EC Number | Cloned (Comment) | Organism |
---|---|---|
4.4.1.5 | expression in Saccharomyces cerevisiae | Schizosaccharomyces pombe |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.5 | 0.24 | - |
Phenylglyoxal | pH 7.0, 25°C | Schizosaccharomyces pombe | |
4.4.1.5 | 0.8 | - |
glutathione | pH 7.0, 25°C | Schizosaccharomyces pombe | |
4.4.1.5 | 1.6 | - |
methylglyoxal | pH 7.0, 25°C | Schizosaccharomyces pombe |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.5 | Ca2+ | 20mM, 32.8% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
4.4.1.5 | Co2+ | 1mM, 78.6% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
4.4.1.5 | Fe2+ | 1mM, 8.4% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
4.4.1.5 | Mg2+ | 20mM, 13.7% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
4.4.1.5 | Mn2+ | 1mM, 186% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
4.4.1.5 | Ni2+ | 1mM, 67.9% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe | |
4.4.1.5 | Zn2+ | 0.5mM, 84.7% reactivation of EDTA-treated enzyme | Schizosaccharomyces pombe |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.4.1.5 | 34000 | - |
gel filtration | Schizosaccharomyces pombe |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.4.1.5 | Schizosaccharomyces pombe | - |
PR 109 | - |
4.4.1.5 | Schizosaccharomyces pombe PR 109 | - |
PR 109 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.4.1.5 | - |
Schizosaccharomyces pombe |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.4.1.5 | additional information | - |
Vmax methylglyoxal purified enzyme 2 mmol/min/mg protein, Vmax phenylglyoxal 0.197 mmol/min/mg protein, pH 7.0, 25°C | Schizosaccharomyces pombe |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.5 | glutathione + methylglyoxal | - |
Schizosaccharomyces pombe | (R)-S-lactoylglutathione | - |
? | |
4.4.1.5 | glutathione + methylglyoxal | - |
Schizosaccharomyces pombe PR 109 | (R)-S-lactoylglutathione | - |
? | |
4.4.1.5 | glutathione + phenylglyoxal | - |
Schizosaccharomyces pombe | S-mandeloylglutathione | - |
? | |
4.4.1.5 | glutathione + phenylglyoxal | - |
Schizosaccharomyces pombe PR 109 | S-mandeloylglutathione | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.4.1.5 | monomer | 1 * 34000, SDS-PAGE | Schizosaccharomyces pombe |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.4.1.5 | Glo1 | - |
Schizosaccharomyces pombe |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.4.1.5 | 60 | - |
100% activity after 4 h incubation at 60°C, 50% inactivation after 150 min incubation at 64°C or after 60 min incubation at 65°C | Schizosaccharomyces pombe |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.4.1.5 | 5.5 | 10 | stable at 4°C for at least 24h | Schizosaccharomyces pombe |