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Literature summary extracted from

  • Kaczowka, S.J.; Reuter, C.J.; Talarico, L.A.; Maupin-Furlow, J.A.
    Recombinant production of Zymomonas mobilis pyruvate decarboxylase in the haloarchaeon Haloferax volcanii (2005), Archaea, 1, 327-334.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.2.4.1 biotechnology active expression of enzyme from non-halophilic Zymomonas mobilis in the haloarchaeon Haloferax volcanii with no difference in the secondary structure. Post-transcriptional mechanisms in the stationary phase appear to limit the amount of recombinant protein expressed Zymomonas mobilis

General Stability

EC Number General Stability Organism
4.1.1.1 retains about 50% of activity in buffer supplemented with 2 M NaCl or KCl, retain 20% of activity in 4 M salt buffer Zymomonas mobilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.4.1 KCl at 2 M, 50% residual activity of enzyme expressed in Escherichia coli Zymomonas mobilis
1.2.4.1 NaCl at 2 M, 50% residual activity of enzyme expressed in Escherichia coli Zymomonas mobilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.4.1 60000
-
 4 * 60000, SDS-PAGE Zymomonas mobilis
1.2.4.1 240000
-
 gel filtration, recombinant protein from Escherichia coli and from Haloferax volcanii Zymomonas mobilis
4.1.1.1 60000
-
 4 * 60000, SDS-PAGE Zymomonas mobilis
4.1.1.1 240000
-
 gel filtration Zymomonas mobilis

Organism

EC Number Organism UniProt Comment Textmining
1.2.4.1 Zymomonas mobilis
-
 recombinant expression in Haloferax volcanii
-
4.1.1.1 Zymomonas mobilis
-
 expression in Haloferax volcanii, growth-phase dependent decrease in amount of enzyme protein
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.4.1 recombinant enzyme both expressed in Escherichia coli and Haloferax volcanii Zymomonas mobilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.4.1 55
-
 recombinant enzyme from Haloferax volcanii Zymomonas mobilis
1.2.4.1 91
-
 recombinant enzyme from Escherichia coli Zymomonas mobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.4.1 pyruvate + CoA + NAD+
-
 Zymomonas mobilis acetyl-CoA + CO2 + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.4.1 tetramer 4 * 60000, SDS-PAGE Zymomonas mobilis
4.1.1.1 tetramer 4 * 60000, SDS-PAGE Zymomonas mobilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.4.1 NAD+
-
 Zymomonas mobilis