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Literature summary extracted from

  • Jaeger, K.E.; Adrian, F.J.; Meyer, H.E.; Hancock, R.E.W.; Winkler, U.K.
    Extracellular lipase from Pseudomonas aeruginosa is an amphiphilic protein (1992), Biochim. Biophys. Acta, 1120, 315-321.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 diethyl 4-nitrophenyl phosphate rapid and complete inhibition Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular the enzyme is secreted Pseudomonas aeruginosa
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Pseudomonas aeruginosa
-
-
-
3.1.1.3 Pseudomonas aeruginosa PAC1R
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 extracellular enzyme by chromatographical isoelectric focusing Pseudomonas aeruginosa

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.3 triacylglycerol + H2O = diacylglycerol + a carboxylate the enzyme contains an active site serine residue Pseudomonas aeruginosa
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Pseudomonas aeruginosa 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Pseudomonas aeruginosa PAC1R 4-nitrophenol + palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 More the enzyme contains the conserved N-terminal consensus sequence KYP-IVL-VHG, sodium deoxycholate and cetyltrimethylammonium bromide shift the electrophoretic mobility of the enzyme in anodal or cathodal direction, respectively, amphiphilic enzyme Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 extracellular lipase
-
 Pseudomonas aeruginosa
3.1.1.3 lipase
-
 Pseudomonas aeruginosa
3.1.1.3 triacylglycerol acylhydrolase
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 20
-
 assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 6
-
 assay at Pseudomonas aeruginosa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.3 Pseudomonas aeruginosa isoelectric focusing
-
 5.7