BRENDA - Enzyme Database

Differential hydrolysis of erythrocyte and mitochondrial membrane phospholipids by two phospholipase A2 isoenzymes (NK-PLA2-I and NK-PLA2-II) from the venom of the Indian monocled cobra Naja kaouthia

Doley, R.; King, G.F.; Mukherjee, A.K.; Arch. Biochem. Biophys. 425, 1-13 (2004)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
additional information
not inhibitory to both isoenzymes: p-methylsulfonylfluoride, tosyl-L-phenylalaninchlormethylketon, N-bromosuccinamide, tosyl-L-lysinechlormethylketon
Naja kaouthia
3.1.1.4
p-bromophenacylbromide
i.e. pBPB, 3.3 mM, 95% inhibition of isoenzyme NK-PLA2-I, 89% inhibition of isoenzyme NK-PLA2-II
Naja kaouthia
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
13100
-
PAGE
Naja kaouthia
3.1.1.4
13346
-
1 * 13100, SDS-PAGE, 1 * 13346, MALDI-MS
Naja kaouthia
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
additional information
Naja kaouthia
both isoenzymes NK-PLA2-I and NK-PLA2-II cause significantly more damage to mitochondrial membranes as compared to erythrocyte membranes
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.4
Naja kaouthia
-
isoenzymes NK-PLA2-I and NK-PLA2-II
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.4
isoenzyme NK-PLA2-II
Naja kaouthia
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
venom
-
Naja kaouthia
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.4
61
-
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C20:4, 25°C; isoenzyme NK-PLA2-I, substrate phosphatidylethanolamine, sn-2 fatty acid C18:1, 25°C
Naja kaouthia
3.1.1.4
69.2
-
isoenzyme NK-PLA2-II, substrate phosphatidylethanolamine, sn-2 fatty acid C18:1, 25°C
Naja kaouthia
3.1.1.4
76.3
-
isoenzyme NK-PLA2-II, substrate phosphatidylethanolamine, sn-2 fatty acid C18:2, 25°C
Naja kaouthia
3.1.1.4
79
-
isoenzyme NK-PLA2-I, substrate phosphatidylethanolamine, sn-2 fatty acid C18:2, 25°C
Naja kaouthia
3.1.1.4
89.3
-
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C20:4, 25°C
Naja kaouthia
3.1.1.4
96.3
-
isoenzyme NK-PLA2-II, substrate phosphatidylserine, sn-2 fatty acid C18:1, 25°C
Naja kaouthia
3.1.1.4
101.2
-
isoenzyme NK-PLA2-I, substrate phosphatidylserine, sn-2 fatty acid C18:1, 25°C
Naja kaouthia
3.1.1.4
133
-
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C16:0, 25°C
Naja kaouthia
3.1.1.4
153.9
-
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C18:0, 25°C
Naja kaouthia
3.1.1.4
178.4
-
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C18:1, 25°C
Naja kaouthia
3.1.1.4
202.3
-
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C18:2, 25°C
Naja kaouthia
3.1.1.4
218.4
-
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C18:2, 25°C
Naja kaouthia
3.1.1.4
220.5
-
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C16:0, 25°C
Naja kaouthia
3.1.1.4
229.9
-
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C18:1, 25°C
Naja kaouthia
3.1.1.4
243.1
-
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C18:0, 25°C
Naja kaouthia
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
additional information
both isoenzymes NK-PLA2-I and NK-PLA2-II cause significantly more damage to mitochondrial membranes as compared to erythrocyte membranes
663759
Naja kaouthia
?
-
-
-
-
3.1.1.4
additional information
isoenzyme NK-PLA2-I does not discriminate between saturated and unsaturated fatty acids whereas isoenzyme NK-PLA2-II shows a preference for unsaturated fatty acids
663759
Naja kaouthia
?
-
-
-
-
3.1.1.4
phosphatidylcholine + H2O
preferred substrate for both isoenzymes, NK-PLA2-I and NK-PLA2-II
663759
Naja kaouthia
1-acylglycerophosphocholine + fatty acid
-
-
-
?
3.1.1.4
phosphatidylethanolamine + H2O
-
663759
Naja kaouthia
1-acylglycerophosphorylethanolamine + fatty acid
-
-
-
?
3.1.1.4
phosphatidylserine + H2O
-
663759
Naja kaouthia
1-acylglycerophosphoserine + fatty acid
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.4
monomer
1 * 13100, SDS-PAGE, 1 * 13346, MALDI-MS
Naja kaouthia
3.1.1.4
More
N-terminal amino acid sequence is identical for both isoenzymes NK-PLA2-I and NK-PLA2-II
Naja kaouthia
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.4
100
-
45 min, isoenzyme NK-PLA2-I, 50% residual activity, isoenzyme NK-PLA2-II, 43% residual activity
Naja kaouthia
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
additional information
not inhibitory to both isoenzymes: p-methylsulfonylfluoride, tosyl-L-phenylalaninchlormethylketon, N-bromosuccinamide, tosyl-L-lysinechlormethylketon
Naja kaouthia
3.1.1.4
p-bromophenacylbromide
i.e. pBPB, 3.3 mM, 95% inhibition of isoenzyme NK-PLA2-I, 89% inhibition of isoenzyme NK-PLA2-II
Naja kaouthia
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
13100
-
PAGE
Naja kaouthia
3.1.1.4
13346
-
1 * 13100, SDS-PAGE, 1 * 13346, MALDI-MS
Naja kaouthia
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
additional information
Naja kaouthia
both isoenzymes NK-PLA2-I and NK-PLA2-II cause significantly more damage to mitochondrial membranes as compared to erythrocyte membranes
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.4
isoenzyme NK-PLA2-II
Naja kaouthia
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
venom
-
Naja kaouthia
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.4
61
-
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C20:4, 25°C; isoenzyme NK-PLA2-I, substrate phosphatidylethanolamine, sn-2 fatty acid C18:1, 25°C
Naja kaouthia
3.1.1.4
69.2
-
isoenzyme NK-PLA2-II, substrate phosphatidylethanolamine, sn-2 fatty acid C18:1, 25°C
Naja kaouthia
3.1.1.4
76.3
-
isoenzyme NK-PLA2-II, substrate phosphatidylethanolamine, sn-2 fatty acid C18:2, 25°C
Naja kaouthia
3.1.1.4
79
-
isoenzyme NK-PLA2-I, substrate phosphatidylethanolamine, sn-2 fatty acid C18:2, 25°C
Naja kaouthia
3.1.1.4
89.3
-
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C20:4, 25°C
Naja kaouthia
3.1.1.4
96.3
-
isoenzyme NK-PLA2-II, substrate phosphatidylserine, sn-2 fatty acid C18:1, 25°C
Naja kaouthia
3.1.1.4
101.2
-
isoenzyme NK-PLA2-I, substrate phosphatidylserine, sn-2 fatty acid C18:1, 25°C
Naja kaouthia
3.1.1.4
133
-
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C16:0, 25°C
Naja kaouthia
3.1.1.4
153.9
-
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C18:0, 25°C
Naja kaouthia
3.1.1.4
178.4
-
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C18:1, 25°C
Naja kaouthia
3.1.1.4
202.3
-
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C18:2, 25°C
Naja kaouthia
3.1.1.4
218.4
-
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C18:2, 25°C
Naja kaouthia
3.1.1.4
220.5
-
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C16:0, 25°C
Naja kaouthia
3.1.1.4
229.9
-
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C18:1, 25°C
Naja kaouthia
3.1.1.4
243.1
-
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C18:0, 25°C
Naja kaouthia
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
additional information
both isoenzymes NK-PLA2-I and NK-PLA2-II cause significantly more damage to mitochondrial membranes as compared to erythrocyte membranes
663759
Naja kaouthia
?
-
-
-
-
3.1.1.4
additional information
isoenzyme NK-PLA2-I does not discriminate between saturated and unsaturated fatty acids whereas isoenzyme NK-PLA2-II shows a preference for unsaturated fatty acids
663759
Naja kaouthia
?
-
-
-
-
3.1.1.4
phosphatidylcholine + H2O
preferred substrate for both isoenzymes, NK-PLA2-I and NK-PLA2-II
663759
Naja kaouthia
1-acylglycerophosphocholine + fatty acid
-
-
-
?
3.1.1.4
phosphatidylethanolamine + H2O
-
663759
Naja kaouthia
1-acylglycerophosphorylethanolamine + fatty acid
-
-
-
?
3.1.1.4
phosphatidylserine + H2O
-
663759
Naja kaouthia
1-acylglycerophosphoserine + fatty acid
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.4
monomer
1 * 13100, SDS-PAGE, 1 * 13346, MALDI-MS
Naja kaouthia
3.1.1.4
More
N-terminal amino acid sequence is identical for both isoenzymes NK-PLA2-I and NK-PLA2-II
Naja kaouthia
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.4
100
-
45 min, isoenzyme NK-PLA2-I, 50% residual activity, isoenzyme NK-PLA2-II, 43% residual activity
Naja kaouthia