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Literature summary extracted from

  • Turan, Y.; Zheng, M.
    Purification and characterization of an intracellular beta-glucosidase from the methylotrophic yeast Pichia pastoris (2005), Biochemistry (Moscow), 70, 1363-1368.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 2-nitrophenyl-beta-D-fucopyranoside competitively versus substrate 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris
3.2.1.21 4-nitrophenyl-beta-D-fucopyranoside competitively versus substrate 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris
3.2.1.21 D-glucose competitively versus substrate 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris
3.2.1.21 gluconolactone competitively versus substrate 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.096
-
 4-methylumbelliferyl beta-D-glucoside pH 7.3, 40°C Komagataella pastoris
3.2.1.21 0.12
-
 4-nitrophenyl beta-D-glucopyranoside pH 7.3, 40°C Komagataella pastoris
3.2.1.21 0.22
-
 2-nitrophenyl beta-D-glucopyranoside pH 7.3, 40°C Komagataella pastoris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 intracellular
-
 Komagataella pastoris 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 70000
-
 4 * 70000, SDS-PAGE Komagataella pastoris
3.2.1.21 275000
-
 gel filtration Komagataella pastoris

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Komagataella pastoris
-
-
-
3.2.1.21 Komagataella pastoris X-33
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity by ammonium sulfate fractionation, gel filtration, ion exchange chromatography, and adsorption chromatography Komagataella pastoris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 11
-
 purified enzyme Komagataella pastoris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 2-nitrophenyl beta-D-glucopyranoside + H2O 131% of the activity with 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 2-nitrophenyl beta-D-glucopyranoside + H2O 131% of the activity with 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris X-33 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 4-methylumbelliferyl beta-D-glucoside + H2O 63% of the activity with 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris 4-methylumbelliferol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Komagataella pastoris 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl-1-thio-beta-D-glucopyranoside + H2O 31% of the activity with 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris 4-nitrophenol + 1-thio-beta-D-glucose
-
 ?
3.2.1.21 amygdalin + H2O 11.3% of the activity with 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris ?
-
 ?
3.2.1.21 amygdalin + H2O 11.3% of the activity with 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris X-33 ?
-
 ?
3.2.1.21 beta-gentiobiose + H2O 6.2% of the activity with 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris 2 D-glucose
-
 ?
3.2.1.21 D-(+)-cellobiose + H2O 12.9% of the activity with 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris beta-D-glucose
-
 ?
3.2.1.21 linamarin + H2O 34% of the activity with 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris alpha-hydroxyisobutyronitrile + D-glucose
-
 ?
3.2.1.21 additional information no activity with arbutin Komagataella pastoris ?
-
 ?
3.2.1.21 additional information no activity with arbutin Komagataella pastoris X-33 ?
-
 ?
3.2.1.21 n-dodecyl-beta-D-glucopyranoside + H2O 26% of the activity with 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris n-dodecanol + D-Glucose
-
 ?
3.2.1.21 n-octyl-beta-D-glucopyranoside + H2O 28% of the activity with 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris n-octanol + D-glucose
-
 ?
3.2.1.21 n-octyl-beta-D-glucopyranoside + H2O 28% of the activity with 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris X-33 n-octanol + D-glucose
-
 ?
3.2.1.21 prunasin + H2O 27% of the activity with 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris D-mandelonitrile + beta-D-glucose
-
 ?
3.2.1.21 prunasin + H2O 27% of the activity with 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris X-33 D-mandelonitrile + beta-D-glucose
-
 ?
3.2.1.21 salicin + H2O 10.4% of the activity with 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris saligenin + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 tetramer 4 * 70000, SDS-PAGE Komagataella pastoris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 40
-
-
 Komagataella pastoris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 7.2
-
 4-methylumbelliferyl beta-D-glucoside pH 7.3, 40°C Komagataella pastoris
3.2.1.21 11.6
-
 4-nitrophenyl beta-D-glucopyranoside pH 7.3, 40°C Komagataella pastoris
3.2.1.21 13.7
-
 2-nitrophenyl beta-D-glucopyranoside pH 7.3, 40°C Komagataella pastoris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 7.3
-
-
 Komagataella pastoris

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 5.5 9.5 fairly stable Komagataella pastoris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.21 0.41
-
 D-glucose pH 7.3, 40°C, versus substrate 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris
3.2.1.21 0.41
-
 2-nitrophenyl-beta-D-fucopyranoside pH 7.3, 40°C, versus substrate 4-nitrophenyl beta-D-glucopyranoside Komagataella pastoris