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Literature summary extracted from

  • Gu, X.; Chen, M.; Shen, H.; Jiang, X.; Huang, Y.; Wang, H.
    Rv2131c gene product: an unconventional enzyme that is both inositol monophosphatase and fructose-1,6-bisphosphatase (2006), Biochem. Biophys. Res. Commun., 339, 897-904.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.25 gene Rv2131c, expression of soluble N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.11 D40N almost complete loss of both inositol monophosphatase and fructose 1,6-bisphosphatase activity Mycobacterium tuberculosis
3.1.3.11 D94N almost complete loss of both inositol monophosphatase and fructose 1,6-bisphosphatase activity Mycobacterium tuberculosis
3.1.3.11 L71A no loss of either of the activities, activity toward inositol is more resisitant to inhibition by calcium than wild-type Mycobacterium tuberculosis
3.1.3.25 D40N site-sirected mutagenesis, nearly inactive mutant concerning both inositol monophosphatase and fructose-1,6-bisphosphatase activities Mycobacterium tuberculosis
3.1.3.25 D94N site-sirected mutagenesis, nearly inactive mutant concerning both inositol monophosphatase and fructose-1,6-bisphosphatase activities Mycobacterium tuberculosis
3.1.3.25 L71A site-sirected mutagenesis, no decrease in catalytic activity, but the mutant enzyme is 12fold more resistant to inhibition by Li+ compared to the wild-type enzyme Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.25 Li+
-
Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 0.45
-
fructose 1,6-bisphosphate 37°C, pH 10.0 Mycobacterium tuberculosis
3.1.3.25 0.22
-
D-myo-Inositol 1-phosphate pH 9.3, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.25 Mg2+ dependent on Mycobacterium tuberculosis
3.1.3.25 Mn2+ can partially substitute for Mg2+ Mycobacterium tuberculosis
3.1.3.25 additional information no activity wth Co2+, Ni2+, Cu2+, Zn2+, and Ca2+, no effect by Na+ and K+ Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.25 32000
-
2 * 32000, about, recombinant wild-type enzyme, SDS-PAGE Mycobacterium tuberculosis
3.1.3.25 62500
-
native PAGE, recombinant wild-type enzyme Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Mycobacterium tuberculosis
-
recombinant enzyme, shows dual activity of both inositol monophosphatase and fructose 1,6-bisphosphatase
-
3.1.3.25 Mycobacterium tuberculosis
-
gene Rv2131c
-
3.1.3.25 Mycobacterium tuberculosis H37Rv
-
gene Rv2131c
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.25 recombinant N-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Mycobacterium tuberculosis

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.25 myo-inositol phosphate + H2O = myo-inositol + phosphate enzyme is both inositol monophosphatase and fructose-1,6-bisphosphatase Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
Mycobacterium tuberculosis D-fructose 6-phosphate + phosphate
-
?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
Mycobacterium tuberculosis H37Rv D-fructose 6-phosphate + phosphate
-
?
3.1.3.25 D-myo-inositol 1-phosphate + H2O
-
Mycobacterium tuberculosis D-myo-inositol + phosphate
-
?
3.1.3.25 D-myo-inositol 1-phosphate + H2O
-
Mycobacterium tuberculosis H37Rv D-myo-inositol + phosphate
-
?
3.1.3.25 additional information substrate specificity, enzyme is both inositol monophosphatase and fructose-1,6-bisphosphatase Mycobacterium tuberculosis ?
-
?
3.1.3.25 additional information substrate specificity, enzyme is both inositol monophosphatase and fructose-1,6-bisphosphatase Mycobacterium tuberculosis H37Rv ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.25 dimer 2 * 32000, about, recombinant wild-type enzyme, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.1.3.25 IMPase
-
Mycobacterium tuberculosis
3.1.3.25 IMPase/FBPase
-
Mycobacterium tuberculosis
3.1.3.25 inositol monophosphatase
-
Mycobacterium tuberculosis
3.1.3.25 inositol monophosphatase/fructose-1,6-bisphosphatase
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.25 62
-
-
Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.25 70
-
2 min, loss of 95% activity Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.25 9.3
-
assay at Mycobacterium tuberculosis