BRENDA - Enzyme Database

Modulation of the in vitro activity of lysosomal phospholipase A1 by membrane lipids

Piret, J.; Schanck, A.; Delfosse, S.; Van Bambeke, F.; Kishore, B.K.; Tulkens, P.M.; Mingeot-Leclercq, M.P.; Chem. Phys. Lipids 133, 1-15 (2005)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.32
1.5
-
phosphatidylcholine
native vesicles, pH 5.4, 37░C
Rattus norvegicus
3.1.1.32
4
-
phosphatidylcholine
delipidated vesicles, pH 5.4, 37░C
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.32
lysosome
whole-soluble lysosomal fraction
Rattus norvegicus
5764
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.32
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.32
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.1.32
additional information
-
enzyme activity is markedly enhanced when the amount of negatively-charged lipids included in the vesicles is increased from 10 to around 30% of total phospholipids and the intensity of this effect depends on the nature of the acidic lipids used, ganglioside GM1
Rattus norvegicus
3.1.1.32
80.2
-
native vesicles, pH 5.4, 37░C
Rattus norvegicus
3.1.1.32
155
-
delipidated vesicles, pH 5.4, 37░C
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.32
phosphatidylcholine + H2O
-
664591
Rattus norvegicus
2-acylglycerophosphocholine + a carboxylate
-
-
-
?
3.1.1.32
phosphatidylethanolamine + H2O
-
664591
Rattus norvegicus
2-acylglycerophosphoethanolamine + a carboxylate
-
-
-
?
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.32
1.5
-
phosphatidylcholine
native vesicles, pH 5.4, 37░C
Rattus norvegicus
3.1.1.32
4
-
phosphatidylcholine
delipidated vesicles, pH 5.4, 37░C
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.32
lysosome
whole-soluble lysosomal fraction
Rattus norvegicus
5764
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.32
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.1.32
additional information
-
enzyme activity is markedly enhanced when the amount of negatively-charged lipids included in the vesicles is increased from 10 to around 30% of total phospholipids and the intensity of this effect depends on the nature of the acidic lipids used, ganglioside GM1
Rattus norvegicus
3.1.1.32
80.2
-
native vesicles, pH 5.4, 37░C
Rattus norvegicus
3.1.1.32
155
-
delipidated vesicles, pH 5.4, 37░C
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.32
phosphatidylcholine + H2O
-
664591
Rattus norvegicus
2-acylglycerophosphocholine + a carboxylate
-
-
-
?
3.1.1.32
phosphatidylethanolamine + H2O
-
664591
Rattus norvegicus
2-acylglycerophosphoethanolamine + a carboxylate
-
-
-
?