EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.190 | Cu2+ | 10 mM, 94% inhibition | Sus scrofa | |
3.2.1.190 | Fe3+ | 10 mM, 88% inhibition | Sus scrofa | |
3.2.1.190 | Mg2+ | inhibits at 10-100 mM, slightly activates at 200 mM | Sus scrofa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.190 | Ca2+ | 10-200 mM, 1.13-1.18fold activation | Sus scrofa | |
3.2.1.190 | Mg2+ | inhibits at 10-100 mM, slightly activates (109% of the control activity) at 200 mM | Sus scrofa | |
3.2.1.190 | Zn2+ | 10-200 mM, 1.08-1.1fold activation | Sus scrofa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.190 | 47000 | - |
x * 47000, SDS-PAGE | Sus scrofa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.190 | 3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 2 H2O | Sus scrofa | the enzyme is involved in hydrolysis of the steroid saponin dioscin | 2 L-rhamnopyranose + diosgenin 3-O-beta-D-glucopyranoside | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.190 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.190 | - |
Sus scrofa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.190 | liver | - |
Sus scrofa | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.190 | 88 | - |
pH 7.2, 37°C | Sus scrofa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.190 | 3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 2 H2O | the enzyme is involved in hydrolysis of the steroid saponin dioscin | Sus scrofa | 2 L-rhamnopyranose + diosgenin 3-O-beta-D-glucopyranoside | - |
? | |
3.2.1.190 | 3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 2 H2O | - |
Sus scrofa | 2 L-rhamnopyranose + diosgenin-3-O-beta-D-glucopyranoside | - |
? | |
3.2.1.190 | additional information | no hydrolysis of ginsenoside Re or rutin | Sus scrofa | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.190 | ? | x * 47000, SDS-PAGE | Sus scrofa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.190 | dioscin alpha-L-rhamnosidase | - |
Sus scrofa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.190 | 42 | - |
- |
Sus scrofa |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.190 | 32 | 57 | 32°C: about 85% of maximal activity, 57°C: about 35% of maximal activity | Sus scrofa |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.190 | 32 | 52 | stable | Sus scrofa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.190 | 7 | - |
- |
Sus scrofa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.190 | 4 | 9 | pH 4.0: about 50% of maximal activity, pH 9.0: about 60% of maximal activity | Sus scrofa |