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Literature summary extracted from

  • Lima, V.M.G.; Krieger, N.; Mitchell, D.A.; Fontana, J.D.
    Activity and stability of a crude lipase from Penicillium aurantiogriseum in aqueous media and organic solvents (2004), Biochem. Eng. J., 18, 65-71.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 n-heptane 13.6% activation at pH 8.0 Penicillium aurantiogriseum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 BaCl2 30% inhibition at 1 mM Penicillium aurantiogriseum
3.1.1.3 CaCl2 21% inhibition at 1 mM Penicillium aurantiogriseum
3.1.1.3 CuSO4 31% inhibition at 1 mM Penicillium aurantiogriseum
3.1.1.3 HgCl2 complete inhibition at 1 mM Penicillium aurantiogriseum
3.1.1.3 KCN 62% inhibition at 1 mM Penicillium aurantiogriseum
3.1.1.3 Triton X-100 48% inhibition at 0.01% Penicillium aurantiogriseum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 CoCl2 5% activation at 1 mM Penicillium aurantiogriseum
3.1.1.3 EDTA 18% activation at 1 mM, 9% at 10 mM Penicillium aurantiogriseum
3.1.1.3 MgCl2 13% activation at 1 mM Penicillium aurantiogriseum
3.1.1.3 MnCl2 28% activation at 1 mM Penicillium aurantiogriseum
3.1.1.3 additional information no effect by SDS at 0.01% Penicillium aurantiogriseum
3.1.1.3 NaN3 30% activation at 1 mM Penicillium aurantiogriseum
3.1.1.3 Tween 80 9% activation at 0.01% Penicillium aurantiogriseum
3.1.1.3 ZnCl2 12% activation at 1 mM Penicillium aurantiogriseum

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 Acetone complete inactivation at pH 8.0 Penicillium aurantiogriseum
3.1.1.3 Butanol 93.6% inactivation at pH 8.0 Penicillium aurantiogriseum
3.1.1.3 Ethanol complete inactivation at pH 8.0 Penicillium aurantiogriseum
3.1.1.3 hexane 8% inactivation at pH 8.0 Penicillium aurantiogriseum
3.1.1.3 isooctane 9% inactivation at pH 8.0 Penicillium aurantiogriseum
3.1.1.3 isopropanol 98% inactivation at pH 8.0 Penicillium aurantiogriseum
3.1.1.3 Methanol complete inactivation at pH 8.0 Penicillium aurantiogriseum
3.1.1.3 n-heptane 13.6% activation at pH 8.0 Penicillium aurantiogriseum
3.1.1.3 toluene 17.7% inactivation at pH 8.0 Penicillium aurantiogriseum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Penicillium aurantiogriseum
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 3.2
-
 crude enzyme extract, substrate triolein, activity depends on pH and buffer system used, high activity at pH 8.0 in citrate-phosphate or Tris-HCl buffer Penicillium aurantiogriseum
3.1.1.3 18
-
 crude enzyme extract, substrate tributyrin, activity depends on pH and buffer system used, high activity at pH 8.0 in citrate-phosphate or Tris-HCl buffer Penicillium aurantiogriseum
3.1.1.3 47
-
 crude enzyme extract, substrate 4-nitrophenyl butyrate, activity depends on pH and buffer system used, high activity at pH 8.0 in citrate-phosphate or Tris-HCl buffer Penicillium aurantiogriseum
3.1.1.3 74
-
 crude enzyme extract, substrate 4-nitrophenyl caproate, activity depends on pH and buffer system used, high activity at pH 8.0 in citrate-phosphate or Tris-HCl buffer Penicillium aurantiogriseum
3.1.1.3 88
-
 crude enzyme extract, substrate 4-nitrophenyl palmitate, activity depends on pH and buffer system used, high activity at pH 8.0 in citrate-phosphate or Tris-HCl buffer Penicillium aurantiogriseum
3.1.1.3 94
-
 crude enzyme extract, substrate 4-nitrophenyl caprate, activity depends on pH and buffer system used, high activity at pH 8.0 in citrate-phosphate or Tris-HCl buffer Penicillium aurantiogriseum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Penicillium aurantiogriseum 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl caprate + H2O best substrate Penicillium aurantiogriseum 4-nitrophenol + caprate
-
 ?
3.1.1.3 4-nitrophenyl caproate + H2O
-
 Penicillium aurantiogriseum 4-nitrophenol + caproate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Penicillium aurantiogriseum 4-nitrophenol + palmitate
-
 ?
3.1.1.3 additional information no activity with 4-nitrophenyl acetate Penicillium aurantiogriseum ?
-
 ?
3.1.1.3 tributyrin + H2O
-
 Penicillium aurantiogriseum dibutyrin + butyrate
-
 ?
3.1.1.3 triolein + H2O
-
 Penicillium aurantiogriseum diolein + oleate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 60
-
 phosphate buffer, pH 8.0 Penicillium aurantiogriseum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 30 70
-
 Penicillium aurantiogriseum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 additional information
-
 parameters of a two-step denaturing model, overview Penicillium aurantiogriseum
3.1.1.3 28
-
 preincubation increases activity in crude extract by 20% within 120 min Penicillium aurantiogriseum
3.1.1.3 37
-
 half-life 120 min, 77% remaining activity after 30 min Penicillium aurantiogriseum
3.1.1.3 45
-
 half-life 10 min, 45% remaining activity after 30 min, 30% remaining activity after 60 min Penicillium aurantiogriseum
3.1.1.3 50
-
 half-life below 5 min, 32% remaining activity after 30 min Penicillium aurantiogriseum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 7 8 depends on the buffer system used Penicillium aurantiogriseum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 5 9 over 80% remaining activity after preincubation at 28°C for 1 h, at pH 6.0-8.0 the preincubation increases activity in crude extract Penicillium aurantiogriseum