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Literature summary extracted from

  • Saario, S.M.; Salo, O.M.; Nevalainen, T.; Poso, A.; Laitinen, J.T.; Jarvinen, T.; Niemi, R.
    Characterization of the sulfhydryl-sensitive site in the enzyme responsible for hydrolysis of 2-arachidonoyl-glycerol in rat cerebellar membranes (2005), Chem. Biol., 12, 649-656.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.23 4-chlormercurybenzoate IC50 value is 0.072 mM Rattus norvegicus
3.1.1.23 HgCl2 IC50 value is 0.042 mM Rattus norvegicus
3.1.1.23 Maleimide IC50 value is 0.070 mM Rattus norvegicus
3.1.1.23 additional information inhibition by bulky maleimides derivatives is due to steric hindrance of substrate binding after alkylation of cysteines 242 and/or 208, no inhibition by succimide and N-arachidonylsuccimide Rattus norvegicus
3.1.1.23 N-(1-pyrenyl)-maleimide IC50 value is 0.068 mM Rattus norvegicus
3.1.1.23 N-arachidonoylmaleimide IC50 value is 0.00014 mM Rattus norvegicus
3.1.1.23 N-cyclohexylmaleimide IC50 value is 0.051 mM Rattus norvegicus
3.1.1.23 N-hydroxymaleimide IC50 value is 0.413 mM Rattus norvegicus
3.1.1.23 N-Phenylmaleimide IC50 value is 0.044 mM Rattus norvegicus
3.1.1.23 N-propylmaleimide IC50 value is 0.053 mM Rattus norvegicus
3.1.1.23 NEM IC50 value is 0.053 mM Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.23 membrane
-
 Rattus norvegicus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.23 2-arachidonoylglycerol + H2O Rattus norvegicus
-
 arachidonic acid + glycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.23 Rattus norvegicus Q8R431
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.23 cerebellum
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.23 2-arachidonoylglycerol + H2O
-
 Rattus norvegicus arachidonic acid + glycerol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.23 More structure molecular modeling from amino acid sequence Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.23 MGL-like activity
-
 Rattus norvegicus
3.1.1.23 monoglyceride lipase-like activity
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.23 25
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.23 7.4
-
 assay at Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.1.23 0.00014
-
 IC50 value is 0.00014 mM Rattus norvegicus N-arachidonoylmaleimide
3.1.1.23 0.042
-
 IC50 value is 0.042 mM Rattus norvegicus HgCl2
3.1.1.23 0.044
-
 IC50 value is 0.044 mM Rattus norvegicus N-Phenylmaleimide
3.1.1.23 0.051
-
 IC50 value is 0.051 mM Rattus norvegicus N-cyclohexylmaleimide
3.1.1.23 0.053
-
 IC50 value is 0.053 mM Rattus norvegicus NEM
3.1.1.23 0.053
-
 IC50 value is 0.053 mM Rattus norvegicus N-propylmaleimide
3.1.1.23 0.068
-
 IC50 value is 0.068 mM Rattus norvegicus N-(1-pyrenyl)-maleimide
3.1.1.23 0.07
-
 IC50 value is 0.070 mM Rattus norvegicus Maleimide
3.1.1.23 0.072
-
 IC50 value is 0.072 mM Rattus norvegicus 4-chlormercurybenzoate
3.1.1.23 0.413
-
 IC50 value is 0.413 mM Rattus norvegicus N-hydroxymaleimide