Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bhatia, Y.; Mishra, S.; Bisaria, V.S.
    Purification and characterization of recombinant Escherichia coli-expressed Pichia etchellsii beta-glucosidase II with high hydrolytic activity on sophorose (2005), Appl. Microbiol. Biotechnol., 66, 527-535.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.21 biotechnology the enzyme might be useful as biocatalyst in the synthesis of glyco-conjugates, overview Schwanniomyces etchellsii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression in Escherichia coli strain JM109 Schwanniomyces etchellsii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 diethyldicarbonate binds to the active site, 4-nitrophenyl beta-D-glucoside partially protects against inactivation Schwanniomyces etchellsii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 83000
-
 2 * 83000, recombinant enzyme Schwanniomyces etchellsii
3.2.1.21 176000
-
 recombinant enzyme Schwanniomyces etchellsii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Schwanniomyces etchellsii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 recombinant enzyme from Escherichia coli strain JM109 Schwanniomyces etchellsii

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.21 celloheptaose + 6 H2O = 7 beta-D-glucose the enzyme contains a His residue which is important for catalytic activity Schwanniomyces etchellsii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 0.77
-
 purified recombinant enzyme, substrate sophorose Schwanniomyces etchellsii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucoside + H2O
-
 Schwanniomyces etchellsii 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 amygdalin + H2O
-
 Schwanniomyces etchellsii ?
-
 ?
3.2.1.21 additional information the enzyme shows broad substrate specificity hydrolyzing beta-linked gluco-disaccharides and oligosaccharides, and transglycosylation activity forming beta-linked di- and oligosaccharides Schwanniomyces etchellsii ?
-
 ?
3.2.1.21 salicin + H2O
-
 Schwanniomyces etchellsii ?
-
 ?
3.2.1.21 sophorose + H2O
-
 Schwanniomyces etchellsii 2 D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 dimer 2 * 83000, recombinant enzyme Schwanniomyces etchellsii

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 beta-glucosidase II
-
 Schwanniomyces etchellsii
3.2.1.21 More the enzyme belongs to the glycosyl hydrolase family 3 Schwanniomyces etchellsii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 additional information
-
 the enzyme is thermotolerant Schwanniomyces etchellsii