EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.3.6 | expression in Escherichia coli | Pseudomonas aeruginosa |
3.5.3.6 | expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Pseudomonas aeruginosa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.3.6 | C406A | inactive mutant enzyme | Pseudomonas aeruginosa |
3.5.3.6 | C406A | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
3.5.3.6 | C406S | inactive mutant enzyme | Pseudomonas aeruginosa |
3.5.3.6 | C406S | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
3.5.3.6 | D166A | inactive mutant enzyme | Pseudomonas aeruginosa |
3.5.3.6 | D166A | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
3.5.3.6 | D280A | inactive mutant enzyme | Pseudomonas aeruginosa |
3.5.3.6 | D280A | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
3.5.3.6 | E224A | kcat/Km is 23684fold higher than the wild-type value | Pseudomonas aeruginosa |
3.5.3.6 | E224A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
3.5.3.6 | E224D | kcat/Km is 50000fold higher than the wild-type value | Pseudomonas aeruginosa |
3.5.3.6 | E224D | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
3.5.3.6 | H278A | inactive mutant enzyme | Pseudomonas aeruginosa |
3.5.3.6 | H278A | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
3.5.3.6 | H278N | inactive mutant enzyme | Pseudomonas aeruginosa |
3.5.3.6 | H278N | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
3.5.3.6 | H278V | inactive mutant enzyme | Pseudomonas aeruginosa |
3.5.3.6 | H278V | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
3.5.3.6 | N160A | kcat/Km is 5625fold higher than the wild-type value | Pseudomonas aeruginosa |
3.5.3.6 | N160A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
3.5.3.6 | R185A | kcat/Km is 7627fold higher than the wild-type value | Pseudomonas aeruginosa |
3.5.3.6 | R185A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
3.5.3.6 | R185K | site-directed mutagenesis, unstable mutant enzyme that precipitates | Pseudomonas aeruginosa |
3.5.3.6 | R185L | kcat/Km is 25000fold higher than the wild-type value | Pseudomonas aeruginosa |
3.5.3.6 | R185L | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
3.5.3.6 | R243A | kcat/Km is 150000fold higher than the wild-type value | Pseudomonas aeruginosa |
3.5.3.6 | R243A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
3.5.3.6 | R243K | kcat/Km is 150000fold higher than the wild-type value | Pseudomonas aeruginosa |
3.5.3.6 | R243K | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
3.5.3.6 | R243L | kcat/Km is 225000fold higher than the wild-type value | Pseudomonas aeruginosa |
3.5.3.6 | R243L | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
3.5.3.6 | R401A | kcat/Km is 56250fold higher than the wild-type value | Pseudomonas aeruginosa |
3.5.3.6 | R401A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
3.5.3.6 | R401L | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.3.6 | additional information | - |
additional information | steady-state kinetics | Pseudomonas aeruginosa | |
3.5.3.6 | 0.14 | - |
L-arginine | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
3.5.3.6 | 0.14 | - |
L-Arg | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
3.5.3.6 | 1.1 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme E224A | Pseudomonas aeruginosa | |
3.5.3.6 | 1.1 | - |
L-arginine | pH 5.6, 25°C, mutant E224A | Pseudomonas aeruginosa | |
3.5.3.6 | 2.6 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R243A | Pseudomonas aeruginosa | |
3.5.3.6 | 2.6 | - |
L-arginine | pH 5.6, 25°C, mutant R243A | Pseudomonas aeruginosa | |
3.5.3.6 | 2.7 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme N160A | Pseudomonas aeruginosa | |
3.5.3.6 | 2.7 | - |
L-arginine | pH 5.6, 25°C, mutant N160A | Pseudomonas aeruginosa | |
3.5.3.6 | 4.2 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme E224D | Pseudomonas aeruginosa | |
3.5.3.6 | 4.2 | - |
L-arginine | pH 5.6, 25°C, mutant E224D | Pseudomonas aeruginosa | |
3.5.3.6 | 4.8 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R185L | Pseudomonas aeruginosa | |
3.5.3.6 | 4.8 | - |
L-arginine | pH 5.6, 25°C, mutant R185L | Pseudomonas aeruginosa | |
3.5.3.6 | 6.9 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R243K | Pseudomonas aeruginosa | |
3.5.3.6 | 6.9 | - |
L-arginine | pH 5.6, 25°C, mutant R243K | Pseudomonas aeruginosa | |
3.5.3.6 | 8.7 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R401A | Pseudomonas aeruginosa | |
3.5.3.6 | 8.7 | - |
L-arginine | pH 5.6, 25°C, mutant R401A | Pseudomonas aeruginosa | |
3.5.3.6 | 15 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R185A | Pseudomonas aeruginosa | |
3.5.3.6 | 15 | - |
L-arginine | pH 5.6, 25°C, mutant R185A | Pseudomonas aeruginosa | |
3.5.3.6 | 21 | - |
Nomega-methyl-Arg | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
3.5.3.6 | 21 | - |
Nomega-methyl-L-arginine | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
3.5.3.6 | 38 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R243L | Pseudomonas aeruginosa | |
3.5.3.6 | 38 | - |
L-arginine | pH 5.6, 25°C, mutant R243L | Pseudomonas aeruginosa | |
3.5.3.6 | 50 | - |
Nomega-amino-L-arginine | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
3.5.3.6 | 50 | - |
Nomega-amino-Arg | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.3.6 | KCl | increases the activity 6fold at 60 mM | Pseudomonas aeruginosa | |
3.5.3.6 | MgCl2 | increases the activity 8fold at 20 mM | Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.3.6 | L-arginine + H2O | Pseudomonas aeruginosa | ADI catalyzes the first step of the energy-producing arginine degradation pathway found in certain microorganisms, which ultimately leads to ornithine, carbamate, and ATP, overview | L-citrulline + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.3.6 | Pseudomonas aeruginosa | - |
- |
- |
3.5.3.6 | Pseudomonas aeruginosa | P13981 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.3.6 | recombinant wild-type and mutant enzyme | Pseudomonas aeruginosa |
3.5.3.6 | recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) | Pseudomonas aeruginosa |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.5.3.6 | L-arginine + H2O = L-citrulline + NH3 | residues Cys406, His278, and Asp166 and the contribution from the neighboring Asp280 are important in catalysis of the formation and hydrolysis of the Cys406-alkyluronium intermediate, structure and kinetic analysis | Pseudomonas aeruginosa |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.3.6 | additional information | - |
- |
Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.3.6 | L-Arg + H2O | - |
Pseudomonas aeruginosa | L-citrulline + NH3 | - |
? | |
3.5.3.6 | L-arginine + H2O | specific for | Pseudomonas aeruginosa | L-citrulline + NH3 | - |
? | |
3.5.3.6 | L-arginine + H2O | ADI catalyzes the first step of the energy-producing arginine degradation pathway found in certain microorganisms, which ultimately leads to ornithine, carbamate, and ATP, overview | Pseudomonas aeruginosa | L-citrulline + NH3 | - |
? | |
3.5.3.6 | additional information | no activity with agmatine | Pseudomonas aeruginosa | ? | - |
? | |
3.5.3.6 | Nomega-amino-Arg + H2O | - |
Pseudomonas aeruginosa | L-citrulline + hydrazine | - |
? | |
3.5.3.6 | Nomega-amino-L-arginine + H2O | - |
Pseudomonas aeruginosa | L-citrulline + hydrazine | - |
? | |
3.5.3.6 | Nomega-methyl-Arg + H2O | - |
Pseudomonas aeruginosa | L-citrulline + methylamine | - |
? | |
3.5.3.6 | Nomega-methyl-L-arginine + H2O | - |
Pseudomonas aeruginosa | L-citrulline + methylamine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.3.6 | More | ADI C406A-L-arginine structure, overview | Pseudomonas aeruginosa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.3.6 | ADI | - |
Pseudomonas aeruginosa |
3.5.3.6 | More | ADI belongs to the guanidino group-modifying enzyme superfamily, GMSF, which conserves backbone fold and a Cys-, His-, and Asp-based catalytic core | Pseudomonas aeruginosa |
3.5.3.6 | PaADI | - |
Pseudomonas aeruginosa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.3.6 | 25 | - |
assay at | Pseudomonas aeruginosa |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.3.6 | additional information | - |
no activity at 45°C | Pseudomonas aeruginosa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.3.6 | 0.00036 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R401L | Pseudomonas aeruginosa | |
3.5.3.6 | 0.00089 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R243A | Pseudomonas aeruginosa | |
3.5.3.6 | 0.002 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R243K | Pseudomonas aeruginosa | |
3.5.3.6 | 0.0021 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme E224A | Pseudomonas aeruginosa | |
3.5.3.6 | 0.0037 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme E224D | Pseudomonas aeruginosa | |
3.5.3.6 | 0.0072 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R401A | Pseudomonas aeruginosa | |
3.5.3.6 | 0.0084 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R185L | Pseudomonas aeruginosa | |
3.5.3.6 | 0.009 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R243L | Pseudomonas aeruginosa | |
3.5.3.6 | 0.01 | - |
Nomega-methyl-Arg | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
3.5.3.6 | 0.021 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme N160A | Pseudomonas aeruginosa | |
3.5.3.6 | 0.089 | - |
L-Arg | pH 5.6, 25°C, mutant enzyme R185A | Pseudomonas aeruginosa | |
3.5.3.6 | 0.2 | - |
L-arginine | pH 5.6, 25°C, mutant R243L | Pseudomonas aeruginosa | |
3.5.3.6 | 0.3 | - |
L-arginine | pH 5.6, 25°C, mutant R243A | Pseudomonas aeruginosa | |
3.5.3.6 | 0.3 | - |
L-arginine | pH 5.6, 25°C, mutant R243K | Pseudomonas aeruginosa | |
3.5.3.6 | 0.8 | - |
L-arginine | pH 5.6, 25°C, mutant R401A | Pseudomonas aeruginosa | |
3.5.3.6 | 0.9 | - |
L-arginine | pH 5.6, 25°C, mutant E224D | Pseudomonas aeruginosa | |
3.5.3.6 | 1 | - |
Nomega-methyl-L-arginine | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
3.5.3.6 | 1.8 | - |
L-arginine | pH 5.6, 25°C, mutant R185L | Pseudomonas aeruginosa | |
3.5.3.6 | 1.9 | - |
L-arginine | pH 5.6, 25°C, mutant E224A | Pseudomonas aeruginosa | |
3.5.3.6 | 5.9 | - |
L-arginine | pH 5.6, 25°C, mutant R185A | Pseudomonas aeruginosa | |
3.5.3.6 | 6.3 | - |
L-arginine | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
3.5.3.6 | 6.3 | - |
L-Arg | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
3.5.3.6 | 7 | - |
Nomega-amino-L-arginine | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
3.5.3.6 | 7 | - |
Nomega-amino-Arg | pH 5.6, 25°C, wild-type enzyme | Pseudomonas aeruginosa | |
3.5.3.6 | 8 | - |
L-arginine | pH 5.6, 25°C, mutant N160A | Pseudomonas aeruginosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.3.6 | 5.6 | - |
assay at | Pseudomonas aeruginosa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.3.6 | 5 | 8.6 | pH profiles of wild-type and mutant enzymes | Pseudomonas aeruginosa |