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Literature summary extracted from

  • Shibuya, Y.; Yamamoto, T.; Morimoto, T.; Nishino, N.; Kambara, T.; Okabe, H.
    Pseudomonas aeruginosa alkaline proteinase might share a biological function with plasmin (1991), Biochim. Biophys. Acta, 1077, 316-324.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.24.40 medicine anti-coagulant activity in human plasma Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.40 1,10-phenanthroline pH 7.8, 37°C, 1 mM 66% inhibition, 3mM 90% inhibition Pseudomonas aeruginosa
3.4.24.40 Ac-Val-Leu-Lys-4-mercaptoanilide pH 7.8, 37°C, 0.2 mM 69% inhibition, 0.3 mM complete inhibition Pseudomonas aeruginosa
3.4.24.40 additional information not inhibited by chloromethylketone or hydroxamate Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.40 0.021
-
 t-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide pH 7.8, 37°C Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.40 Zn2+
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.40 57000
-
 SDS-PAGE Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.40 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.40 HPLC Pseudomonas aeruginosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.40 Fibrin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Fibrinogen + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Pro-Phe-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Suc-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Leu-Ser-Thr-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Met-Thr-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Phe-Ser-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas aeruginosa ?
-
 ?