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Literature summary extracted from

  • Lucio-Eterovic, A.K.B.; Jorge, J.A.; Polizeli, M.de L.T.M.; Terenzi, H.F.
    Biochemical characterisation of the trehalase of thermophilic fungi: an enzyme with mixed properties of neutral and acid trehalase (2005), Biochim. Biophys. Acta, 1723, 201-207.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 1,10-phenanthroline inhibits the neutral trehalase in presence of 5 mM trehalose [Humicola] grisea var. thermoidea
3.2.1.28 1,10-phenanthroline inhibits the acid trehalase, inhibits the neutral trehalase in presence of 5 mM trehalose Mycothermus thermophilus
3.2.1.28 ATP
-
 [Humicola] grisea var. thermoidea
3.2.1.28 ATP
-
 Mycothermus thermophilus
3.2.1.28 ATP
-
 Thermochaetoides thermophila
3.2.1.28 EDTA
-
 [Humicola] grisea var. thermoidea
3.2.1.28 EDTA
-
 Mycothermus thermophilus
3.2.1.28 EDTA
-
 Thermochaetoides thermophila
3.2.1.28 gentiobiose slight inhibition of acid trehalase [Humicola] grisea var. thermoidea
3.2.1.28 gentiobiose slight inhibition of acid trehalase Mycothermus thermophilus
3.2.1.28 metal chelators
-
 [Humicola] grisea var. thermoidea
3.2.1.28 metal chelators
-
 Mycothermus thermophilus
3.2.1.28 metal chelators
-
 Thermochaetoides thermophila
3.2.1.28 methyl-alpha-glucoside slight inhibition of acid trehalase [Humicola] grisea var. thermoidea
3.2.1.28 methyl-alpha-glucoside slight inhibition of acid trehalase Mycothermus thermophilus
3.2.1.28 phloretin inhibits the acid trehalase, inhibits the neutral trehalase in presence of 20 mM trehalose [Humicola] grisea var. thermoidea
3.2.1.28 phloretin inhibits the acid trehalase, inhibits the neutral trehalase in presence of 20 mM trehalose Mycothermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 additional information
-
 additional information Km of neutral and acid trehalase at different temperatures and pH values Mycothermus thermophilus
3.2.1.28 additional information
-
 additional information Km of neutral and acid trehalase at different temperatures and pH values [Humicola] grisea var. thermoidea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.28 extracellular acid trehalase Thermochaetoides thermophila
-
-
3.2.1.28 extracellular acid trehalase Mycothermus thermophilus
-
-
3.2.1.28 extracellular acid trehalase [Humicola] grisea var. thermoidea
-
-
3.2.1.28 intracellular neutral trehalase Thermochaetoides thermophila 5622
-
3.2.1.28 intracellular neutral trehalase Mycothermus thermophilus 5622
-
3.2.1.28 intracellular neutral trehalase [Humicola] grisea var. thermoidea 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.28 Ca2+ activates Thermochaetoides thermophila
3.2.1.28 Ca2+ activates Mycothermus thermophilus
3.2.1.28 Ca2+ activates, dependent on pH [Humicola] grisea var. thermoidea
3.2.1.28 gentiobiose activates the neutral trehalase Mycothermus thermophilus
3.2.1.28 gentiobiose activates the neutral trehalase [Humicola] grisea var. thermoidea
3.2.1.28 Mn2+ activates Thermochaetoides thermophila
3.2.1.28 Mn2+ activates Mycothermus thermophilus
3.2.1.28 Mn2+ activates [Humicola] grisea var. thermoidea
3.2.1.28 phloretin activates the neutral trehalase in presence of 5 mM trehalose Mycothermus thermophilus
3.2.1.28 phloretin activates the neutral trehalase in presence of 5 mM trehalose [Humicola] grisea var. thermoidea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O Thermochaetoides thermophila
-
 2 D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O Mycothermus thermophilus
-
 2 D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O [Humicola] grisea var. thermoidea
-
 2 D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O Mycothermus thermophilus 77.7.8
-
 2 D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Mycothermus thermophilus
-
 isolated from indian soil
-
3.2.1.28 Mycothermus thermophilus 77.7.8
-
 isolated from indian soil
-
3.2.1.28 Thermochaetoides thermophila
-
-
-
3.2.1.28 [Humicola] grisea var. thermoidea
-
 isolated from brazilian soil
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.28 glycoprotein acid trehalase Thermochaetoides thermophila
3.2.1.28 glycoprotein acid trehalase Mycothermus thermophilus
3.2.1.28 glycoprotein acid trehalase [Humicola] grisea var. thermoidea

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28
-
 Mycothermus thermophilus
3.2.1.28
-
 [Humicola] grisea var. thermoidea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.28 additional information
-
 activities of neutral and acid trehalase at different pH values and temperatures Mycothermus thermophilus
3.2.1.28 additional information
-
 activities of neutral and acid trehalase at different pH values and temperatures [Humicola] grisea var. thermoidea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Thermochaetoides thermophila 2 D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Mycothermus thermophilus 2 D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O
-
 [Humicola] grisea var. thermoidea 2 D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Mycothermus thermophilus 77.7.8 2 D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.28 acid trehalase
-
 Thermochaetoides thermophila
3.2.1.28 acid trehalase
-
 Mycothermus thermophilus
3.2.1.28 acid trehalase
-
 [Humicola] grisea var. thermoidea
3.2.1.28 neutral trehalase
-
 Thermochaetoides thermophila
3.2.1.28 neutral trehalase
-
 Mycothermus thermophilus
3.2.1.28 neutral trehalase
-
 [Humicola] grisea var. thermoidea
3.2.1.28 trehalase
-
 Thermochaetoides thermophila
3.2.1.28 trehalase
-
 Mycothermus thermophilus
3.2.1.28 trehalase
-
 [Humicola] grisea var. thermoidea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.28 additional information
-
 the acid trehalase shows a higher temperature optimum than the neutral trehalase Thermochaetoides thermophila
3.2.1.28 60
-
 the acid trehalase shows a higher temperature optimum than the neutral trehalase Mycothermus thermophilus
3.2.1.28 60
-
 the acid trehalase shows a higher temperature optimum than the neutral trehalase [Humicola] grisea var. thermoidea

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.28 30 60
-
 [Humicola] grisea var. thermoidea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.28 additional information
-
 the acid trehalase is more thermostable than the neutral trehalase Thermochaetoides thermophila
3.2.1.28 additional information
-
 the acid trehalase is more thermostable than the neutral trehalase Mycothermus thermophilus
3.2.1.28 additional information
-
 the acid trehalase is more thermostable than the neutral trehalase [Humicola] grisea var. thermoidea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 4.5
-
 acid trehalase Mycothermus thermophilus
3.2.1.28 4.5
-
 acid trehalase [Humicola] grisea var. thermoidea
3.2.1.28 6.5
-
 neutral trehalase Mycothermus thermophilus
3.2.1.28 6.5
-
 neutral trehalase [Humicola] grisea var. thermoidea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.28 4.5 7
-
 Mycothermus thermophilus
3.2.1.28 4.5 7
-
 [Humicola] grisea var. thermoidea