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Literature summary extracted from

  • Yang, Z.; Colis, L.C.; Basu, A.K.; Zou, Y.
    Recognition and incision of gamma-radiation-induced cross-linked guanine-thymine tandem lesion G[8,5-Me]T by UvrABC nuclease (2005), Chem. Res. Toxicol., 18, 1339-1346.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.25.1 overexpression of UvrA, UvrB, and UvrC Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.25.1 additional information
-
 additional information DNA-enzyme binding kinetics Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.25.1 KCl
-
 Escherichia coli
3.1.25.1 Mg2+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.25.1 additional information Escherichia coli the enzyme is involved in nucleotide excision repair of damaged DNA removing various types of bulky and distorting DNA adducts induced by UV radiation, process initiation is performed by UvrA, nicking by UvrB, which also recruits UvrC that catalyzes both the 3' and 5' incisions ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.25.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.25.1 recombinant UvrA, UvrB, and UvrC Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.25.1 endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate reaction mechanisms of UvrA, UvrB, and UvrC Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.25.1 DNA with a C8 guanine adduct of N-acetyl-2-aminofluorene + H2O
-
 Escherichia coli ?
-
 ?
3.1.25.1 DNA with gamma-radiation-induced cross-linked guanine-thymine tandem lesion G[8,5-Me]T + H2O the DNA substrate contains a covalent bond between C8 of the guanine and the 5-methyl carbon of the 3'-thymine, formed by X-radiation Escherichia coli ?
-
 ?
3.1.25.1 DNA with gamma-radiation-induced cross-linked thymine-thymine lesion T[6,4]T + H2O 6-4 pyrimidine-pyrimidone photoproduct Escherichia coli ?
-
 ?
3.1.25.1 additional information the enzyme is involved in nucleotide excision repair of damaged DNA removing various types of bulky and distorting DNA adducts induced by UV radiation, process initiation is performed by UvrA, nicking by UvrB, which also recruits UvrC that catalyzes both the 3' and 5' incisions Escherichia coli ?
-
 ?
3.1.25.1 additional information the enzyme is involved in nucleotide excision repair of damaged DNA removing various types of bulky and distorting DNA adducts induced by UV radiation, process initiation is performed by UvrA, nicking by UvrB, which also recruits UvrC that catalyzes both the 3' and 5' incisions, substrate recognition and reaction mechanism, overview Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.25.1 UvrA
-
 Escherichia coli
3.1.25.1 UvrABC nuclease
-
 Escherichia coli
3.1.25.1 UvrB
-
 Escherichia coli
3.1.25.1 UvrC
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.25.1 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.25.1 7.5
-
 assay at Escherichia coli