EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.21 | biotechnology | immobilization of the enzyme on gelatin, overview, the very stable gelatin-immobilized enzyme can be used in continuous synthesis of beta-glucosides by transglucosylation | Pyrococcus furiosus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.21 | 4-nitrophenol | the gelatin-immobilized enzyme is less sensitive than the native enzyme | Pyrococcus furiosus | |
3.2.1.21 | D-glucose | the gelatin-immobilized enzyme is less sensitive than the native enzyme | Pyrococcus furiosus | |
3.2.1.21 | phenol | the gelatin-immobilized enzyme is not sensitive in contrast to the native enzyme | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.21 | Pyrococcus furiosus | Q51723 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | additional information | - |
- |
Pyrococcus furiosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.21 | additional information | the gelatin-immobilized enzyme also catalyzes the transfer of beta-D-glucose from cellobiose to 5-phenylpentanol forming 5-phenylpentyl beta-D-glucopyranoside | Pyrococcus furiosus | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 70 | 90 | assay at | Pyrococcus furiosus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | additional information | - |
hyperthermostable native enzyme, thermostability is even increased by immobilization on gelatin | Pyrococcus furiosus |
3.2.1.21 | 90 | - |
enzyme immobilized on gelatin, completely stable for one month | Pyrococcus furiosus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 5 | - |
assay at | Pyrococcus furiosus |