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Literature summary extracted from

  • Aleksandrzak-Piekarczyk, T.; Kok, J.; Renault, P.; Bardowski, J.
    Alternative lactose catabolic pathway in Lactococcus lactis IL1403 (2005), Appl. Environ. Microbiol., 71, 6060-6069.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.85 subcloning in Escherichia coli, expression of wild-type and mutant enzymes in Lactococcus lactis strain IBB50 Lactococcus lactis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.85 additional information construction of a disruption mutant of gene bglS, encoding phospho-beta-glucosidase, in phospho-beta-galactosidase-deficient strain IL1403, genetic organization and chromosomal localizations in mutant strain IL1403 showig the beta-galactosidase-negative phenotype Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.85 lactose + H2O Lactococcus lactis existence of an alternative lactose catabolic pathway in the enzyme-deficient mutant strain IL1403 by phospho-beta-galactosidase activity exhibited by BglS, a phospho-beta-glucosidase, the lactose hydrolysis is catalyzed by both phospho-beta-galactosidase and phospho-beta-glucosidase, the latter being the main enzyme in the pathway D-glucose + D-galactose
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 ?
3.2.1.85 additional information Lactococcus lactis physiologic regulation of the pathway, overview ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.85 Lactococcus lactis
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 subsp. lactis, wild-type strains and enzyme-deficient mutant strain IL1403, overview
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.85 additional information growth condition requirements of mutant strains Lactococcus lactis
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.85 2-nitrophenyl beta-D-galactopyranoside 6-phosphate + H2O
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 Lactococcus lactis 2-nitrophenyl beta-D-galactopyranose + phosphate
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 ?
3.2.1.85 lactose + H2O
-
 Lactococcus lactis D-glucose + D-galactose
-
 ?
3.2.1.85 lactose + H2O existence of an alternative lactose catabolic pathway in the enzyme-deficient mutant strain IL1403 by phospho-beta-galactosidase activity exhibited by BglS, a phospho-beta-glucosidase, the lactose hydrolysis is catalyzed by both phospho-beta-galactosidase and phospho-beta-glucosidase, the latter being the main enzyme in the pathway Lactococcus lactis D-glucose + D-galactose
-
 ?
3.2.1.85 additional information physiologic regulation of the pathway, overview Lactococcus lactis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.85 P-beta-galactosidase
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 Lactococcus lactis
3.2.1.85 phospho-beta-galactosidase
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 Lactococcus lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.85 30
-
 assay at Lactococcus lactis