Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Passariello, C.; Forleo, C.; Micheli, V.; Schippa, S.; Leone, R.; Mangani, S.; Thaller, M.C.; Rossolini, G.M.
    Biochemical characterization of the class B acid phosphatase (AphA) of Escherichia coli MG1655 (2006), Biochim. Biophys. Acta, 1764, 13-19.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2 gene aphA, overexpression in strain DH5alpha Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 1,10-phenanthroline IC50 is 0.0020 mM Escherichia coli
3.1.3.2 dipicolinic acid IC50 is 0.0025 mM Escherichia coli
3.1.3.2 EDTA IC50 is 0.0004 mM Escherichia coli
3.1.3.2 EGTA IC50 is 0.0025 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 additional information
-
 additional information steady-state kinetics Escherichia coli
3.1.3.2 0.0013
-
 3'-dCMP pH 6.0, 37°C, recombinant AphA Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Escherichia coli P0AE22 gene aphA
-
3.1.3.2 Escherichia coli MG1655 P0AE22 gene aphA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 recombinant AphA from strain DH5alpha Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.2 a phosphate monoester + H2O = an alcohol + phosphate modelling of substrate binding in the active site Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 3'-AMP + H2O
-
 Escherichia coli adenosine + phosphate
-
 ?
3.1.3.2 3'-AMP + H2O
-
 Escherichia coli MG1655 adenosine + phosphate
-
 ?
3.1.3.2 3'-CMP + H2O
-
 Escherichia coli cytidine + phosphate
-
 ?
3.1.3.2 3'-dAMP + H2O
-
 Escherichia coli 3'-deoxyadenosine + phosphate
-
 ?
3.1.3.2 3'-dCMP + H2O best substrate Escherichia coli deoxycytidine + phosphate
-
 ?
3.1.3.2 3'-dGMP + H2O
-
 Escherichia coli deoxyguanosine + phosphate
-
 ?
3.1.3.2 3'-dUMP + H2O
-
 Escherichia coli deoxyuridine + phosphate
-
 ?
3.1.3.2 3'-GMP + H2O
-
 Escherichia coli guanosine + phosphate
-
 ?
3.1.3.2 3'-UMP + H2O
-
 Escherichia coli uridine + phosphate
-
 ?
3.1.3.2 3-phosphoglycerate + H2O low activity Escherichia coli glycerate + phosphate
-
 ?
3.1.3.2 3-phosphoglycerate + H2O low activity Escherichia coli MG1655 glycerate + phosphate
-
 ?
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Escherichia coli 4-nitrophenol + phosphate
-
 ?
3.1.3.2 5'-AMP + H2O
-
 Escherichia coli adenosine + phosphate
-
 ?
3.1.3.2 5'-AMP + H2O
-
 Escherichia coli MG1655 adenosine + phosphate
-
 ?
3.1.3.2 5'-CMP + H2O
-
 Escherichia coli cytidine + phosphate
-
 ?
3.1.3.2 5'-dAMP + H2O
-
 Escherichia coli deoxyadenosine + phosphate
-
 ?
3.1.3.2 5'-dCMP + H2O
-
 Escherichia coli deoxycytidine + phosphate
-
 ?
3.1.3.2 5'-dGMP + H2O
-
 Escherichia coli deoxyguanosine + phosphate
-
 ?
3.1.3.2 5'-dUMP + H2O
-
 Escherichia coli deoxyuridine + phosphate
-
 ?
3.1.3.2 5'-GMP + H2O
-
 Escherichia coli guanosine + phosphate
-
 ?
3.1.3.2 5'-UMP + H2O
-
 Escherichia coli uridine + phosphate
-
 ?
3.1.3.2 ADP + H2O
-
 Escherichia coli AMP + phosphate
-
 ?
3.1.3.2 ATP + H2O
-
 Escherichia coli ADP + phosphate
-
 ?
3.1.3.2 D-glucose 6-phosphate + H2O low activity Escherichia coli D-glucose + phosphate
-
 ?
3.1.3.2 D-ribose 5-phosphate + H2O low activity Escherichia coli D-ribose + phosphate
-
 ?
3.1.3.2 D-ribose 5-phosphate + H2O low activity Escherichia coli MG1655 D-ribose + phosphate
-
 ?
3.1.3.2 additional information substrate specificity of AphA, overview Escherichia coli ?
-
 ?
3.1.3.2 additional information substrate specificity of AphA, overview Escherichia coli MG1655 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 AphA
-
 Escherichia coli
3.1.3.2 More the enzyme belongs to the class B bacterial phosphatases as part of the DDDD superfamily of phosphohydrolases Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 37
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.2 additional information
-
 additional information
-
 Escherichia coli
3.1.3.2 101
-
 3'-dCMP pH 6.0, 37°C, recombinant AphA Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 6
-
 assay at Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.2 0.0004
-
 IC50 is 0.0004 mM Escherichia coli EDTA
3.1.3.2 0.002
-
 IC50 is 0.0020 mM Escherichia coli 1,10-phenanthroline
3.1.3.2 0.0025
-
 IC50 is 0.0025 mM Escherichia coli EGTA
3.1.3.2 0.0025
-
 IC50 is 0.0025 mM Escherichia coli dipicolinic acid