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Literature summary extracted from
- Purification, characterization, and subsite affinities of Thermoactinomyces vulgaris R-47 maltooligosaccharide-metabolizing enzyme homologous to glucoamylases (2004), Biosci. Biotechnol. Biochem., 68, 413-420.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.3 | expression in Escherichia coli strain MV1184 | Thermoactinomyces vulgaris |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.3 | additional information | - |
additional information | kinetics, recombinant enzyme | Thermoactinomyces vulgaris | |
| 3.2.1.3 | 0.101 | - |
maltose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris |  |
| 3.2.1.3 | 0.38 | - |
amylotriose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
| 3.2.1.3 | 1.03 | - |
maltotetraose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
| 3.2.1.3 | 3.94 | - |
maltopentaose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
| 3.2.1.3 | 7.43 | - |
maltohexaose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
| 3.2.1.3 | 7.59 | - |
maltoheptaose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.3 | Thermoactinomyces vulgaris | - |
- |
- |
| 3.2.1.3 | Thermoactinomyces vulgaris R-47 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.3 | recombinant enzyme from Escherichia coli strain MV1184 by heat treatment at 50°C for 30 min, two steps of ion exchange chromatography, and gel filtration | Thermoactinomyces vulgaris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.3 | maltoheptaose + H2O | - |
Thermoactinomyces vulgaris | maltohexaose + D-glucose | - |
? | |
| 3.2.1.3 | maltoheptaose + H2O | - |
Thermoactinomyces vulgaris R-47 | maltohexaose + D-glucose | - |
? | |
| 3.2.1.3 | maltononaose + H2O | - |
Thermoactinomyces vulgaris | maltooctaose + beta-D-glucose | - |
? | |
| 3.2.1.3 | maltooctaose + H2O | - |
Thermoactinomyces vulgaris | maltoheptaose + beta-D-glucose | - |
? | |
| 3.2.1.3 | maltopentadecaose + H2O | low activity | Thermoactinomyces vulgaris | maltotetradecaose + beta-D-glucose | - |
? | |
| 3.2.1.3 | maltopentaose + H2O | preferred substrate | Thermoactinomyces vulgaris | maltotetraose + beta-D-glucose | - |
? | |
| 3.2.1.3 | maltopentaose + H2O | preferred substrate | Thermoactinomyces vulgaris R-47 | maltotetraose + beta-D-glucose | - |
? | |
| 3.2.1.3 | maltose + H2O | - |
Thermoactinomyces vulgaris | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.3 | maltose + H2O | - |
Thermoactinomyces vulgaris R-47 | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.3 | maltotetraose + H2O | preferred substrate | Thermoactinomyces vulgaris | maltotriose + beta-D-glucose | - |
? | |
| 3.2.1.3 | maltotetraose + H2O | preferred substrate | Thermoactinomyces vulgaris R-47 | maltotriose + beta-D-glucose | - |
? | |
| 3.2.1.3 | maltotriose + H2O | - |
Thermoactinomyces vulgaris | maltose + beta-D-glucose | - |
? | |
| 3.2.1.3 | additional information | no activity with pullulan, dextran, and isomaltose | Thermoactinomyces vulgaris | ? | - |
? | |
| 3.2.1.3 | additional information | no activity with pullulan, dextran, and isomaltose | Thermoactinomyces vulgaris R-47 | ? | - |
? | |
| 3.2.1.3 | starch + H2O | soluble starch, very low activity | Thermoactinomyces vulgaris | starch + beta-D-glucose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.3 | GA | - |
Thermoactinomyces vulgaris |
| 3.2.1.3 | glucoamylase | - |
Thermoactinomyces vulgaris |
| 3.2.1.3 | maltooligosaccharide-metabolizing enzyme | - |
Thermoactinomyces vulgaris |
| 3.2.1.3 | tGA | - |
Thermoactinomyces vulgaris |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 60 | - |
- |
Thermoactinomyces vulgaris |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 60 | - |
stable up to | Thermoactinomyces vulgaris |
| 3.2.1.3 | 68 | - |
4.5% remaining activity | Thermoactinomyces vulgaris |
| 3.2.1.3 | 70 | - |
inactivation | Thermoactinomyces vulgaris |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.3 | 1.6 | - |
maltotetraose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
| 3.2.1.3 | 16.9 | - |
maltose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
| 3.2.1.3 | 55 | - |
maltohexaose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
| 3.2.1.3 | 56.3 | - |
maltopentaose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
| 3.2.1.3 | 61.6 | - |
maltoheptaose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
| 3.2.1.3 | 70.7 | - |
maltotetraose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
| 3.2.1.3 | 76.4 | - |
maltotriose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 6.8 | - |
- |
Thermoactinomyces vulgaris |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 5.5 | 9.5 | stable, recombinant enzyme | Thermoactinomyces vulgaris |
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