EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.3.3.7 | crystal structure of mutant enzyme R138H and R138A, hanging-drop vapor diffusion method | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.3.3.7 | R138A | activity is approximately 0.1% of wild-type activity, Km-value for L-aspartate 4-semialdehyde is significantly higher than the wild-type value, shows the same IC50 values as the wild-type enzyme, but different partial inhibition patterns | Escherichia coli |
4.3.3.7 | R138H | activity is approximately 0.1% of wild-type activity, Km-value for L-aspartate 4-semialdehyde is significantly higher than the wild-type value, shows the same IC50 values as the wild-type enzyme, but different partial inhibition patterns | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.3.3.7 | (S)-lysine | mutant enzymes R138H and R138A show the same IC50 values as the wild-type enzyme, but different partial inhibition patterns | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.3.7 | 0.28 | - |
pyruvate | mutant enzyme R138H | Escherichia coli | |
4.3.3.7 | 0.45 | - |
pyruvate | mutant enzyme R138A | Escherichia coli | |
4.3.3.7 | 5.1 | - |
L-aspartate 4-semialdehyde | mutant enzyme R138A | Escherichia coli | |
4.3.3.7 | 37 | - |
L-aspartate 4-semialdehyde | mutant enzyme R138H | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.3.3.7 | 31000 | - |
x * 31000, SDS-PAGE | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.3.7 | L-aspartate 4-semialdehyde + pyruvate | Escherichia coli | - |
dihydrodipicolinate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.3.7 | Escherichia coli | P0A6L2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.3.7 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.3.7 | L-aspartate 4-semialdehyde + pyruvate | - |
Escherichia coli | dihydrodipicolinate + H2O | - |
? | |
4.3.3.7 | L-aspartate 4-semialdehyde + pyruvate | branch poit of (S)-lysine biosynthesis | Escherichia coli | dihydrodipicolinate + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.3.3.7 | ? | x * 31000, SDS-PAGE | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.3.3.7 | DHDPS | - |
Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.3.7 | 0.038 | - |
pyruvate | mutant enzyme R138H | Escherichia coli | |
4.3.3.7 | 0.038 | - |
L-aspartate 4-semialdehyde | mutant enzyme R138H | Escherichia coli | |
4.3.3.7 | 0.149 | - |
pyruvate | mutant enzyme R138A | Escherichia coli | |
4.3.3.7 | 0.149 | - |
L-aspartate 4-semialdehyde | mutant enzyme R138A | Escherichia coli |