EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.21 | 2-nitrophenyl-beta-D-fucopyranoside | competitively versus substrate 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | |
3.2.1.21 | 4-nitrophenyl-beta-D-fucopyranoside | competitively versus substrate 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | |
3.2.1.21 | D-glucose | competitively versus substrate 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | |
3.2.1.21 | gluconolactone | competitively versus substrate 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 0.096 | - |
4-methylumbelliferyl beta-D-glucoside | pH 7.3, 40°C | Komagataella pastoris | |
3.2.1.21 | 0.12 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 7.3, 40°C | Komagataella pastoris | |
3.2.1.21 | 0.22 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 7.3, 40°C | Komagataella pastoris |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.21 | intracellular | - |
Komagataella pastoris | 5622 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 70000 | - |
4 * 70000, SDS-PAGE | Komagataella pastoris |
3.2.1.21 | 275000 | - |
gel filtration | Komagataella pastoris |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.21 | Komagataella pastoris | - |
- |
- |
3.2.1.21 | Komagataella pastoris X-33 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.21 | to homogeneity by ammonium sulfate fractionation, gel filtration, ion exchange chromatography, and adsorption chromatography | Komagataella pastoris |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 11 | - |
purified enzyme | Komagataella pastoris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | 2-nitrophenyl beta-D-glucopyranoside + H2O | 131% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | 2-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.21 | 2-nitrophenyl beta-D-glucopyranoside + H2O | 131% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris X-33 | 2-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.21 | 4-methylumbelliferyl beta-D-glucoside + H2O | 63% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | 4-methylumbelliferol + beta-D-glucose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Komagataella pastoris | 4-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.21 | 4-nitrophenyl-1-thio-beta-D-glucopyranoside + H2O | 31% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | 4-nitrophenol + 1-thio-beta-D-glucose | - |
? | |
3.2.1.21 | amygdalin + H2O | 11.3% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | ? | - |
? | |
3.2.1.21 | amygdalin + H2O | 11.3% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris X-33 | ? | - |
? | |
3.2.1.21 | beta-gentiobiose + H2O | 6.2% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | 2 D-glucose | - |
? | |
3.2.1.21 | D-(+)-cellobiose + H2O | 12.9% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | beta-D-glucose | - |
? | |
3.2.1.21 | linamarin + H2O | 34% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | alpha-hydroxyisobutyronitrile + D-glucose | - |
? | |
3.2.1.21 | additional information | no activity with arbutin | Komagataella pastoris | ? | - |
? | |
3.2.1.21 | additional information | no activity with arbutin | Komagataella pastoris X-33 | ? | - |
? | |
3.2.1.21 | n-dodecyl-beta-D-glucopyranoside + H2O | 26% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | n-dodecanol + D-Glucose | - |
? | |
3.2.1.21 | n-octyl-beta-D-glucopyranoside + H2O | 28% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | n-octanol + D-glucose | - |
? | |
3.2.1.21 | n-octyl-beta-D-glucopyranoside + H2O | 28% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris X-33 | n-octanol + D-glucose | - |
? | |
3.2.1.21 | prunasin + H2O | 27% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | D-mandelonitrile + beta-D-glucose | - |
? | |
3.2.1.21 | prunasin + H2O | 27% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris X-33 | D-mandelonitrile + beta-D-glucose | - |
? | |
3.2.1.21 | salicin + H2O | 10.4% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | saligenin + D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.21 | tetramer | 4 * 70000, SDS-PAGE | Komagataella pastoris |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 40 | - |
- |
Komagataella pastoris |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 7.2 | - |
4-methylumbelliferyl beta-D-glucoside | pH 7.3, 40°C | Komagataella pastoris | |
3.2.1.21 | 11.6 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 7.3, 40°C | Komagataella pastoris | |
3.2.1.21 | 13.7 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 7.3, 40°C | Komagataella pastoris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 7.3 | - |
- |
Komagataella pastoris |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 5.5 | 9.5 | fairly stable | Komagataella pastoris |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 0.41 | - |
D-glucose | pH 7.3, 40°C, versus substrate 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | |
3.2.1.21 | 0.41 | - |
2-nitrophenyl-beta-D-fucopyranoside | pH 7.3, 40°C, versus substrate 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris |