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Literature summary extracted from

  • Domingos, A.; Karmali, A.; Brown, P.R.
    One-step affinity purification of amidase from mutant strains of Pseudomonas aeruginosa (1989), Biochimie, 71, 1179-1184.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.4 32000
-
 strain B6, SDS-PAGE Pseudomonas aeruginosa
3.5.1.4 36000
-
 strain AI3, SDS-PAGE Pseudomonas aeruginosa
3.5.1.4 36000
-
 strain AIU1N, native PAGE Pseudomonas aeruginosa
3.5.1.4 36000
-
 strain AIU1N, SDS-PAGE Pseudomonas aeruginosa
3.5.1.4 36000
-
 strain L10, native PAGE Pseudomonas aeruginosa
3.5.1.4 36000
-
 strain L10, SDS-PAGE Pseudomonas aeruginosa
3.5.1.4 36000
-
 strain OUCH, native PAGE Pseudomonas aeruginosa
3.5.1.4 36000
-
 strain OUCH, SDS-PAGE Pseudomonas aeruginosa
3.5.1.4 125000
-
 strain B6, native PAGE Pseudomonas aeruginosa
3.5.1.4 148000
-
 strain AI3, native PAGE Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Pseudomonas aeruginosa
-
 mutant strains B6, AI3, AIU1N, OUCH and L10
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.4
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.4 0.89
-
 strain B6, substrate para-nitroacetanilide, pH 7.2, 25°C Pseudomonas aeruginosa
3.5.1.4 0.91
-
 strain L10, substrate para-nitroacetanilide, pH 7.2, 25°C Pseudomonas aeruginosa
3.5.1.4 2.35
-
 strain OUCH, substrate para-nitroacetanilide, pH 7.2, 25°C Pseudomonas aeruginosa
3.5.1.4 4.89
-
 strain AIU1N, substrate para-nitroacetanilide, pH 7.2, 25°C Pseudomonas aeruginosa
3.5.1.4 8.04
-
 strain AI3, substrate para-nitroacetanilide, pH 7.2, 25°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 acetamide + H2O
-
 Pseudomonas aeruginosa acetic acid + NH3
-
 ?
3.5.1.4 urea + H2O
-
 Pseudomonas aeruginosa carbamic acid + NH3
-
 ?