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Literature summary extracted from

  • Sukdeo, N.; Clugston, S.L.; Daub, E.; Honek, J.F.
    Distinct classes of glyoxalase I: metal specificity of the Yersinia pestis, Pseudomonas aeruginosa and Neisseria meningitidis enzymes (2004), Biochem. J., 384, 111-117.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.5 expression in Escherichia coli Pseudomonas aeruginosa
4.4.1.5 expression in Escherichia coli Neisseria meningitidis
4.4.1.5 expression in Escherichia coli Yersinia pestis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.5 0.016
-
 (R)-S-lactoylglutathione pH 7.0, 0.5 mM CoCl2 Pseudomonas aeruginosa
4.4.1.5 0.016
-
 methylglyoxal presence of Co2+, pH 7.0 Pseudomonas aeruginosa
4.4.1.5 0.028
-
 (R)-S-lactoylglutathione pH 7.0, 0.5 mM CoCl2 Neisseria meningitidis
4.4.1.5 0.028
-
 methylglyoxal presence of Co2+, pH 7.0 Neisseria meningitidis
4.4.1.5 0.029
-
 (R)-S-lactoylglutathione pH 7.0, 0.5 mM CoCl2 Yersinia pestis
4.4.1.5 0.029
-
 methylglyoxal presence of Co2+, pH 7.0 Yersinia pestis
4.4.1.5 0.032
-
 (R)-S-lactoylglutathione pH 7.0, 0.5 mM NiCl2 Pseudomonas aeruginosa
4.4.1.5 0.032
-
 methylglyoxal presence of Ni2+, pH 7.0 Pseudomonas aeruginosa
4.4.1.5 0.045
-
 (R)-S-lactoylglutathione pH 7.0, 0.5 mM NiCl2 Neisseria meningitidis
4.4.1.5 0.045
-
 methylglyoxal presence of Ni2+, pH 7.0 Neisseria meningitidis
4.4.1.5 0.056
-
 (R)-S-lactoylglutathione pH 7.0, 0.5 mM NiCl2 Yersinia pestis
4.4.1.5 0.056
-
 methylglyoxal presence of Ni2+, pH 7.0 Yersinia pestis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.5 Cd2+ in decreasing order of activation: Ni2+, Co2+, Cd2+, Mn2+, Zn2+ Neisseria meningitidis
4.4.1.5 Cd2+ in decreasing order of activation: Ni2+, Co2+, Mn2+, Cd2+ Pseudomonas aeruginosa
4.4.1.5 Cd2+ in decreasing order of activation: Ni2+, Co2+, Mn2+, Cd2+ Yersinia pestis
4.4.1.5 Cd2+ increased enzyme activity Pseudomonas aeruginosa
4.4.1.5 Cd2+ increased enzyme activity Neisseria meningitidis
4.4.1.5 Cd2+ increased enzyme activity Yersinia pestis
4.4.1.5 Co2+ in decreasing order of activation: Ni2+, Co2+, Cd2+, Mn2+, Zn2+ Neisseria meningitidis
4.4.1.5 Co2+ in decreasing order of activation: Ni2+, Co2+, Mn2+, Cd2+ Pseudomonas aeruginosa
4.4.1.5 Co2+ in decreasing order of activation: Ni2+, Co2+, Mn2+, Cd2+ Yersinia pestis
4.4.1.5 Co2+ increased enzyme activity Pseudomonas aeruginosa
4.4.1.5 Co2+ increased enzyme activity Neisseria meningitidis
4.4.1.5 Co2+ increased enzyme activity Yersinia pestis
4.4.1.5 Mn2+ in decreasing order of activation: Ni2+, Co2+, Cd2+, Mn2+, Zn2+ Neisseria meningitidis
4.4.1.5 Mn2+ in decreasing order of activation: Ni2+, Co2+, Mn2+, Cd2+ Pseudomonas aeruginosa
4.4.1.5 Mn2+ in decreasing order of activation: Ni2+, Co2+, Mn2+, Cd2+ Yersinia pestis
4.4.1.5 Mn2+ increased enzyme activity Pseudomonas aeruginosa
4.4.1.5 Mn2+ increased enzyme activity Neisseria meningitidis
4.4.1.5 Mn2+ increased enzyme activity Yersinia pestis
4.4.1.5 additional information Mg2+, Ca2+, Zn2+ no increase in enzyme activity Pseudomonas aeruginosa
4.4.1.5 additional information Mg2+, Ca2+, Zn2+ no increase in enzyme activity Neisseria meningitidis
4.4.1.5 additional information Mg2+, Ca2+, Zn2+ no increase in enzyme activity Yersinia pestis
4.4.1.5 Ni2+ in decreasing order of activation: Ni2+, Co2+, Cd2+, Mn2+, Zn2+ Neisseria meningitidis
4.4.1.5 Ni2+ in decreasing order of activation: Ni2+, Co2+, Mn2+, Cd2+ Pseudomonas aeruginosa
4.4.1.5 Ni2+ in decreasing order of activation: Ni2+, Co2+, Mn2+, Cd2+ Yersinia pestis
4.4.1.5 Ni2+ increased enzyme activity Pseudomonas aeruginosa
4.4.1.5 Ni2+ increased enzyme activity Neisseria meningitidis
4.4.1.5 Ni2+ increased enzyme activity Yersinia pestis
4.4.1.5 Zn2+ in decreasing order of activation: Ni2+, Co2+, Cd2+, Mn2+, Zn2+ Neisseria meningitidis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.5 14250
-
 electrospray ionization mass spectrometry Pseudomonas aeruginosa
4.4.1.5 14251
-
 x * 14251, electrospray ionization mass spectrometry, x * 14251, calculated Pseudomonas aeruginosa
4.4.1.5 14833
-
 x * 14833, electrospray ionization mass spectrometry, x * 14834, calculated Yersinia pestis
4.4.1.5 14834
-
 x * 14833, electrospray ionization mass spectrometry, x * 14834, calculated Yersinia pestis
4.4.1.5 15669
-
 x * 15669, electrospray ionization mass spectrometry, x * 15669, calculated Neisseria meningitidis
4.4.1.5 15670
-
 electrospray ionization mass spectrometry Neisseria meningitidis
4.4.1.5 29670
-
 electrospray ionization mass spectrometry Yersinia pestis
4.4.1.5 148333
-
 2 * 148333, electrospray ionization mass spectrometry Yersinia pestis

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.5 Neisseria meningitidis
-
-
-
4.4.1.5 Pseudomonas aeruginosa
-
-
-
4.4.1.5 Yersinia pestis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.5
-
 Pseudomonas aeruginosa
4.4.1.5
-
 Neisseria meningitidis
4.4.1.5
-
 Yersinia pestis
4.4.1.5 recombinant enzyme Pseudomonas aeruginosa
4.4.1.5 recombinant enzyme Neisseria meningitidis
4.4.1.5 recombinant enzyme Yersinia pestis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.5 140
-
 presence of Co2+, pH 7.0 Yersinia pestis
4.4.1.5 180
-
 presence of Co2+, pH 7.0 Pseudomonas aeruginosa
4.4.1.5 279
-
 presence of Co2+, pH 7.0 Neisseria meningitidis
4.4.1.5 390
-
 micromol/min/mg protein, Vmax (R)-S-lactoylglutathione, pH 7.0, 0.5 mM NiCl2, 279 micromol/min/mg protein Vmax (R)-S-lactoylglutathione, pH 7.0, 0.5 mM CoCl2 Neisseria meningitidis
4.4.1.5 390
-
 presence of Ni2+, pH 7.0 Neisseria meningitidis
4.4.1.5 571
-
 micromol/min/mg protein, Vmax (R)-S-lactoylglutathione, pH 7.0, 0.5 mM NiCl2, 180 micromol/min/mg protein Vmax (R)-S-lactoylglutathione, pH 7.0, 0.5 mM CoCl2 Pseudomonas aeruginosa
4.4.1.5 571
-
 presence of Ni2+, pH 7.0 Pseudomonas aeruginosa
4.4.1.5 618
-
 micromol/min/mg protein, Vmax (R)-S-lactoylglutathione, pH 7.0, 0.5 mM NiCl2, 140 micromol/min/mg protein Vmax (R)-S-lactoylglutathione, pH 7.0, 0.5 mM CoCl2 Yersinia pestis
4.4.1.5 618
-
 presence of Ni2+, pH 7.0 Yersinia pestis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.5 (R)-S-lactoylglutathione
-
 Pseudomonas aeruginosa glutathione + methylglyoxal
-
 ?
4.4.1.5 (R)-S-lactoylglutathione
-
 Neisseria meningitidis glutathione + methylglyoxal
-
 ?
4.4.1.5 (R)-S-lactoylglutathione
-
 Yersinia pestis glutathione + methylglyoxal
-
 ?
4.4.1.5 glutathione + methylglyoxal
-
 Pseudomonas aeruginosa S-lactoylglutathione
-
 ?
4.4.1.5 glutathione + methylglyoxal
-
 Neisseria meningitidis S-lactoylglutathione
-
 ?
4.4.1.5 glutathione + methylglyoxal
-
 Yersinia pestis S-lactoylglutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.5 ? x * 14251, electrospray ionization mass spectrometry, x * 14251, calculated Pseudomonas aeruginosa
4.4.1.5 ? x * 14833, electrospray ionization mass spectrometry, x * 14834, calculated Yersinia pestis
4.4.1.5 ? x * 15669, electrospray ionization mass spectrometry, x * 15669, calculated Neisseria meningitidis
4.4.1.5 dimer 2 * 148333, electrospray ionization mass spectrometry Yersinia pestis

Synonyms

EC Number Synonyms Comment Organism
4.4.1.5 GLXI
-
 Pseudomonas aeruginosa
4.4.1.5 GLXI
-
 Neisseria meningitidis
4.4.1.5 GLXI
-
 Yersinia pestis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.5 69
-
 methylglyoxal presence of Co2+, pH 7.0 Yersinia pestis
4.4.1.5 86
-
 methylglyoxal presence of Co2+, pH 7.0 Pseudomonas aeruginosa
4.4.1.5 146
-
 methylglyoxal presence of Co2+, pH 7.0 Neisseria meningitidis
4.4.1.5 204
-
 methylglyoxal presence of Ni2+, pH 7.0 Neisseria meningitidis
4.4.1.5 271
-
 methylglyoxal presence of Ni2+, pH 7.0 Pseudomonas aeruginosa
4.4.1.5 306
-
 methylglyoxal presence of Ni2+, pH 7.0 Yersinia pestis