EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.25 | gene Rv2131c, expression of soluble N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.11 | D40N | almost complete loss of both inositol monophosphatase and fructose 1,6-bisphosphatase activity | Mycobacterium tuberculosis |
3.1.3.11 | D94N | almost complete loss of both inositol monophosphatase and fructose 1,6-bisphosphatase activity | Mycobacterium tuberculosis |
3.1.3.11 | L71A | no loss of either of the activities, activity toward inositol is more resisitant to inhibition by calcium than wild-type | Mycobacterium tuberculosis |
3.1.3.25 | D40N | site-sirected mutagenesis, nearly inactive mutant concerning both inositol monophosphatase and fructose-1,6-bisphosphatase activities | Mycobacterium tuberculosis |
3.1.3.25 | D94N | site-sirected mutagenesis, nearly inactive mutant concerning both inositol monophosphatase and fructose-1,6-bisphosphatase activities | Mycobacterium tuberculosis |
3.1.3.25 | L71A | site-sirected mutagenesis, no decrease in catalytic activity, but the mutant enzyme is 12fold more resistant to inhibition by Li+ compared to the wild-type enzyme | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.25 | Li+ | - |
Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.11 | 0.45 | - |
fructose 1,6-bisphosphate | 37°C, pH 10.0 | Mycobacterium tuberculosis | |
3.1.3.25 | 0.22 | - |
D-myo-Inositol 1-phosphate | pH 9.3, 37°C, recombinant wild-type enzyme | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.25 | Mg2+ | dependent on | Mycobacterium tuberculosis | |
3.1.3.25 | Mn2+ | can partially substitute for Mg2+ | Mycobacterium tuberculosis | |
3.1.3.25 | additional information | no activity wth Co2+, Ni2+, Cu2+, Zn2+, and Ca2+, no effect by Na+ and K+ | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.25 | 32000 | - |
2 * 32000, about, recombinant wild-type enzyme, SDS-PAGE | Mycobacterium tuberculosis |
3.1.3.25 | 62500 | - |
native PAGE, recombinant wild-type enzyme | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.11 | Mycobacterium tuberculosis | - |
recombinant enzyme, shows dual activity of both inositol monophosphatase and fructose 1,6-bisphosphatase | - |
3.1.3.25 | Mycobacterium tuberculosis | - |
gene Rv2131c | - |
3.1.3.25 | Mycobacterium tuberculosis H37Rv | - |
gene Rv2131c | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.25 | recombinant N-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mycobacterium tuberculosis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.3.25 | myo-inositol phosphate + H2O = myo-inositol + phosphate | enzyme is both inositol monophosphatase and fructose-1,6-bisphosphatase | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.11 | D-fructose 1,6-bisphosphate + H2O | - |
Mycobacterium tuberculosis | D-fructose 6-phosphate + phosphate | - |
? | |
3.1.3.11 | D-fructose 1,6-bisphosphate + H2O | - |
Mycobacterium tuberculosis H37Rv | D-fructose 6-phosphate + phosphate | - |
? | |
3.1.3.25 | D-myo-inositol 1-phosphate + H2O | - |
Mycobacterium tuberculosis | D-myo-inositol + phosphate | - |
? | |
3.1.3.25 | D-myo-inositol 1-phosphate + H2O | - |
Mycobacterium tuberculosis H37Rv | D-myo-inositol + phosphate | - |
? | |
3.1.3.25 | additional information | substrate specificity, enzyme is both inositol monophosphatase and fructose-1,6-bisphosphatase | Mycobacterium tuberculosis | ? | - |
? | |
3.1.3.25 | additional information | substrate specificity, enzyme is both inositol monophosphatase and fructose-1,6-bisphosphatase | Mycobacterium tuberculosis H37Rv | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.25 | dimer | 2 * 32000, about, recombinant wild-type enzyme, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.25 | IMPase | - |
Mycobacterium tuberculosis |
3.1.3.25 | IMPase/FBPase | - |
Mycobacterium tuberculosis |
3.1.3.25 | inositol monophosphatase | - |
Mycobacterium tuberculosis |
3.1.3.25 | inositol monophosphatase/fructose-1,6-bisphosphatase | - |
Mycobacterium tuberculosis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.25 | 62 | - |
- |
Mycobacterium tuberculosis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.25 | 70 | - |
2 min, loss of 95% activity | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.25 | 9.3 | - |
assay at | Mycobacterium tuberculosis |