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Literature summary extracted from

  • Shallom, D.; Leon, M.; Bravman, T.; Ben-David, A.; Zaide, G.; Belakhov, V.; Shoham, G.; Schomburg, D.; Baasov, T.; Shoham, Y.
    Biochemical characterization and identification of the catalytic residues of a family 43 beta-D-xylosidase from Geobacillus stearothermophilus T-6 (2005), Biochemistry, 44, 387-397.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.37 gene xynB3, expression of soluble wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Geobacillus stearothermophilus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.37 D128G site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme, nearly inactive mutant Geobacillus stearothermophilus
3.2.1.37 D15G site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme, nearly inactive mutant Geobacillus stearothermophilus
3.2.1.37 E178G site-directed mutagenesis, the mutant shows an altered substrate specificity and highly reduced activity compared to the wild-type enzyme Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 Ag+ 97% inhibition at 1 mM Geobacillus stearothermophilus
3.2.1.37 Hg2+ complete inhibition at 1 mM, 93% inhibition at 0.1 mM Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 additional information
-
 additional information steady-state kinetics, recombinant wild-type and mutant enzymes Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.37 additional information the enzyme is not affected by 1 mM of Fe3+, Ca2+, Co2+, Mg2+, Ba2+, Ni2+, K+, Zn2+, Cu2+, or EDTA Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 180000
-
 gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Geobacillus stearothermophilus Q09LX0 i.e. Bacillus stearothermophilus, strain T-6, gene xynB3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 recombinant soluble wild-type, 2.6fold to over 95% purity, and mutant enzymes from Escherichia coli strain BL21(DE3) by heat treatment and gel filtration Geobacillus stearothermophilus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.37 (Xylbeta(1-4))x + H2O = beta-D-xylopyranose + (Xylbeta(1-4))x-1 catalytic residues D15, D128, and E187 are essential for activity, reaction mechanism Geobacillus stearothermophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 2,5-dinitrophenyl-beta-D-xylopyranoside + H2O
-
 Geobacillus stearothermophilus 2,5-dinitrophenol + beta-D-xylose
-
 ?
3.2.1.37 3,4-dinitrophenyl beta-D-xylopyranoside + H2O
-
 Geobacillus stearothermophilus 3,4-dinitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.37 3,5-dinitrophenyl beta-D-xylopyranoside + H2O
-
 Geobacillus stearothermophilus 3,5-dinitrophenol + beta-D-xylose
-
 ?
3.2.1.37 4-bromophenyl-beta-D-xylopyranoside + H2O low activity of mutant E178G, no activity of the wild-type enzyme Geobacillus stearothermophilus 4-bromophenol + beta-D-xylose
-
 ?
3.2.1.37 4-methylumbelliferyl-beta-D-xylopyranoside + H2O
-
 Geobacillus stearothermophilus 4-methylumbelliferol + beta-D-xylose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Geobacillus stearothermophilus 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 additional information substrate specificity of wild-type enzyme and mutant E178G, the mutant enzyme shows residual activity with 2-naphthyl-beta-D-xylopyranoside, 3,4-dimethylphenyl-beta-D-xylopyranoside, and 3-nitrophenyl-beta-D-xylopyranoside, while the wild-type enzyme is inactive with these substrates, overview Geobacillus stearothermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 trimer in solution Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 beta-D-xylosidase
-
 Geobacillus stearothermophilus
3.2.1.37 More the enzyme belongs to the glycosyl hydrolase family 43, GH43 Geobacillus stearothermophilus
3.2.1.37 XynB3
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 65
-
-
 Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.37 additional information
-
 temperature profile and thermal stability Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 additional information
-
 temperature profile and thermal stability Geobacillus stearothermophilus
3.2.1.37 60
-
 fully stable up to Geobacillus stearothermophilus
3.2.1.37 75
-
 inactivation Geobacillus stearothermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.37 0.014
-
 4-nitrophenyl-beta-D-xylopyranoside pH 7.0, 40°C, recombinant mutant E178G Geobacillus stearothermophilus
3.2.1.37 1.8
-
 3,4-dinitrophenyl-beta-D-xylopyranoside pH 7.0, 40°C, recombinant mutant E178G Geobacillus stearothermophilus
3.2.1.37 20
-
 4-methylumbelliferyl-beta-D-xylopyranoside pH 7.0, 40°C, recombinant wild-type enzyme Geobacillus stearothermophilus
3.2.1.37 25
-
 2,5-dinitrophenyl-beta-D-xylopyranoside pH 7.0, 40°C, recombinant wild-type enzyme Geobacillus stearothermophilus
3.2.1.37 30
-
 2,5-dinitrophenyl-beta-D-xylopyranoside pH 7.0, 40°C, recombinant mutant E178G Geobacillus stearothermophilus
3.2.1.37 57
-
 4-nitrophenyl-beta-D-xylopyranoside pH 7.0, 40°C, recombinant wild-type enzyme Geobacillus stearothermophilus
3.2.1.37 77
-
 3,4-dinitrophenyl-beta-D-xylopyranoside pH 7.0, 40°C, recombinant wild-type enzyme Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 5
-
-
 Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.37 additional information
-
 pH profiles, wild-type and mutant enzymes, overview Geobacillus stearothermophilus