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Literature summary extracted from
- Identification of Pyrococcus furiosus amylopullulanase catalytic residues (2005), Appl. Microbiol. Biotechnol., 66, 408-413.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.41 | D394N | kcat/KM is 24.2fold lower than wild-type value, specific activity on starch decreases 11.7times | Pyrococcus furiosus |
| 3.2.1.41 | E291Q | kcat/KM is 123fold lower than wild-type value, specific activity on starch decreases 91times | Pyrococcus furiosus |
| 3.2.1.41 | E396Q | activity of the mutant enzyme on pullulan is to low to allow reliable determination of catalytic efficiency, specific activity on starch decreases 37.2times | Pyrococcus furiosus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.41 | Pyrococcus furiosus | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.41 | pullulan + H2O | - |
Pyrococcus furiosus | maltotriose + ? | - |
? |  |
| 3.2.1.41 | soluble starch + H2O | - |
Pyrococcus furiosus | maltotriose + maltose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.41 | PfAPU | - |
Pyrococcus furiosus |
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Release 2023.1 (January 2023)
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