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Literature summary extracted from

  • Piret, J.; Schanck, A.; Delfosse, S.; Van Bambeke, F.; Kishore, B.K.; Tulkens, P.M.; Mingeot-Leclercq, M.P.
    Modulation of the in vitro activity of lysosomal phospholipase A1 by membrane lipids (2005), Chem. Phys. Lipids, 133, 1-15.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.32 1.5
-
 phosphatidylcholine native vesicles, pH 5.4, 37°C Rattus norvegicus
3.1.1.32 4
-
 phosphatidylcholine delipidated vesicles, pH 5.4, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.32 lysosome whole-soluble lysosomal fraction Rattus norvegicus 5764
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.32 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.32 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.32 additional information
-
 enzyme activity is markedly enhanced when the amount of negatively-charged lipids included in the vesicles is increased from 10 to around 30% of total phospholipids and the intensity of this effect depends on the nature of the acidic lipids used, ganglioside GM1 Rattus norvegicus
3.1.1.32 80.2
-
 native vesicles, pH 5.4, 37°C Rattus norvegicus
3.1.1.32 155
-
 delipidated vesicles, pH 5.4, 37°C Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.32 phosphatidylcholine + H2O
-
 Rattus norvegicus 2-acylglycerophosphocholine + a carboxylate
-
 ?
3.1.1.32 phosphatidylethanolamine + H2O
-
 Rattus norvegicus 2-acylglycerophosphoethanolamine + a carboxylate
-
 ?