Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yang, S.J.; Lee, H.S.; Park, C.S.; Kim, Y.R.; Moon, T.W.; Park, K.H.
    Enzymatic analysis of an amylolytic enzyme from the hyperthermophilic archaeon Pyrococcus furiosus reveals its novel catalytic properties as both an alpha-amylase and a cyclodextrin-hydrolyzing enzyme (2004), Appl. Environ. Microbiol., 70, 5988-5995.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 PTFA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) and MC1061 Pyrococcus furiosus
3.2.1.54 expression in Escherichia coli Pyrococcus furiosus
3.2.1.54 PTFA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) and MC1061 Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 additional information
-
additional information kinetics Pyrococcus furiosus
3.2.1.1 0.52
-
starch pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.1 2.16
-
beta-cyclodextrin pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.1 2.61
-
alpha-cyclodextrin pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.1 5.12
-
gamma-cyclodextrin pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.1 11.1
-
acarbose pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.1 62.9
-
maltotriose pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.54 additional information
-
additional information kinetics Pyrococcus furiosus
3.2.1.54 0.52
-
starch pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.54 2.16
-
beta-cyclodextrin pH 4.5, 90°C Pyrococcus furiosus
3.2.1.54 2.16
-
beta-cyclodextrin pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.54 2.61
-
alpha-cyclodextrin pH 4.5, 90°C Pyrococcus furiosus
3.2.1.54 2.61
-
alpha-cyclodextrin pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.54 5.12
-
gamma-cyclodextrin pH 4.5, 90°C Pyrococcus furiosus
3.2.1.54 5.12
-
gamma-cyclodextrin pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.54 11.1
-
acarbose pH 4.5, 90°C Pyrococcus furiosus
3.2.1.54 11.1
-
acarbose pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.54 62.9
-
maltotriose pH 4.5, 90°C Pyrococcus furiosus
3.2.1.54 62.9
-
maltotriose pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 76084
-
x * 76084, amino acid sequence calculation Pyrococcus furiosus
3.2.1.54 76084
-
x * 76084, amino acid sequence calculation Pyrococcus furiosus
3.2.1.54 76084
-
x * 76084, calculated from sequence Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Pyrococcus furiosus
-
bifunctional enzyme
-
3.2.1.54 Pyrococcus furiosus
-
-
-
3.2.1.54 Pyrococcus furiosus
-
bifunctional enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 recombinant PFTA 32.6fold from Escherichia coli Pyrococcus furiosus
3.2.1.54
-
Pyrococcus furiosus
3.2.1.54 recombinant PFTA 32.6fold from Escherichia coli Pyrococcus furiosus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.1 (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-m-alpha-D-glucopyranose + (alpha-D-glucopyranosyl-(1-4))m-alpha-D-glucopyranose PFTA is a bifunctional enzyme showing alpha-amylase as well as cyclodextrin-hydrolyzing activity Pyrococcus furiosus
3.2.1.54 cyclomaltodextrin + H2O = linear maltodextrin PFTA is a bifunctional enzyme showing alpha-amylase as well as cyclodextrin-hydrolyzing activity Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 additional information
-
-
Pyrococcus furiosus
3.2.1.1 176.4
-
purified recombinant enzyme, substrate beta-cyclodextrin Pyrococcus furiosus
3.2.1.54 additional information
-
-
Pyrococcus furiosus
3.2.1.54 176.4
-
-
Pyrococcus furiosus
3.2.1.54 176.4
-
purified recombinant enzyme, substrate beta-cyclodextrin Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 4-nitrophenyl-alpha-D-maltopentaoside + H2O
-
Pyrococcus furiosus 4-nitrophenol + alpha-D-maltopentaoside
-
?
3.2.1.1 acarbose + H2O the substrate is a potent inhibitor of alpha-amylases, cyclomaltodextrinase activity Pyrococcus furiosus ?
-
?
3.2.1.1 alpha-cyclodextrin + H2O cyclomaltodextrinase activity Pyrococcus furiosus ?
-
?
3.2.1.1 beta-cyclodextrin + H2O cyclomaltodextrinase activity Pyrococcus furiosus panose + maltoheptaose
-
?
3.2.1.1 gamma-cyclodextrin + H2O cyclomaltodextrinase activity Pyrococcus furiosus ?
-
?
3.2.1.1 maltooligosaccharides + H2O alpha-amylase activity Pyrococcus furiosus maltotriose + maltotetraose main products ?
3.2.1.1 maltotriose + H2O very low activity Pyrococcus furiosus ?
-
?
3.2.1.1 additional information substrate specificity, hydrolytic pattern, PFTA is a bifunctional enzyme showing alpha-amylase as well as cyclodextrin-hydrolyzing activity, but no transglycosylation activity, overview Pyrococcus furiosus ?
-
?
3.2.1.1 pullulan + H2O cyclomaltodextrinase activity Pyrococcus furiosus panose + maltoheptaose
-
?
3.2.1.1 starch + H2O alpha-amylase activity, preferred substrate Pyrococcus furiosus maltotriose + maltotetraose main products ?
3.2.1.54 4-nitrophenyl-alpha-D-maltopentaoside + H2O
-
Pyrococcus furiosus 4-nitrophenol + alpha-D-maltopentaoside
-
?
3.2.1.54 acarbose + H2O the substrate is a potent inhibitor of alpha-amylases, cyclomaltodextrinase activity Pyrococcus furiosus ?
-
?
3.2.1.54 acarbose + H2O
-
Pyrococcus furiosus acarviosine-glucose + alpha-D-glucose
-
?
3.2.1.54 alpha-cyclodextrin + H2O
-
Pyrococcus furiosus ?
-
?
3.2.1.54 alpha-cyclodextrin + H2O cyclomaltodextrinase activity Pyrococcus furiosus ?
-
?
3.2.1.54 beta-cyclodextrin + H2O
-
Pyrococcus furiosus maltooligosaccharides G1 to G7, maltoheptaose as major product ?
3.2.1.54 beta-cyclodextrin + H2O cyclomaltodextrinase activity Pyrococcus furiosus panose + maltoheptaose
-
?
3.2.1.54 gamma-cyclodextrin + H2O
-
Pyrococcus furiosus ?
-
?
3.2.1.54 gamma-cyclodextrin + H2O cyclomaltodextrinase activity Pyrococcus furiosus ?
-
?
3.2.1.54 maltooligosaccharides + H2O alpha-amylase activity Pyrococcus furiosus maltotriose + maltotetraose main products ?
3.2.1.54 maltotriose + H2O
-
Pyrococcus furiosus ?
-
?
3.2.1.54 maltotriose + H2O very low activity Pyrococcus furiosus ?
-
?
3.2.1.54 additional information substrate specificity, hydrolytic pattern, PFTA is a bifunctional enzyme showing alpha-amylase as well as cyclodextrin-hydrolyzing activity, but no transglycosylation activity, overview Pyrococcus furiosus ?
-
?
3.2.1.54 pullulan + H2O
-
Pyrococcus furiosus panose + beta-cyclodextrin
-
?
3.2.1.54 pullulan + H2O cyclomaltodextrinase activity Pyrococcus furiosus panose + maltoheptaose
-
?
3.2.1.54 soluble starch + H2O
-
Pyrococcus furiosus ?
-
?
3.2.1.54 starch + H2O alpha-amylase activity, preferred substrate Pyrococcus furiosus maltotriose + maltotetraose main products ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ? x * 76084, amino acid sequence calculation Pyrococcus furiosus
3.2.1.1 More primary structure scheme Pyrococcus furiosus
3.2.1.54 ? x * 76084, amino acid sequence calculation Pyrococcus furiosus
3.2.1.54 ? x * 76084, calculated from sequence Pyrococcus furiosus
3.2.1.54 More primary structure scheme Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 More cf. EC 3.2.1.54, cyclomaltodextrinase, the enzyme belongs to glycoside hydrolase family 13 Pyrococcus furiosus
3.2.1.1 PFTA
-
Pyrococcus furiosus
3.2.1.54 More cf. EC 3.2.1.1, alpha-amylase, the enzyme belongs to glycoside hydrolase family 13 Pyrococcus furiosus
3.2.1.54 PFTA
-
Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 90
-
recombinant enzyme Pyrococcus furiosus
3.2.1.54 90
-
-
Pyrococcus furiosus
3.2.1.54 90
-
recombinant enzyme Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.54 70 100 70°C: about 60% of maximal activity, 95°C: about 70% of maximal activity, 100°C: about 20% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 additional information
-
highly thermostable recombinant enzyme Pyrococcus furiosus
3.2.1.1 85
-
purified recombinant enzyme, sodium acetate buffer, pH 5.0, completely stable for 120 min Pyrococcus furiosus
3.2.1.1 90
-
purified recombinant enzyme, sodium acetate buffer, pH 5.0, loss of 15-20% activity within 120 min Pyrococcus furiosus
3.2.1.1 95
-
purified recombinant enzyme, sodium acetate buffer, pH 5.0, loss of 25% activity within 120 min Pyrococcus furiosus
3.2.1.1 100
-
purified recombinant enzyme, sodium acetate buffer, pH 5.0, loss of 65% activity within 60 min Pyrococcus furiosus
3.2.1.54 additional information
-
highly thermostable recombinant enzyme Pyrococcus furiosus
3.2.1.54 85
-
120 min, stable Pyrococcus furiosus
3.2.1.54 85
-
purified recombinant enzyme, sodium acetate buffer, pH 5.0, completely stable for 120 min Pyrococcus furiosus
3.2.1.54 90
-
half-life: 398 min Pyrococcus furiosus
3.2.1.54 90
-
purified recombinant enzyme, sodium acetate buffer, pH 5.0, loss of 15-20% activity within 120 min Pyrococcus furiosus
3.2.1.54 95
-
half-life: 114 min Pyrococcus furiosus
3.2.1.54 95
-
purified recombinant enzyme, sodium acetate buffer, pH 5.0, loss of 25% activity within 120 min Pyrococcus furiosus
3.2.1.54 100
-
1 h, more than 27% of initial activity remains Pyrococcus furiosus
3.2.1.54 100
-
purified recombinant enzyme, sodium acetate buffer, pH 5.0, loss of 65% activity within 60 min Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.1 67
-
starch pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.1 173
-
gamma-cyclodextrin pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.1 196
-
beta-cyclodextrin pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.1 228
-
acarbose pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.1 241
-
alpha-cyclodextrin pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.1 268
-
maltotriose pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.54 67
-
starch pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.54 173
-
gamma-cyclodextrin pH 4.5, 90°C Pyrococcus furiosus
3.2.1.54 173
-
gamma-cyclodextrin pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.54 196
-
beta-cyclodextrin pH 4.5, 90°C Pyrococcus furiosus
3.2.1.54 196
-
beta-cyclodextrin pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.54 228
-
acarbose pH 4.5, 90°C Pyrococcus furiosus
3.2.1.54 228
-
acarbose pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.54 241
-
alpha-cyclodextrin pH 4.5, 90°C Pyrococcus furiosus
3.2.1.54 241
-
alpha-cyclodextrin pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus
3.2.1.54 268
-
maltotriose pH 4.5, 90°C Pyrococcus furiosus
3.2.1.54 268
-
maltotriose pH 4.5, 90°C, recombinant enzyme Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 4.5
-
assay at Pyrococcus furiosus
3.2.1.54 4.5
-
-
Pyrococcus furiosus
3.2.1.54 4.5
-
assay at Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.54 4 6 about 50% of maximal activity at pH 4.0 and 6.0 Pyrococcus furiosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.54 2.5
-
37°C, 24 h, complete inactivation Pyrococcus furiosus
3.2.1.54 3
-
37°C, 24 h, 20% loss of activity Pyrococcus furiosus
3.2.1.54 4 11 37°C, 24 h, stable Pyrococcus furiosus