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Literature summary extracted from

  • Elbanna, K.; Lütke-Eversloh, T.; Jendrossek, D.; Luftmann, H.; Steinbüchel, A.
    Studies on the biodegradability of polythioester copolymers and homopolymers by polyhydroxyalkanoate (PHA)-degrading bacteria and PHA depolymerase (2004), Arch. Microbiol., 182, 212-225.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.76 2-mercaptoethanol complete inhibition Schlegelella thermodepolymerans
3.1.1.76 4-hydroxymercuribenzoate
-
 Pseudomonas indica
3.1.1.76 4-hydroxymercuribenzoate complete inhibition Schlegelella thermodepolymerans
3.1.1.76 diisopropylfluorophosphate complete inhibition Schlegelella thermodepolymerans
3.1.1.76 dithiothreitol complete inhibition Schlegelella thermodepolymerans
3.1.1.76 DTNB complete inhibition Schlegelella thermodepolymerans
3.1.1.76 EDTA
-
 Pseudomonas indica
3.1.1.76 EDTA complete inhibition Schlegelella thermodepolymerans
3.1.1.76 ethanol strong inhibition at 10% v/v Schlegelella thermodepolymerans
3.1.1.76 iodoacetate
-
 Pseudomonas indica
3.1.1.76 iodoacetate complete inhibition Schlegelella thermodepolymerans
3.1.1.76 KCl
-
 Schlegelella thermodepolymerans
3.1.1.76 additional information no inhibition by DTT Pseudomonas indica
3.1.1.76 n-Propanol strong inhibition at 10% v/v Schlegelella thermodepolymerans
3.1.1.76 NaCl
-
 Schlegelella thermodepolymerans
3.1.1.76 PMSF 40% inhibition 1.0-10 mM Schlegelella thermodepolymerans
3.1.1.76 Sodium azide
-
 Pseudomonas indica
3.1.1.76 Sodium azide complete inhibition Schlegelella thermodepolymerans
3.1.1.76 Triton X-100 strong inhibition Pseudomonas indica
3.1.1.76 Triton X-100 strong inhibition 0.2-1.0 mM Schlegelella thermodepolymerans
3.1.1.76 Tween 20 66% inhibition 2 mM Schlegelella thermodepolymerans
3.1.1.76 Tween 80 strong inhibition Pseudomonas indica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.76 extracellular
-
 Schlegelella thermodepolymerans
-
-
3.1.1.76 extracellular
-
 Pseudomonas indica
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.76 28000
-
 x * 28000, SDS-PAGE Schlegelella thermodepolymerans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.76 Pseudomonas indica
-
-
-
3.1.1.76 Pseudomonas indica K2 / DSM 16298
-
-
-
3.1.1.76 Schlegelella thermodepolymerans
-
 strain DSM 15344
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.76 to homogeneity by ultrafiltration, affinity-based separation, and gel filtration Pseudomonas indica
3.1.1.76 to homogeneity by ultrafiltration, heat tretament at 70°C for 30 min, and affinity-based separation Schlegelella thermodepolymerans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.76 additional information the bacterium can grow on 3-mercaptopropionate or poly(3-hydroxybutyrate-co-3-mercaptopropionate) as sole carbon source Schlegelella thermodepolymerans
-
3.1.1.76 additional information the bacterium can grow on poly(3-hydroxybutyrate-co-3-mercaptopropionate) as sole carbon source Pseudomonas indica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.76 25
-
 purified enzyme Schlegelella thermodepolymerans
3.1.1.76 26
-
 purified enzyme Pseudomonas indica

Storage Stability

EC Number Storage Stability Organism
3.1.1.76 -20°C to 70°C, purified enzyme, stable for more than 6 months Pseudomonas indica
3.1.1.76 -20°C, purified enzyme, 1 year, 70% remaining activity Schlegelella thermodepolymerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.76 additional information substrate specificity, no activity with homopolymers of 3-mercaptopropionate and 3-mercaptobutyrate Schlegelella thermodepolymerans ?
-
 ?
3.1.1.76 additional information substrate specificity, no activity with homopolymers of 3-mercaptopropionate and 3-mercaptobutyrate, or the co-polymeric poly(3-hydroxybutyrate-co-3-mercaptopropionate) Pseudomonas indica ?
-
 ?
3.1.1.76 additional information substrate specificity, no activity with homopolymers of 3-mercaptopropionate and 3-mercaptobutyrate, or the co-polymeric poly(3-hydroxybutyrate-co-3-mercaptopropionate) Pseudomonas indica K2 / DSM 16298 ?
-
 ?
3.1.1.76 poly(3-hydroxybutyrate-co-3-mercaptopropionate) + H2O co-polymeric substrate, the activity decreases with increasing amount of 3-mercaptopropionate Schlegelella thermodepolymerans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.76 ? x * 28000, SDS-PAGE Schlegelella thermodepolymerans

Synonyms

EC Number Synonyms Comment Organism
3.1.1.76 PHA depolymerase
-
 Schlegelella thermodepolymerans
3.1.1.76 PHA depolymerase
-
 Pseudomonas indica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.76 additional information
-
 optimal bacterial growth at 30°C Pseudomonas indica
3.1.1.76 additional information
-
 optimal bacterial growth at 55°C Schlegelella thermodepolymerans
3.1.1.76 35
-
-
 Pseudomonas indica
3.1.1.76 75 90
-
 Schlegelella thermodepolymerans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.76 15 50
-
 Pseudomonas indica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.76 50
-
 over 90% remaining activity after 24 h Schlegelella thermodepolymerans
3.1.1.76 70
-
 69% remaining activity after 24 h Schlegelella thermodepolymerans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.76 8
-
 assay at Schlegelella thermodepolymerans
3.1.1.76 8
-
 assay at Pseudomonas indica