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Literature summary extracted from

  • Marti-Arbona, R.; Xu, C.; Steele, S.; Weeks, A.; Kuty, G.F.; Seibert, C.M.; Raushel, F.M.
    Annotating enzymes of unknown function: N-formimino-L-glutamate deiminase is a member of the amidohydrolase superfamily (2006), Biochemistry, 45, 1997-2005.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.13 expression in Escherichia coli Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.13 additional information no inhibition by N-acetyl-L-glutamate, N-carbamoyl-L-glutamate, N-formyl-L-glutamate, N-formimino-alpha-aminobutyric acid and N-formimino-gamma-aminobutyric acid at concentrations up to 10 mM Pseudomonas aeruginosa
3.5.3.13 N-formimino-L-aspartate
-
 Pseudomonas aeruginosa
3.5.3.13 N-guanidino-L-glutamate
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.13 0.0012
-
 N-formimino-L-glutamate Zn2+ has been reconstituted with Cu2+, pH 8.0, 30°C Pseudomonas aeruginosa
3.5.3.13 0.0021
-
 N-formimino-L-glutamate Zn2+ has been reconstituted with Cd2+, pH 8.0, 30°C Pseudomonas aeruginosa
3.5.3.13 0.0027
-
 N-formimino-L-glutamate Zn2+ has been reconstituted with Ni2+, pH 8.0, 30°C Pseudomonas aeruginosa
3.5.3.13 0.22
-
 N-formimino-L-glutamate wild-type, pH 8.0, 30°C Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.13 Zn2+ bound to the protein Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.13 49000
-
 2 * 49000, SDS-PAGE, gel filtration Pseudomonas aeruginosa
3.5.3.13 100000
-
 gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.13 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.13
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.13 N-formimino-alpha-aminobutyric acid + H2O pH 8.0, 30°C Pseudomonas aeruginosa N-formyl-alpha-aminobutyric acid + NH3
-
 ?
3.5.3.13 N-formimino-D-glutamate + H2O pH 8.0, 30°C Pseudomonas aeruginosa N-formyl-D-glutamate + NH3
-
 ?
3.5.3.13 N-formimino-gamma-aminobutyric acid + H2O pH 8.0, 30°C Pseudomonas aeruginosa N-formyl-gamma-aminobutyric acid + NH3
-
 ?
3.5.3.13 N-formimino-glycine + H2O pH 8.0, 30°C Pseudomonas aeruginosa N-formyl-glycine + NH3
-
 ?
3.5.3.13 N-formimino-L-alanine + H2O pH 8.0, 30°C Pseudomonas aeruginosa N-formyl-L-alanine + NH3
-
 ?
3.5.3.13 N-formimino-L-aspartate + H2O pH 8.0, 30°C Pseudomonas aeruginosa N-formyl-L-aspartate + NH3
-
 ?
3.5.3.13 N-formimino-L-glutamate + H2O pH 8.0, 30°C Pseudomonas aeruginosa N-formyl-L-glutamate + NH3
-
 ?
3.5.3.13 N-formimino-L-isoleucine + H2O pH 8.0, 30°C Pseudomonas aeruginosa N-formyl-L-isoleucine + NH3
-
 ?
3.5.3.13 N-formimino-L-leucine + H2O pH 8.0, 30°C Pseudomonas aeruginosa N-formyl-L-leucine + NH3
-
 ?
3.5.3.13 N-formimino-L-methionine + H2O pH 8.0, 30°C Pseudomonas aeruginosa N-formyl-L-methionine + NH3
-
 ?
3.5.3.13 N-formimino-L-phenylalanine + H2O pH 8.0, 30°C Pseudomonas aeruginosa N-formyl-L-phenylalanine + NH3
-
 ?
3.5.3.13 N-formimino-L-tyrosine + H2O pH 8.0, 30°C Pseudomonas aeruginosa N-formyl-L-tyrosine + NH3
-
 ?
3.5.3.13 N-formimino-L-valine + H2O pH 8.0, 30°C Pseudomonas aeruginosa N-formyl-L-valine + NH3
-
 ?
3.5.3.13 N-guanidino-L-glutamate + H2O pH 8.0, 30°C Pseudomonas aeruginosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.13 dimer 2 * 49000, SDS-PAGE, gel filtration Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.5.3.13 N-formimino-L-glutamate deiminase
-
 Pseudomonas aeruginosa
3.5.3.13 Pa5106
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.13 additional information
-
 additional information less than 0.001/sec for the substrates N-guanidino-L-glutamate, N-formimino-alpha-aminobutyric acid, N-formimino-gamma-aminobutyric acid, N-formimino-L-alanine, N-formimino-L-aspartate, N-formimino-glycine, N-formimino-L-leucine, N-formimino-L-phenylalanine, N-formimino-L-tyrosine, N-formimino-L-valine Pseudomonas aeruginosa
3.5.3.13 0.01
-
 N-formimino-L-methionine pH 8.0, 30°C Pseudomonas aeruginosa
3.5.3.13 0.019
-
 N-formimino-L-isoleucine pH 8.0, 30°C Pseudomonas aeruginosa
3.5.3.13 0.05
-
 N-formimino-D-glutamate pH 8.0, 30°C Pseudomonas aeruginosa
3.5.3.13 8.5
-
 N-formimino-L-glutamate Zn2+ has been reconstituted with Cu2+, pH 8.0, 30°C Pseudomonas aeruginosa
3.5.3.13 13.2
-
 N-formimino-L-glutamate pH 8.0, 30°C Pseudomonas aeruginosa
3.5.3.13 21.3
-
 N-formimino-L-glutamate Zn2+ has been reconstituted with Ni2+, pH 8.0, 30°C Pseudomonas aeruginosa
3.5.3.13 31
-
 N-formimino-L-glutamate Zn2+ has been reconstituted with Cd2+, pH 8.0, 30°C Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.3.13 0.03
-
 N-formimino-L-aspartate pH 8.0, 30°C Pseudomonas aeruginosa
3.5.3.13 0.89
-
 N-guanidino-L-glutamate pH 8.0, 30°C Pseudomonas aeruginosa