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Literature summary extracted from

  • Chon, H.; Nakano, R.; Ohtani, N.; Haruki, M.; Takano, K.; Morikawa, M.; Kanaya, S.
    Gene cloning and biochemical characterizations of thermostable ribonuclease HIII from Bacillus stearothermophilus (2004), Biosci. Biotechnol. Biochem., 68, 2138-2147.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.26.4 gene rnhC, DNA and amino acid sequence determination and analysis, overexpression of the soluble enzyme in Escherichia coli strain MIC2067(DE3) Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.26.4 Co2+ over 95% inhibition at 5 mM and below Geobacillus stearothermophilus
3.1.26.4 Mn2+ 40% inhibition at 100 mM Geobacillus stearothermophilus
3.1.26.4 Ni2+ nearly complete inhibition at 5 mM and below Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.4 KCl highly activating, best at 100-200 mM salt, KCl is preferred Geobacillus stearothermophilus
3.1.26.4 Mg2+ required, highest activity at 50 mM Geobacillus stearothermophilus
3.1.26.4 additional information Mg2+ cannot be substituted by Co2+ and Ni2+, and only partially by Mn2+ Geobacillus stearothermophilus
3.1.26.4 NaCl activating, best at 100-200 mM salt Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.4 33734
-
 1 * 33734, amino acid sequence calculation, 1 * 36000, recombinant enzyme, SDS-PAGE Geobacillus stearothermophilus
3.1.26.4 36000
-
 recombinant enzyme, gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.4 Geobacillus stearothermophilus Q6L6Q4 gene rnhC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.4 recombinant enzyme to homogeneity from Escherichia coli strain MIC2067(DE3) by ammonium sulfate precipitation, heparin affinity chromatography, and gel filtration Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.4 12 basepair DNA-DNA duplex + H2O oligomeric substrate, cleavage at multiple sites, product identification Geobacillus stearothermophilus ?
-
 ?
3.1.26.4 29 basepair DNA-RNA-DNA/DNA + H2O oligomeric substrate, cleavage mainly in the middle of the tetraribonucleotide, product identification Geobacillus stearothermophilus ?
-
 ?
3.1.26.4 M13 DNA-RNA hybrid + H2O
-
 Geobacillus stearothermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.26.4 monomer 1 * 33734, amino acid sequence calculation, 1 * 36000, recombinant enzyme, SDS-PAGE Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
3.1.26.4 ribonuclease HIII
-
 Geobacillus stearothermophilus
3.1.26.4 RNase HIII
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.4 70
-
 above Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.26.4 30 70 and above, temperature profile Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.26.4 55
-
 half-life 10 min Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.4 8.5 9
-
 Geobacillus stearothermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.26.4 Geobacillus stearothermophilus sequence calculation
-
 8.6