Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Migita, K.; Nishimura, T.
    Purification and characterization of a Cl--activated aminopeptidase from bovine skeletal muscle (2006), Biosci. Biotechnol. Biochem., 70, 1110-1117.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.11.2 dithiothreitol 307% activity in the presence of 10 mM Bos taurus
3.4.11.2 DTT activates 3fold at 10 mM Bos taurus
3.4.11.2 EDTA 146% activity in the presence of 10 mM Bos taurus
3.4.11.2 ethanol 116% activity in the presence of 1 mM Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.2 2-mercaptoethanol 21.6% inhibition at 10 mM; 78.4% residual activity in the presence of 10 mM Bos taurus
3.4.11.2 bestatin 3.2% residual activity in the presence of 1 mM; 97% inhibition at 1 mM Bos taurus
3.4.11.2 Co2+ 2% residual activity in the presence of 1 mM; 98% inhibition at 1 mM Bos taurus
3.4.11.2 Cu2+ 1.6% residual activity in the presence of 1 mM; 98.4% inhibition at 1 mM Bos taurus
3.4.11.2 Fe3+ 39.9% residual activity in the presence of 1 mM; 60.1% inhibition at 1 mM Bos taurus
3.4.11.2 iodoacetate 38.8% residual activity in the presence of 10 mM Bos taurus
3.4.11.2 iodoacetic acid 61.2% inhibition at 10 mM Bos taurus
3.4.11.2 K+ 17.7% inhibition at 1 mM; 82.3% residual activity in the presence of 1 mM Bos taurus
3.4.11.2 Mg2+ 45.1% inhibition at 1 mM; 54.9% residual activity in the presence of 1 mM Bos taurus
3.4.11.2 Mn2+ 5% residual activity in the presence of 1 mM; 94.9% inhibition at 1 mM Bos taurus
3.4.11.2 pepstatin A 22.3% inhibition at 1 mM; 77.7% residual activity in the presence of 1 mM Bos taurus
3.4.11.2 Phenylmethylsulfonylfluoride complete inhibition in the presence of 1 mM Bos taurus
3.4.11.2 PMSF complete inhibition at 1 mM Bos taurus
3.4.11.2 puromycin 25.8% residual activity in the presence of 1 mM; 74.2% inhibition at 1 mM Bos taurus
3.4.11.2 SCN- 4.2% residual activity in the presence of 1 mM Bos taurus
3.4.11.2 SO42- 14% residual activity in the presence of 1 mM Bos taurus
3.4.11.2 sulfate 86% inhibition at 1 M Bos taurus
3.4.11.2 Thiocyanide 95.8% inhibition at 1 M Bos taurus
3.4.11.2 Zn2+ 2.2% residual activity in the presence of 1 mM; 97.8% inhibition at 1 mM Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.2 Ca2+ 44% activation at 1 mM Bos taurus
3.4.11.2 Ca2+ 144% activity in the presence of 1 mM Bos taurus
3.4.11.2 Cl- 3.6fold activation at 1 M Bos taurus
3.4.11.2 Cl- 369% activity in the presence of 1 mM Bos taurus
3.4.11.2 F- 107% activity in the presence of 1 mM Bos taurus
3.4.11.2 additional information no effect by F- Bos taurus
3.4.11.2 nitrate 3.25fold activation at 1 M Bos taurus
3.4.11.2 NO3- 325% activity in the presence of 1 mM Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.2 58000
-
 SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.2 ammonium sulfate fractionation, DEAE-cellulose column chromatography, Sephacryl S-200 gel filtration, phenyl-Sepharose column chromatography, and His-Trap affinity column chromatography Bos taurus
3.4.11.2 native enzyme 659fold from skeletal muscle by ammonium sulfate fractionation, anion exchange chromatography, hydroxyapatite chromatography, hydrophobic interaction chromatography, and zinc affinity chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.2 skeletal muscle
-
 Bos taurus
-
3.4.11.2 skeletal muscle Musculus longissimus thoracis Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.2 659
-
 purified enzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 Ala-beta-naphthylamide + H2O 100% activity, preferred substrate Bos taurus Ala + beta-naphthylamine
-
 ?
3.4.11.2 Arg-beta-naphthylamide + H2O 16.9% activity compared to Ala-beta-naphthylamide Bos taurus Arg + beta-naphthylamine
-
 ?
3.4.11.2 L-Ala-2-naphthylamide + H2O preferred substrate Bos taurus L-Ala + 2-naphthylamine
-
 ?
3.4.11.2 L-Arg-2-naphthylamide + H2O 16.9% of the activity with L-Ala-2-naphthylamide Bos taurus L-Arg + 2-naphthylamine
-
 ?
3.4.11.2 L-Leu-2-naphthylamide + H2O 16.0% of the activity with L-Ala-2-naphthylamide Bos taurus L-Leu + 2-naphthylamine
-
 ?
3.4.11.2 L-Lys-2-naphthylamide + H2O 6.7% of the activity with L-Ala-2-naphthylamide Bos taurus L-Lys + 2-naphthylamine
-
 ?
3.4.11.2 L-Met-2-naphthylamide + H2O 8.9% of the activity with L-Ala-2-naphthylamide Bos taurus L-Met + 2-naphthylamine
-
 ?
3.4.11.2 L-Pro-2-naphthylamide + H2O 16.1% of the activity with L-Ala-2-naphthylamide Bos taurus L-Pro + 2-naphthylamine
-
 ?
3.4.11.2 Leu-beta-naphthylamide + H2O 16% activity compared to Ala-beta-naphthylamide Bos taurus Leu + beta-naphthylamine
-
 ?
3.4.11.2 Lys-beta-naphthylamide + H2O 6.7% activity compared to Ala-beta-naphthylamide Bos taurus Lys + beta-naphthylamine
-
 ?
3.4.11.2 Met-beta-naphthylamide + H2O 8.9% activity compared to Ala-beta-naphthylamide Bos taurus Met + beta-naphthylamine
-
 ?
3.4.11.2 additional information L-Ser-2-naphthylamide is a poor substrate, L-Glu-2-naphthylamide is no substrate Bos taurus ?
-
 ?
3.4.11.2 additional information no activity with Glu-beta-naphthylamide Bos taurus ?
-
 ?
3.4.11.2 Pro-beta-naphthylamide + H2O 16.1% activity compared to Ala-beta-naphthylamide Bos taurus Pro + beta-naphthylamine
-
 ?
3.4.11.2 Ser-beta-naphthylamide + H2O 1.4% activity compared to Ala-beta-naphthylamide Bos taurus Ser + beta-naphthylamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.2 ? x * 58000, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.2 aminopeptidase D
-
 Bos taurus
3.4.11.2 APAse D
-
 Bos taurus
3.4.11.2 Cl--activated aminopeptidase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.2 37
-
 assay at Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.2 55
-
 10 min, purified enzyme, stable Bos taurus
3.4.11.2 55 65 stable up to 55°C, but stability is almost lost at 65°C Bos taurus
3.4.11.2 65
-
 10 min, purified enzyme, almost loss of activity Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.2 7.5
-
-
 Bos taurus
3.4.11.2 7.5
-
 around pH 7.5 Bos taurus