EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.11.28 | expression in Escherichia coli | Streptomyces sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.28 | Co2+ | more than 50% inhibition at 1 mM | Streptomyces sp. | |
1.14.11.28 | Cu2+ | more than 50% inhibition at 1 mM | Streptomyces sp. | |
1.14.11.28 | EDTA | more than 50% inhibition at 1 mM | Streptomyces sp. | |
1.14.11.28 | Ni2+ | more than 50% inhibition at 1 mM | Streptomyces sp. | |
1.14.11.28 | Zn2+ | more than 50% inhibition at 1 mM | Streptomyces sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.11.28 | 0.047 | - |
2-oxoglutarate | - |
Streptomyces sp. | |
1.14.11.28 | 0.083 | - |
2-oxoglutarate | - |
Streptomyces sp. | |
1.14.11.28 | 0.2 | - |
L-proline | - |
Streptomyces sp. | |
1.14.11.28 | 0.43 | - |
L-proline | - |
Streptomyces sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.28 | Fe2+ | strictly required | Streptomyces sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.11.28 | 33150 | - |
calculated | Streptomyces sp. |
1.14.11.28 | 33570 | - |
calculated | Streptomyces sp. |
1.14.11.28 | 35000 | - |
apparent molecular weight on SDS-PAGE | Streptomyces sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.11.28 | Streptomyces sp. | - |
strainTH1 | - |
1.14.11.28 | Streptomyces sp. TH1 | - |
strainTH1 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.11.28 | 2 chromatographic steps to homogeneity | Streptomyces sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.11.28 | cell culture | - |
Streptomyces sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.11.28 | 7.2 | - |
- |
Streptomyces sp. |
1.14.11.28 | 12.7 | - |
- |
Streptomyces sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.28 | 3,4-dehydro-L-proline + 2-oxoglutarate + O2 | - |
Streptomyces sp. | cis-3,4-epoxy-L-proline + succinate + CO2 | - |
? | |
1.14.11.28 | 3,4-dehydro-L-proline + 2-oxoglutarate + O2 | - |
Streptomyces sp. TH1 | cis-3,4-epoxy-L-proline + succinate + CO2 | - |
? | |
1.14.11.28 | L-2-azetidinecarboxylic acid + 2-oxoglutarate + O2 | - |
Streptomyces sp. | cis-3-hydroxyazetidine-L-2-carboxylic acid + succinate + CO2 | - |
? | |
1.14.11.28 | L-2-azetidinecarboxylic acid + 2-oxoglutarate + O2 | - |
Streptomyces sp. TH1 | cis-3-hydroxyazetidine-L-2-carboxylic acid + succinate + CO2 | - |
? | |
1.14.11.28 | L-pipecolic acid + 2-oxoglutarate + O2 | - |
Streptomyces sp. | cis-3-hydroxy-L-pipecolic acid + succinate + CO2 | - |
? | |
1.14.11.28 | L-pipecolic acid + 2-oxoglutarate + O2 | - |
Streptomyces sp. TH1 | cis-3-hydroxy-L-pipecolic acid + succinate + CO2 | - |
? | |
1.14.11.28 | L-proline + 2-oxoglutarate + O2 | - |
Streptomyces sp. | cis-3-hydroxy-L-proline + succinate + CO2 | - |
? | |
1.14.11.28 | L-proline + 2-oxoglutarate + O2 | - |
Streptomyces sp. TH1 | cis-3-hydroxy-L-proline + succinate + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.11.28 | proline 3-hydroxylase type I | - |
Streptomyces sp. |
1.14.11.28 | proline 3-hydroxylase type II | - |
Streptomyces sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.11.28 | 35 | - |
- |
Streptomyces sp. |
1.14.11.28 | 40 | - |
- |
Streptomyces sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.11.28 | 30 | - |
below 30°C activity remained above 85% after 30 min at pH 6.0 | Streptomyces sp. |
1.14.11.28 | 40 | - |
below 40°C activity remained above 85% after 30 min at pH 6.0 | Streptomyces sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.11.28 | 6 | - |
- |
Streptomyces sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.14.11.28 | 5.5 | 9 | activity remained above 85% after 16 h at 4°C | Streptomyces sp. |
1.14.11.28 | 5.5 | 8.5 | activity remained above 85% after 16 h at 4°C | Streptomyces sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.28 | 2-oxoglutarate | strictly required | Streptomyces sp. |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.14.11.28 | Streptomyces sp. | - |
- |
4.3 |
1.14.11.28 | Streptomyces sp. | - |
- |
4.6 |