EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.70 | wild-type and mutant enzymes produced in Escherichia coli | Streptococcus mutans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.70 | W238A | mutant shows lower preference for kojibiose, nigerose, and sucrose and higher preference for maltooligosaccharides, trehalose, and p-nitrophenyl alpha-D-glucoside. Altough trehalose is not a substrate for the wild-type, the mutant enzyme hydrolyzes trehalose | Streptococcus mutans |
3.2.1.70 | W238N | mutant shows lower preference for kojibiose, nigerose, and sucrose and higher preference for maltooligosaccharides, trehalose, and p-nitrophenyl alpha-D-glucoside. Altough trehalose is not a substrate for the wild-type, the mutant enzyme hydrolyzes trehalose | Streptococcus mutans |
3.2.1.70 | W238P | mutant shows lower preference for kojibiose, nigerose, and sucrose and higher preference for maltooligosaccharides, trehalose, and p-nitrophenyl alpha-D-glucoside. Altough trehalose is not a substrate for the wild-type, the mutant enzyme hydrolyzes trehalose | Streptococcus mutans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.70 | 0.549 | - |
p-nitrophenyl alpha-D-glucoside | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
3.2.1.70 | 1.03 | - |
panose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
3.2.1.70 | 2.2 | - |
panose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.2.1.70 | 3.17 | - |
isomaltotriose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
3.2.1.70 | 4.22 | - |
panose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
3.2.1.70 | 4.96 | - |
isomaltotriose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.2.1.70 | 5.1 | - |
isomaltopentaose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
3.2.1.70 | 5.58 | - |
isomaltotetraose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
3.2.1.70 | 6.41 | - |
panose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
3.2.1.70 | 7.77 | - |
isomaltotetraose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.2.1.70 | 7.88 | - |
isomaltohexaose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
3.2.1.70 | 8.91 | - |
isomaltose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.2.1.70 | 9.35 | - |
isomaltose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
3.2.1.70 | 9.92 | - |
p-nitrophenyl alpha-D-glucoside | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.2.1.70 | 10.2 | - |
isomaltotriose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
3.2.1.70 | 11.4 | - |
isomaltotriose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
3.2.1.70 | 12 | - |
isomaltoheptaose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
3.2.1.70 | 15.1 | - |
isomaltopentaose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.2.1.70 | 15.8 | - |
isomaltose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
3.2.1.70 | 16.1 | - |
isomaltotetraose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
3.2.1.70 | 19 | - |
p-nitrophenyl alpha-D-glucoside | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
3.2.1.70 | 19.9 | - |
isomaltohexaose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.2.1.70 | 20.5 | - |
isomaltose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
3.2.1.70 | 20.9 | - |
isomaltotetraose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
3.2.1.70 | 24.6 | - |
isomaltopentaose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
3.2.1.70 | 26.9 | - |
isomaltoheptaose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.2.1.70 | 27.1 | - |
isomaltohexaose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
3.2.1.70 | 38.3 | - |
isomaltopentaose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
3.2.1.70 | 41.4 | - |
p-nitrophenyl alpha-D-glucoside | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
3.2.1.70 | 43.6 | - |
isomaltoheptaose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
3.2.1.70 | 76.9 | - |
isomaltohexaose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
3.2.1.70 | 81.7 | - |
isomaltoheptaose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.70 | 63000 | - |
wild-type enzyme, gel filtration | Streptococcus mutans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.70 | Streptococcus mutans | Q2HWU5 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.70 | 4-nitrophenyl alpha-D-glucoside + H2O | 377% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | 4-nitrophenol + D-glucose | - |
? | |
3.2.1.70 | dextran + H2O | - |
Streptococcus mutans | alpha-D-glucose | - |
? | |
3.2.1.70 | isomaltoheptaose + H2O | 148% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | D-glucose | - |
? | |
3.2.1.70 | isomaltohexaose + H2O | 148% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | D-glucose | - |
? | |
3.2.1.70 | isomaltopentaose + H2O | 148% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | D-glucose | - |
? | |
3.2.1.70 | isomaltose + H2O | - |
Streptococcus mutans | 2 D-glucose | - |
? | |
3.2.1.70 | isomaltotetraose + H2O | 148% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | D-glucose | - |
? | |
3.2.1.70 | isomaltotriose + H2O | 221% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | D-glucose | - |
? | |
3.2.1.70 | kojibiose + H2O | 4.02% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | 2 D-glucose | - |
? | |
3.2.1.70 | maltose + H2O | 0.0135% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | 2 D-glucose | - |
? | |
3.2.1.70 | maltotetraose + H2O | 0.0108% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | maltotriose + D-glucose | - |
? | |
3.2.1.70 | maltotriose + H2O | 0.146% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | maltose + D-glucose | - |
? | |
3.2.1.70 | additional information | no activity with trehalose | Streptococcus mutans | ? | - |
? | |
3.2.1.70 | nigerose + H2O | 1.99% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | 2 D-glucose | - |
? | |
3.2.1.70 | panose + H2O | 417% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | D-glucose | - |
? | |
3.2.1.70 | sucrose + H2O | 4.29% of the activity with isomaltose, wild-type enzyme | Streptococcus mutans | D-fructose + D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.70 | monomer | 1 * 63000, wild-type enzyme, SDS-PAGE | Streptococcus mutans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.70 | dextran glucosidase | - |
Streptococcus mutans |
3.2.1.70 | SMDG | - |
Streptococcus mutans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.70 | 40 | - |
15 min, pH 6.0, stable below | Streptococcus mutans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.70 | 0.04 | 1.97 | isomaltotetraose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.2.1.70 | 0.04 | 1.97 | isomaltopentaose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.2.1.70 | 3 | 6 | isomaltohexaose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
3.2.1.70 | 7.8 | - |
isomaltohexaose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.2.1.70 | 8.88 | - |
isomaltose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.2.1.70 | 8.96 | - |
isomaltotetraose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.2.1.70 | 9.21 | - |
isomaltopentaose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.2.1.70 | 9.81 | - |
isomaltoheptaose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.2.1.70 | 12.2 | - |
isomaltotriose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.2.1.70 | 22.3 | - |
isomaltotetraose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
3.2.1.70 | 22.4 | - |
panose | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.2.1.70 | 22.6 | - |
isomaltopentaose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
3.2.1.70 | 33.1 | - |
isomaltotriose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
3.2.1.70 | 33.2 | - |
isomaltoheptaose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
3.2.1.70 | 34.5 | - |
isomaltose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
3.2.1.70 | 39.6 | - |
isomaltohexaose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
3.2.1.70 | 42.1 | - |
panose | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
3.2.1.70 | 42.4 | - |
isomaltopentaose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
3.2.1.70 | 52.5 | - |
isomaltotetraose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
3.2.1.70 | 56.5 | - |
isomaltoheptaose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
3.2.1.70 | 61.4 | - |
p-nitrophenyl alpha-D-glucoside | pH 6.0, 37°C, mutant enzyme W238N | Streptococcus mutans | |
3.2.1.70 | 77.5 | - |
isomaltotriose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
3.2.1.70 | 79.8 | - |
isomaltose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
3.2.1.70 | 121 | - |
panose | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans | |
3.2.1.70 | 202 | - |
p-nitrophenyl alpha-D-glucoside | pH 6.0, 37°C, mutant enzyme W238A | Streptococcus mutans | |
3.2.1.70 | 235 | - |
p-nitrophenyl alpha-D-glucoside | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
3.2.1.70 | 401 | - |
isomaltohexaose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
3.2.1.70 | 419 | - |
isomaltopentaose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
3.2.1.70 | 449 | - |
isomaltoheptaose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
3.2.1.70 | 483 | - |
isomaltose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
3.2.1.70 | 499 | - |
isomaltotetraose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
3.2.1.70 | 538 | - |
panose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
3.2.1.70 | 541 | - |
isomaltotriose | pH 6.0, 37°C, wild-type enzyme | Streptococcus mutans | |
3.2.1.70 | 1202 | - |
p-nitrophenyl alpha-D-glucoside | pH 6.0, 37°C, mutant enzyme W238P | Streptococcus mutans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.70 | 6 | - |
- |
Streptococcus mutans |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.70 | 5.5 | 8.4 | 4°C, stable for 18 h | Streptococcus mutans |