EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.1 | PTFA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) and MC1061 | Pyrococcus furiosus |
3.2.1.54 | expression in Escherichia coli | Pyrococcus furiosus |
3.2.1.54 | PTFA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) and MC1061 | Pyrococcus furiosus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | additional information | - |
additional information | kinetics | Pyrococcus furiosus | |
3.2.1.1 | 0.52 | - |
starch | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.1 | 2.16 | - |
beta-cyclodextrin | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.1 | 2.61 | - |
alpha-cyclodextrin | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.1 | 5.12 | - |
gamma-cyclodextrin | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.1 | 11.1 | - |
acarbose | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.1 | 62.9 | - |
maltotriose | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.54 | additional information | - |
additional information | kinetics | Pyrococcus furiosus | |
3.2.1.54 | 0.52 | - |
starch | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.54 | 2.16 | - |
beta-cyclodextrin | pH 4.5, 90°C | Pyrococcus furiosus | |
3.2.1.54 | 2.16 | - |
beta-cyclodextrin | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.54 | 2.61 | - |
alpha-cyclodextrin | pH 4.5, 90°C | Pyrococcus furiosus | |
3.2.1.54 | 2.61 | - |
alpha-cyclodextrin | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.54 | 5.12 | - |
gamma-cyclodextrin | pH 4.5, 90°C | Pyrococcus furiosus | |
3.2.1.54 | 5.12 | - |
gamma-cyclodextrin | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.54 | 11.1 | - |
acarbose | pH 4.5, 90°C | Pyrococcus furiosus | |
3.2.1.54 | 11.1 | - |
acarbose | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.54 | 62.9 | - |
maltotriose | pH 4.5, 90°C | Pyrococcus furiosus | |
3.2.1.54 | 62.9 | - |
maltotriose | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 76084 | - |
x * 76084, amino acid sequence calculation | Pyrococcus furiosus |
3.2.1.54 | 76084 | - |
x * 76084, amino acid sequence calculation | Pyrococcus furiosus |
3.2.1.54 | 76084 | - |
x * 76084, calculated from sequence | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.1 | Pyrococcus furiosus | - |
bifunctional enzyme | - |
3.2.1.54 | Pyrococcus furiosus | - |
- |
- |
3.2.1.54 | Pyrococcus furiosus | - |
bifunctional enzyme | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.1 | recombinant PFTA 32.6fold from Escherichia coli | Pyrococcus furiosus |
3.2.1.54 | - |
Pyrococcus furiosus |
3.2.1.54 | recombinant PFTA 32.6fold from Escherichia coli | Pyrococcus furiosus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.2.1.1 | (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-m-alpha-D-glucopyranose + (alpha-D-glucopyranosyl-(1-4))m-alpha-D-glucopyranose | PFTA is a bifunctional enzyme showing alpha-amylase as well as cyclodextrin-hydrolyzing activity | Pyrococcus furiosus | |
3.2.1.54 | cyclomaltodextrin + H2O = linear maltodextrin | PFTA is a bifunctional enzyme showing alpha-amylase as well as cyclodextrin-hydrolyzing activity | Pyrococcus furiosus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | additional information | - |
- |
Pyrococcus furiosus |
3.2.1.1 | 176.4 | - |
purified recombinant enzyme, substrate beta-cyclodextrin | Pyrococcus furiosus |
3.2.1.54 | additional information | - |
- |
Pyrococcus furiosus |
3.2.1.54 | 176.4 | - |
- |
Pyrococcus furiosus |
3.2.1.54 | 176.4 | - |
purified recombinant enzyme, substrate beta-cyclodextrin | Pyrococcus furiosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.1 | 4-nitrophenyl-alpha-D-maltopentaoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + alpha-D-maltopentaoside | - |
? | |
3.2.1.1 | acarbose + H2O | the substrate is a potent inhibitor of alpha-amylases, cyclomaltodextrinase activity | Pyrococcus furiosus | ? | - |
? | |
3.2.1.1 | alpha-cyclodextrin + H2O | cyclomaltodextrinase activity | Pyrococcus furiosus | ? | - |
? | |
3.2.1.1 | beta-cyclodextrin + H2O | cyclomaltodextrinase activity | Pyrococcus furiosus | panose + maltoheptaose | - |
? | |
3.2.1.1 | gamma-cyclodextrin + H2O | cyclomaltodextrinase activity | Pyrococcus furiosus | ? | - |
? | |
3.2.1.1 | maltooligosaccharides + H2O | alpha-amylase activity | Pyrococcus furiosus | maltotriose + maltotetraose | main products | ? | |
3.2.1.1 | maltotriose + H2O | very low activity | Pyrococcus furiosus | ? | - |
? | |
3.2.1.1 | additional information | substrate specificity, hydrolytic pattern, PFTA is a bifunctional enzyme showing alpha-amylase as well as cyclodextrin-hydrolyzing activity, but no transglycosylation activity, overview | Pyrococcus furiosus | ? | - |
? | |
3.2.1.1 | pullulan + H2O | cyclomaltodextrinase activity | Pyrococcus furiosus | panose + maltoheptaose | - |
? | |
3.2.1.1 | starch + H2O | alpha-amylase activity, preferred substrate | Pyrococcus furiosus | maltotriose + maltotetraose | main products | ? | |
3.2.1.54 | 4-nitrophenyl-alpha-D-maltopentaoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + alpha-D-maltopentaoside | - |
? | |
3.2.1.54 | acarbose + H2O | the substrate is a potent inhibitor of alpha-amylases, cyclomaltodextrinase activity | Pyrococcus furiosus | ? | - |
? | |
3.2.1.54 | acarbose + H2O | - |
Pyrococcus furiosus | acarviosine-glucose + alpha-D-glucose | - |
? | |
3.2.1.54 | alpha-cyclodextrin + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
3.2.1.54 | alpha-cyclodextrin + H2O | cyclomaltodextrinase activity | Pyrococcus furiosus | ? | - |
? | |
3.2.1.54 | beta-cyclodextrin + H2O | - |
Pyrococcus furiosus | maltooligosaccharides | G1 to G7, maltoheptaose as major product | ? | |
3.2.1.54 | beta-cyclodextrin + H2O | cyclomaltodextrinase activity | Pyrococcus furiosus | panose + maltoheptaose | - |
? | |
3.2.1.54 | gamma-cyclodextrin + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
3.2.1.54 | gamma-cyclodextrin + H2O | cyclomaltodextrinase activity | Pyrococcus furiosus | ? | - |
? | |
3.2.1.54 | maltooligosaccharides + H2O | alpha-amylase activity | Pyrococcus furiosus | maltotriose + maltotetraose | main products | ? | |
3.2.1.54 | maltotriose + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
3.2.1.54 | maltotriose + H2O | very low activity | Pyrococcus furiosus | ? | - |
? | |
3.2.1.54 | additional information | substrate specificity, hydrolytic pattern, PFTA is a bifunctional enzyme showing alpha-amylase as well as cyclodextrin-hydrolyzing activity, but no transglycosylation activity, overview | Pyrococcus furiosus | ? | - |
? | |
3.2.1.54 | pullulan + H2O | - |
Pyrococcus furiosus | panose + beta-cyclodextrin | - |
? | |
3.2.1.54 | pullulan + H2O | cyclomaltodextrinase activity | Pyrococcus furiosus | panose + maltoheptaose | - |
? | |
3.2.1.54 | soluble starch + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
3.2.1.54 | starch + H2O | alpha-amylase activity, preferred substrate | Pyrococcus furiosus | maltotriose + maltotetraose | main products | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.1 | ? | x * 76084, amino acid sequence calculation | Pyrococcus furiosus |
3.2.1.1 | More | primary structure scheme | Pyrococcus furiosus |
3.2.1.54 | ? | x * 76084, amino acid sequence calculation | Pyrococcus furiosus |
3.2.1.54 | ? | x * 76084, calculated from sequence | Pyrococcus furiosus |
3.2.1.54 | More | primary structure scheme | Pyrococcus furiosus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.1 | More | cf. EC 3.2.1.54, cyclomaltodextrinase, the enzyme belongs to glycoside hydrolase family 13 | Pyrococcus furiosus |
3.2.1.1 | PFTA | - |
Pyrococcus furiosus |
3.2.1.54 | More | cf. EC 3.2.1.1, alpha-amylase, the enzyme belongs to glycoside hydrolase family 13 | Pyrococcus furiosus |
3.2.1.54 | PFTA | - |
Pyrococcus furiosus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 90 | - |
recombinant enzyme | Pyrococcus furiosus |
3.2.1.54 | 90 | - |
- |
Pyrococcus furiosus |
3.2.1.54 | 90 | - |
recombinant enzyme | Pyrococcus furiosus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.54 | 70 | 100 | 70°C: about 60% of maximal activity, 95°C: about 70% of maximal activity, 100°C: about 20% of maximal activity | Pyrococcus furiosus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | additional information | - |
highly thermostable recombinant enzyme | Pyrococcus furiosus |
3.2.1.1 | 85 | - |
purified recombinant enzyme, sodium acetate buffer, pH 5.0, completely stable for 120 min | Pyrococcus furiosus |
3.2.1.1 | 90 | - |
purified recombinant enzyme, sodium acetate buffer, pH 5.0, loss of 15-20% activity within 120 min | Pyrococcus furiosus |
3.2.1.1 | 95 | - |
purified recombinant enzyme, sodium acetate buffer, pH 5.0, loss of 25% activity within 120 min | Pyrococcus furiosus |
3.2.1.1 | 100 | - |
purified recombinant enzyme, sodium acetate buffer, pH 5.0, loss of 65% activity within 60 min | Pyrococcus furiosus |
3.2.1.54 | additional information | - |
highly thermostable recombinant enzyme | Pyrococcus furiosus |
3.2.1.54 | 85 | - |
120 min, stable | Pyrococcus furiosus |
3.2.1.54 | 85 | - |
purified recombinant enzyme, sodium acetate buffer, pH 5.0, completely stable for 120 min | Pyrococcus furiosus |
3.2.1.54 | 90 | - |
half-life: 398 min | Pyrococcus furiosus |
3.2.1.54 | 90 | - |
purified recombinant enzyme, sodium acetate buffer, pH 5.0, loss of 15-20% activity within 120 min | Pyrococcus furiosus |
3.2.1.54 | 95 | - |
half-life: 114 min | Pyrococcus furiosus |
3.2.1.54 | 95 | - |
purified recombinant enzyme, sodium acetate buffer, pH 5.0, loss of 25% activity within 120 min | Pyrococcus furiosus |
3.2.1.54 | 100 | - |
1 h, more than 27% of initial activity remains | Pyrococcus furiosus |
3.2.1.54 | 100 | - |
purified recombinant enzyme, sodium acetate buffer, pH 5.0, loss of 65% activity within 60 min | Pyrococcus furiosus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | 67 | - |
starch | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.1 | 173 | - |
gamma-cyclodextrin | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.1 | 196 | - |
beta-cyclodextrin | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.1 | 228 | - |
acarbose | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.1 | 241 | - |
alpha-cyclodextrin | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.1 | 268 | - |
maltotriose | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.54 | 67 | - |
starch | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.54 | 173 | - |
gamma-cyclodextrin | pH 4.5, 90°C | Pyrococcus furiosus | |
3.2.1.54 | 173 | - |
gamma-cyclodextrin | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.54 | 196 | - |
beta-cyclodextrin | pH 4.5, 90°C | Pyrococcus furiosus | |
3.2.1.54 | 196 | - |
beta-cyclodextrin | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.54 | 228 | - |
acarbose | pH 4.5, 90°C | Pyrococcus furiosus | |
3.2.1.54 | 228 | - |
acarbose | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.54 | 241 | - |
alpha-cyclodextrin | pH 4.5, 90°C | Pyrococcus furiosus | |
3.2.1.54 | 241 | - |
alpha-cyclodextrin | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
3.2.1.54 | 268 | - |
maltotriose | pH 4.5, 90°C | Pyrococcus furiosus | |
3.2.1.54 | 268 | - |
maltotriose | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 4.5 | - |
assay at | Pyrococcus furiosus |
3.2.1.54 | 4.5 | - |
- |
Pyrococcus furiosus |
3.2.1.54 | 4.5 | - |
assay at | Pyrococcus furiosus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.54 | 4 | 6 | about 50% of maximal activity at pH 4.0 and 6.0 | Pyrococcus furiosus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.54 | 2.5 | - |
37°C, 24 h, complete inactivation | Pyrococcus furiosus |
3.2.1.54 | 3 | - |
37°C, 24 h, 20% loss of activity | Pyrococcus furiosus |
3.2.1.54 | 4 | 11 | 37°C, 24 h, stable | Pyrococcus furiosus |