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Literature summary extracted from

  • Petrillo, K.L.; Wu, S.; Hann, E.C.; Cooling, F.B.; Ben-Bassat, A.; Gavagan, J.E.; DiCosimo, R.; Payne, M.S.
    Over-expression in Escherichia coli of a thermally stable and regio-selective nitrile hydratase from Comamonas testosteroni 5-MGAM-4D (2005), Appl. Microbiol. Biotechnol., 67, 664-670.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.84
-
 Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.84 23000
-
 x * 23000, alpha-subunit, + x * 24000, beta-subunit, calculated Comamonas testosteroni
4.2.1.84 24000
-
 x * 23000, alpha-subunit, + x * 24000, beta-subunit, calculated Comamonas testosteroni

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Comamonas testosteroni Q5XPL4 beta-subunit; expression in Escherichia coli
-
4.2.1.84 Comamonas testosteroni 5-MGAM-4D Q5XPL4 beta-subunit; expression in Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 3-hydroxybutryronitrile + H2O
-
 Comamonas testosteroni 3-hydroxybutyramide yield 99% ?
4.2.1.84 3-hydroxybutryronitrile + H2O
-
 Comamonas testosteroni 5-MGAM-4D 3-hydroxybutyramide yield 99% ?
4.2.1.84 3-hydroxypropionitrile + H2O
-
 Comamonas testosteroni 3-hydroxypropionamide yield 100% ?
4.2.1.84 3-hydroxypropionitrile + H2O
-
 Comamonas testosteroni 5-MGAM-4D 3-hydroxypropionamide yield 100% ?
4.2.1.84 3-hydroxyvaleronitrile + H2O
-
 Comamonas testosteroni 3-hydroxyvaleramide yield 99% ?
4.2.1.84 3-hydroxyvaleronitrile + H2O
-
 Comamonas testosteroni 5-MGAM-4D 3-hydroxyvaleramide yield 99% ?
4.2.1.84 acrylonitrile + H2O
-
 Comamonas testosteroni acrylamide yield 100% ?
4.2.1.84 acrylonitrile + H2O
-
 Comamonas testosteroni 5-MGAM-4D acrylamide yield 100% ?
4.2.1.84 adiponitrile + 2 H2O
-
 Comamonas testosteroni adipamide yield 100% ?
4.2.1.84 adiponitrile + 2 H2O
-
 Comamonas testosteroni 5-MGAM-4D adipamide yield 100% ?
4.2.1.84 butyronitrile + H2O
-
 Comamonas testosteroni butyramide yield 100% ?
4.2.1.84 glycolonitrile + H2O
-
 Comamonas testosteroni glycolamide yield 63% ?
4.2.1.84 methacrylonitrile + H2O
-
 Comamonas testosteroni methacrylamide yield 100% ?

Subunits

EC Number Subunits Comment Organism
4.2.1.84 ? x * 23000, alpha-subunit, + x * 24000, beta-subunit, calculated Comamonas testosteroni