BRENDA - Enzyme Database

Biochemical characterization of the class B acid phosphatase (AphA) of Escherichia coli MG1655

Passariello, C.; Forleo, C.; Micheli, V.; Schippa, S.; Leone, R.; Mangani, S.; Thaller, M.C.; Rossolini, G.M.; Biochim. Biophys. Acta 1764, 13-19 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.2
gene aphA, overexpression in strain DH5alpha
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.2
1,10-phenanthroline
IC50 is 0.0020 mM
Escherichia coli
3.1.3.2
dipicolinic acid
IC50 is 0.0025 mM
Escherichia coli
3.1.3.2
EDTA
IC50 is 0.0004 mM
Escherichia coli
3.1.3.2
EGTA
IC50 is 0.0025 mM
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.2
additional information
-
additional information
steady-state kinetics
Escherichia coli
3.1.3.2
0.0013
-
3'-dCMP
pH 6.0, 37°C, recombinant AphA
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.2
Escherichia coli
P0AE22
gene aphA
-
3.1.3.2
Escherichia coli MG1655
P0AE22
gene aphA
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.2
recombinant AphA from strain DH5alpha
Escherichia coli
Reaction
EC Number
Reaction
Commentary
Organism
3.1.3.2
a phosphate monoester + H2O = an alcohol + phosphate
modelling of substrate binding in the active site
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.2
additional information
-
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.2
3'-AMP + H2O
-
664223
Escherichia coli
adenosine + phosphate
-
-
-
?
3.1.3.2
3'-CMP + H2O
-
664223
Escherichia coli
cytidine + phosphate
-
-
-
?
3.1.3.2
3'-dAMP + H2O
-
664223
Escherichia coli
deoxyadenosine + phosphate
-
-
-
?
3.1.3.2
3'-dCMP + H2O
best substrate
664223
Escherichia coli
deoxycytidine + phosphate
-
-
-
?
3.1.3.2
3'-dGMP + H2O
-
664223
Escherichia coli
deoxyguanosine + phosphate
-
-
-
?
3.1.3.2
3'-dUMP + H2O
-
664223
Escherichia coli
deoxyuridine + phosphate
-
-
-
?
3.1.3.2
3'-GMP + H2O
-
664223
Escherichia coli
guanosine + phosphate
-
-
-
?
3.1.3.2
3'-UMP + H2O
-
664223
Escherichia coli
uridine + phosphate
-
-
-
?
3.1.3.2
3-phosphoglycerate + H2O
low activity
664223
Escherichia coli
glycerate + phosphate
-
-
-
?
3.1.3.2
4-nitrophenyl phosphate + H2O
-
664223
Escherichia coli
4-nitrophenol + phosphate
-
-
-
?
3.1.3.2
5'-AMP + H2O
-
664223
Escherichia coli
adenosine + phosphate
-
-
-
?
3.1.3.2
5'-CMP + H2O
-
664223
Escherichia coli
cytidine + phosphate
-
-
-
?
3.1.3.2
5'-dAMP + H2O
-
664223
Escherichia coli
deoxyadenosine + phosphate
-
-
-
?
3.1.3.2
5'-dCMP + H2O
-
664223
Escherichia coli
deoxycytidine + phosphate
-
-
-
?
3.1.3.2
5'-dGMP + H2O
-
664223
Escherichia coli
deoxyguanosine + phosphate
-
-
-
?
3.1.3.2
5'-dUMP + H2O
-
664223
Escherichia coli
deoxyuridine + phosphate
-
-
-
?
3.1.3.2
5'-GMP + H2O
-
664223
Escherichia coli
guanosine + phosphate
-
-
-
?
3.1.3.2
5'-UMP + H2O
-
664223
Escherichia coli
uridine + phosphate
-
-
-
?
3.1.3.2
ADP + H2O
-
664223
Escherichia coli
AMP + phosphate
-
-
-
?
3.1.3.2
ATP + H2O
-
664223
Escherichia coli
ADP + phosphate
-
-
-
?
3.1.3.2
D-glucose 6-phosphate + H2O
low activity
664223
Escherichia coli
D-glucose + phosphate
-
-
-
?
3.1.3.2
D-ribose 5-phosphate + H2O
low activity
664223
Escherichia coli
D-ribose + phosphate
-
-
-
?
3.1.3.2
additional information
substrate specificity of AphA, overview
664223
Escherichia coli
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.2
37
-
assay at
Escherichia coli
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.3.2
additional information
-
additional information
-
Escherichia coli
3.1.3.2
101
-
3'-dCMP
pH 6.0, 37°C, recombinant AphA
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.2
6
-
assay at
Escherichia coli
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.1.3.2
0.0004
-
IC50 is 0.0004 mM
Escherichia coli
EDTA
3.1.3.2
0.002
-
IC50 is 0.0020 mM
Escherichia coli
1,10-phenanthroline
3.1.3.2
0.0025
-
IC50 is 0.0025 mM
Escherichia coli
dipicolinic acid
3.1.3.2
0.0025
-
IC50 is 0.0025 mM
Escherichia coli
EGTA
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.2
gene aphA, overexpression in strain DH5alpha
Escherichia coli
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.1.3.2
0.0004
-
IC50 is 0.0004 mM
Escherichia coli
EDTA
3.1.3.2
0.002
-
IC50 is 0.0020 mM
Escherichia coli
1,10-phenanthroline
3.1.3.2
0.0025
-
IC50 is 0.0025 mM
Escherichia coli
dipicolinic acid
3.1.3.2
0.0025
-
IC50 is 0.0025 mM
Escherichia coli
EGTA
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.2
1,10-phenanthroline
IC50 is 0.0020 mM
Escherichia coli
3.1.3.2
dipicolinic acid
IC50 is 0.0025 mM
Escherichia coli
3.1.3.2
EDTA
IC50 is 0.0004 mM
Escherichia coli
3.1.3.2
EGTA
IC50 is 0.0025 mM
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.2
additional information
-
additional information
steady-state kinetics
Escherichia coli
3.1.3.2
0.0013
-
3'-dCMP
pH 6.0, 37°C, recombinant AphA
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.2
recombinant AphA from strain DH5alpha
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.2
additional information
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.2
3'-AMP + H2O
-
664223
Escherichia coli
adenosine + phosphate
-
-
-
?
3.1.3.2
3'-CMP + H2O
-
664223
Escherichia coli
cytidine + phosphate
-
-
-
?
3.1.3.2
3'-dAMP + H2O
-
664223
Escherichia coli
deoxyadenosine + phosphate
-
-
-
?
3.1.3.2
3'-dCMP + H2O
best substrate
664223
Escherichia coli
deoxycytidine + phosphate
-
-
-
?
3.1.3.2
3'-dGMP + H2O
-
664223
Escherichia coli
deoxyguanosine + phosphate
-
-
-
?
3.1.3.2
3'-dUMP + H2O
-
664223
Escherichia coli
deoxyuridine + phosphate
-
-
-
?
3.1.3.2
3'-GMP + H2O
-
664223
Escherichia coli
guanosine + phosphate
-
-
-
?
3.1.3.2
3'-UMP + H2O
-
664223
Escherichia coli
uridine + phosphate
-
-
-
?
3.1.3.2
3-phosphoglycerate + H2O
low activity
664223
Escherichia coli
glycerate + phosphate
-
-
-
?
3.1.3.2
4-nitrophenyl phosphate + H2O
-
664223
Escherichia coli
4-nitrophenol + phosphate
-
-
-
?
3.1.3.2
5'-AMP + H2O
-
664223
Escherichia coli
adenosine + phosphate
-
-
-
?
3.1.3.2
5'-CMP + H2O
-
664223
Escherichia coli
cytidine + phosphate
-
-
-
?
3.1.3.2
5'-dAMP + H2O
-
664223
Escherichia coli
deoxyadenosine + phosphate
-
-
-
?
3.1.3.2
5'-dCMP + H2O
-
664223
Escherichia coli
deoxycytidine + phosphate
-
-
-
?
3.1.3.2
5'-dGMP + H2O
-
664223
Escherichia coli
deoxyguanosine + phosphate
-
-
-
?
3.1.3.2
5'-dUMP + H2O
-
664223
Escherichia coli
deoxyuridine + phosphate
-
-
-
?
3.1.3.2
5'-GMP + H2O
-
664223
Escherichia coli
guanosine + phosphate
-
-
-
?
3.1.3.2
5'-UMP + H2O
-
664223
Escherichia coli
uridine + phosphate
-
-
-
?
3.1.3.2
ADP + H2O
-
664223
Escherichia coli
AMP + phosphate
-
-
-
?
3.1.3.2
ATP + H2O
-
664223
Escherichia coli
ADP + phosphate
-
-
-
?
3.1.3.2
D-glucose 6-phosphate + H2O
low activity
664223
Escherichia coli
D-glucose + phosphate
-
-
-
?
3.1.3.2
D-ribose 5-phosphate + H2O
low activity
664223
Escherichia coli
D-ribose + phosphate
-
-
-
?
3.1.3.2
additional information
substrate specificity of AphA, overview
664223
Escherichia coli
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.2
37
-
assay at
Escherichia coli
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.3.2
additional information
-
additional information
-
Escherichia coli
3.1.3.2
101
-
3'-dCMP
pH 6.0, 37°C, recombinant AphA
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.2
6
-
assay at
Escherichia coli