.8, 37°C, enzyme from patient with enzyme deficiency | Homo sapiens | |
3.4.13.9 | 19.1 | - |
L-Pro-L-Met | pH 7.8, 37°C, presence of 0.1 mM MnCl2 | Homo sapiens | |
3.4.13.9 | 26 | - |
L-Pro-L-Val | pH 7.8, 37°C | Homo sapiens | |
3.4.13.9 | 26.9 | - |
L-Pro-Gly | pH 7.8, 37°C | Homo sapiens | |
3.4.13.9 | 28.6 | - |
L-Pro-L-Val | pH 7.8, 37°C, presence of 0.1 mM MnCl2 | Homo sapiens | |
3.4.13.18 | 3.2 | - |
Pro-Val | pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 20 mM Gly | Homo sapiens | |
3.4.13.18 | 4.2 | - |
Pro-Met | pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 20 mM Gly | Homo sapiens | |
3.4.13.18 | 5 | - |
Pro-Met | pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 and 20 mM Gly | Homo sapiens | |
3.4.13.18 | 5.5 | - |
Pro-Val | pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 and 20 mM Gly | Homo sapiens | |
3.4.13.18 | 6.8 | - |
Pro-Gly | pH 7.8, 37°, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2 and 20 mM Gly | Homo sapiens | |
3.4.13.18 | 7.9 | - |
Pro-Val | pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, without activating compound | Homo sapiens | |
3.4.13.18 | 8.1 | - |
Pro-Met | pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, without activating compound | Homo sapiens | |
3.4.13.18 | 8.35 | - |
Pro-Met | pH 7.8, 37°C, erythrocyte lysate from healthy patients, activated by 20 mM Gly | Homo sapiens | |
3.4.13.18 | 8.6 | - |
Pro-Gly | pH 7.8, 37°, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 and 20 mM Gly | Homo sapiens | |
3.4.13.18 | 9.7 | - |
Pro-Gly | pH 7.8, 37°, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 | Homo sapiens | |
3.4.13.18 | 9.7 | - |
Pro-Met | pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 | Homo sapiens | |
3.4.13.18 | 9.9 | - |
Pro-Met | pH 7.8, 37°C, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2 and 20 mM Gly | Homo sapiens | |
3.4.13.18 | 10.1 | - |
Pro-Val | pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 | Homo sapiens | |
3.4.13.18 | 13.8 | - |
Pro-Gly | pH 7.8, 37°, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2 | Homo sapiens | |
3.4.13.18 | 14.7 | - |
Pro-Val | pH 7.8, 37°C, erythrocxyte lysate from healthy patients, activated by 20 mM Gly | Homo sapiens | |
3.4.13.18 | 15.1 | - |
Pro-Gly | pH 7.8, 37°, erythrocyte lysate from healthy patients, activated by 20 mM Gly | Homo sapiens | |
3.4.13.18 | 16.7 | - |
Pro-Gly | pH 7.8, 37°, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 20 mM Gly | Homo sapiens | |
3.4.13.18 | 17.3 | - |
Pro-Val | pH 7.8, 37°C, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2 and 20 mM Gly | Homo sapiens | |
3.4.13.18 | 17.6 | - |
Pro-Met | pH 7.8, 37°C, erythrocyte lysate from healthy patients, without activating compound | Homo sapiens | |
3.4.13.18 | 18.2 | - |
Pro-Gly | pH 7.8, 37°, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, without activating compound | Homo sapiens | |
3.4.13.18 | 19.1 | - |
Pro-Met | pH 7.8, 37°C, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2 | Homo sapiens | |
3.4.13.18 | 26 | - |
Pro-Val | pH 7.8, 37°C, erythrocyte lysate from healthy patients, without activating compound | Homo sapiens | |
3.4.13.18 | 26.9 | - |
Pro-Gly | pH 7.8, 37°, erythrocyte lysate from healthy patients, without activating compound | Homo sapiens | |
3.4.13.18 | 28.6 | - |
Pro-Val | pH 7.8, 37°C, erythrocxyte lysate from healthy patients, activated by 0.1 mM MnCl2 | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.13.18 | Mn2+ | 0.1 mM, increases prolinase activity in normal erythrocytes against Pro-Gly, Pro-Glu, Pro-Leu, Pro-Ser and Pro-Phe | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.13.9 | Homo sapiens | - |
comparison of normal human and a patient with prolidase deficiency | - |
3.4.13.18 | Homo sapiens | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.13.18 | erythrocyte | lysate | Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.13.9 | L-Pro-Gly + H2O | - |
Homo sapiens | L-Pro + Gly | - |
? | |
3.4.13.9 | L-Pro-L-Ala + H2O | - |
Homo sapiens | L-Pro + L-Ala | - |
? | |
3.4.13.9 | L-Pro-L-Asp + H2O | - |
Homo sapiens | L-Pro + L-Asp | - |
? | |
3.4.13.9 | L-Pro-L-Glu + H2O | - |
Homo sapiens | L-Pro + L-Glu | - |
? | |
3.4.13.9 | L-Pro-L-Leu + H2O | - |
Homo sapiens | L-Pro + L-Leu | - |
? | |
3.4.13.9 | L-Pro-L-Met + H2O | - |
Homo sapiens | L-Pro + L-Met | - |
? | |
3.4.13.9 | L-Pro-L-Phe + H2O | - |
Homo sapiens | L-Pro + L-Phe | - |
? | |
3.4.13.9 | L-Pro-L-Ser + H2O | - |
Homo sapiens | L-Pro + L-Ser | - |
? | |
3.4.13.9 | L-Pro-L-Val + H2O | - |
Homo sapiens | L-Pro + L-Val | - |
? | |
3.4.13.18 | Pro-Ala + H2O | - |
Homo sapiens | Pro + Ala | - |
? | |
3.4.13.18 | Pro-Asp + H2O | - |
Homo sapiens | Pro + Asp | - |
? | |
3.4.13.18 | Pro-Glu + H2O | - |
Homo sapiens | Pro + Glu | - |
? | |
3.4.13.18 | Pro-Gly + H2O | - |
Homo sapiens | Pro + Gly | - |
? | |
3.4.13.18 | Pro-Leu + H2O | - |
Homo sapiens | Pro + Leu | - |
? | |
3.4.13.18 | Pro-Met + H2O | - |
Homo sapiens | Pro + Met | - |
? | |
3.4.13.18 | Pro-Phe + H2O | - |
Homo sapiens | Pro + Phe | - |
? | |
3.4.13.18 | Pro-Ser + H2O | - |
Homo sapiens | Pro + Ser | - |
? | |
3.4.13.18 | Pro-Val + H2O | - |
Homo sapiens | Pro + Val | - |
? |