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Literature summary extracted from

  • Jog, S.P.; Garchow, B.G.; Mehta, B.D.; Murthy, P.P.N.
    Alkaline phytase from lily pollen: Investigation of biochemical properties (2005), Arch. Biochem. Biophys., 440, 133-140.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.72 EDTA
-
 Lilium longiflorum
3.1.3.72 MgCl2 0.166 M Lilium longiflorum
3.1.3.72 MgSO4 0.5 M Lilium longiflorum
3.1.3.72 vanadate 0.25-3 mM, 78-83% inhibition Lilium longiflorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.72 0.081
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate 37°C, pH 8.0 Lilium longiflorum
3.1.3.72 0.372
-
 p-nitrophenyl phosphate 37°C, pH 7.0 Lilium longiflorum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.72 (NH4)2SO4 0.166 M, enhances activity Lilium longiflorum
3.1.3.72 Ca2+ activates, enzyme retains low level of activity even in Ca2+-free medium Lilium longiflorum
3.1.3.72 K2SO4 0.166 M, enhances activity Lilium longiflorum
3.1.3.72 KCl 0.5 M, activates Lilium longiflorum
3.1.3.72 Na2SO4 0.166 M, enhances activity Lilium longiflorum
3.1.3.72 NaCl 0.5 M, activates Lilium longiflorum
3.1.3.72 NH4Cl 0.5 M, enhances activity Lilium longiflorum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.72 Lilium longiflorum
-
 cv. Nellie White
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.72
-
 Lilium longiflorum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.72 pollen
-
 Lilium longiflorum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.72 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
 Lilium longiflorum myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate
-
 ?
3.1.3.72 p-nitrophenyl phosphate + H2O
-
 Lilium longiflorum p-nitrophenol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.72 alkaline phytase
-
 Lilium longiflorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.72 55
-
-
 Lilium longiflorum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.72 50
-
 10 min, stable Lilium longiflorum
3.1.3.72 55
-
 10 min, 60% loss of activity in assay containing CaCl2, compared to 40% in absence of CaCl2 Lilium longiflorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.72 7
-
 activity with p-nitrophenyl phosphate Lilium longiflorum
3.1.3.72 8
-
 activity with myo-inositol-1,2,3,4,5,6-hexakisphosphate Lilium longiflorum