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Literature summary extracted from
- Production and characterization of erythropoietic protoporphyric heterodimeric ferrochelatases (2005), Blood, 106, 1098-1104.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.98.1.1 | expression in Escherichia coli JM109 | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.98.1.1 | C406S | complements the ferrochelatase-deficient delta-hemH Escherichia coli, no activity | Homo sapiens |
| 4.98.1.1 | F417S | complements the ferrochelatase-deficient delta-hemH Escherichia coli, no activity | Homo sapiens |
| 4.98.1.1 | H386P | does not complement the ferrochelatase-deficient delta-hemH Escherichia coli, the heterodimeric form (co-expressed with wild-type) cannot be isolated as pure protein, no activity | Homo sapiens |
| 4.98.1.1 | M288K | does not complement the ferrochelatase-deficient delta-hemH Escherichia coli, the heterodimeric form (co-expressed with wild-type) cannot be isolated as pure protein, no activity | Homo sapiens |
| 4.98.1.1 | R115L | exhibits wild-type kinetics, but is more stable than the wild-type | Homo sapiens |
| 4.98.1.1 | S264L | does not complement the ferrochelatase-deficient delta-hemH Escherichia coli | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.98.1.1 | 0.0061 | - |
mesoporphyrin IX | mutant M267I, heterodimer, room temperature | Homo sapiens |  |
| 4.98.1.1 | 0.0083 | - |
Fe2+ | mutant M267I, heterodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0092 | - |
mesoporphyrin IX | mutant Q139L, heterodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0094 | - |
mesoporphyrin IX | wild-type, heterodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0097 | - |
mesoporphyrin IX | mutant F260L, heterodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0114 | - |
Fe2+ | wild-type, heterodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0117 | - |
Fe2+ | mutant M267I, homodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0119 | - |
Fe2+ | wild-type, homodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0121 | - |
mesoporphyrin IX | wild-type, homodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0123 | - |
mesoporphyrin IX | mutant M267I, homodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0129 | - |
mesoporphyrin IX | mutant C236Y, heterodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0129 | - |
mesoporphyrin IX | mutant Y191H, homodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0138 | - |
mesoporphyrin IX | mutant S264L, heterodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0141 | - |
Fe2+ | mutant C236Y, homodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0146 | - |
mesoporphyrin IX | mutant F260L, homodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0172 | - |
mesoporphyrin IX | mutant Y191H, heterodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0179 | - |
Fe2+ | mutant C236Y, heterodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0182 | - |
mesoporphyrin IX | mutant P334L, homodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0219 | - |
Fe2+ | mutant Q139L, heterodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0224 | - |
mesoporphyrin IX | mutant K379N, homodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0232 | - |
Fe2+ | mutant K379N, heterodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0235 | - |
Fe2+ | mutant S264L, heterodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0251 | - |
Fe2+ | mutant Y191H, homodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0253 | - |
mesoporphyrin IX | mutant K379N, heterodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0262 | - |
Fe2+ | mutant Q139L, homodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0267 | - |
mesoporphyrin IX | mutant Q139L, homodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0302 | - |
mesoporphyrin IX | mutant C236Y, homodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.038 | - |
Fe2+ | mutant Y191H, heterodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0441 | - |
Fe2+ | mutant F260L, heterodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0488 | - |
Fe2+ | mutant P334L, homodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.0763 | - |
Fe2+ | mutant F260L, homodimer, room temperature | Homo sapiens | |
| 4.98.1.1 | 0.1101 | - |
Fe2+ | mutant K379N, homodimer, room temperature | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.98.1.1 | 90000 | - |
mutant C406S, dimer, dynamic light scattering | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.98.1.1 | protoporphyrin + Fe2+ | Homo sapiens | - |
protoheme + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.98.1.1 | Homo sapiens | P22830 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.98.1.1 | the recombinant enzyme | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.98.1.1 | mesoporphyrin IX + ferrous ammonium sulfate | room temperature | Homo sapiens | mesoheme + ammonium sulfate | - |
? | |
| 4.98.1.1 | protoporphyrin + Fe2+ | - |
Homo sapiens | protoheme + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.98.1.1 | dimer | wild-type, gel filtration | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.98.1.1 | ferrochelatase | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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