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Literature summary extracted from

  • Parthasarathy, R.; Bajaj, J.; Boder, E.T.
    An immobilized biotin ligase: surface display of Escherichia coli BirA on Saccharomyces cerevisiae (2005), Biotechnol. Prog., 21, 1627-1631.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.4.15 synthesis the enzyme is utilized to synthesize site-specific biotinylated proteins via a biotin acceptor peptide tag Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.15 gene birA, functional expression of N-terminally and C-terminally multiply tagged enzyme in Saccharomyces cerevisiae, low level expression of the surface-displayed enzyme, expression of avitagged-BirA in Saccharomyces cerevisiae, overview Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.15 glycerol inhibits the recombinant enzyme at 10% Escherichia coli
6.3.4.15 NaCl inhibits the purified enzyme at 0.1 M, but not the immobilized enzyme in crude extract of recombinant yeast cells Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.15 0.0033
-
 avitag-tagged maltose-binding protein recombinant enzyme Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.15 35000
-
 x * 35000, recombinant enzyme, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.15 avitag-tagged maltose-binding protein + biotin + ATP Escherichia coli recombinant Saccharomyces cerevisiae cells maltose-binding protein-avitag-biotin + AMP + diphosphate
-
 ?
6.3.4.15 avitagged BirA + biotin + ATP Escherichia coli recombinant Saccharomyces cerevisiae cells BirA-avitag-biotin + AMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.15 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.15 recombinant enzyme from Saccharomyces cerevisiae, immobilization of the recombinant enzyme Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.15 additional information
-
 the immobilized enzyme is slightly less active than the soluble enzyme Escherichia coli

Storage Stability

EC Number Storage Stability Organism
6.3.4.15 4°C, immobilized recombinant enzyme, PBS, completely stable for 4 weeks, loss of activity after 5 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.15 avitag-tagged maltose-binding protein + biotin + ATP recombinant Saccharomyces cerevisiae cells Escherichia coli maltose-binding protein-avitag-biotin + AMP + diphosphate
-
 ?
6.3.4.15 avitag-tagged maltose-binding protein + biotin + ATP recombinant avitag-tagged maltose-binding protein Escherichia coli maltose-binding protein-avitag-biotin + AMP + diphosphate
-
 ?
6.3.4.15 avitagged BirA + biotin + ATP recombinant Saccharomyces cerevisiae cells Escherichia coli BirA-avitag-biotin + AMP + diphosphate
-
 ?
6.3.4.15 avitagged BirA + biotin + biotin + ATP the recombinant avitagged enzyme biotinylates itself at the avitag sequence Escherichia coli BirA-avitag-biotin + AMP + diphosphate
-
 ?
6.3.4.15 additional information the enzyme site-specifically biotinylates proteins via a biotin acceptor peptide tag Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.15 ? x * 35000, recombinant enzyme, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
6.3.4.15 biotin ligase
-
 Escherichia coli
6.3.4.15 BirA
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.15 ATP
-
 Escherichia coli