EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.132 | synthesis | valuable enzyme for the commercial production of chitosan oligosaccharides and other chitosan hydrolysates | Bacillus sp. (in: Bacteria) |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.132 | - |
Bacillus sp. (in: Bacteria) |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.132 | Hg2+ | 1 mM, 68% inhibition | Bacillus sp. (in: Bacteria) | |
3.2.1.132 | KCl | 0.4 M, 50% inhibition | Bacillus sp. (in: Bacteria) | |
3.2.1.132 | Mn2+ | 1 mM, 69% inhibition | Bacillus sp. (in: Bacteria) | |
3.2.1.132 | NaCl | 0.4 M, 50% inhibition | Bacillus sp. (in: Bacteria) |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.132 | 45000 | - |
x * 45000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.132 | Bacillus sp. (in: Bacteria) | Q9ALZ1 | - |
- |
3.2.1.132 | Bacillus sp. (in: Bacteria) KCTC 0377BP | Q9ALZ1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.132 | - |
Bacillus sp. (in: Bacteria) |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.132 | 1700 | - |
- |
Bacillus sp. (in: Bacteria) |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.132 | chitoheptaose + H2O | 23.8% of the activity with chitosan | Bacillus sp. (in: Bacteria) | ? | - |
? | |
3.2.1.132 | chitoheptaose + H2O | 23.8% of the activity with chitosan | Bacillus sp. (in: Bacteria) KCTC 0377BP | ? | - |
? | |
3.2.1.132 | chitohexaose + H2O | 20.0% of the activity with chitosan | Bacillus sp. (in: Bacteria) | ? | - |
? | |
3.2.1.132 | chitopentaose + H2O | 16.8% of the activity with chitosan | Bacillus sp. (in: Bacteria) | ? | - |
? | |
3.2.1.132 | chitopentaose + H2O | 16.8% of the activity with chitosan | Bacillus sp. (in: Bacteria) KCTC 0377BP | ? | - |
? | |
3.2.1.132 | chitosan + H2O | activity of the enzyme increases with an increase of the degrees of deacetylation of the chitosan substrate | Bacillus sp. (in: Bacteria) | chitosan oligosaccharides | - |
? | |
3.2.1.132 | chitosan + H2O | activity of the enzyme increases with an increase of the degrees of deacetylation of the chitosan substrate | Bacillus sp. (in: Bacteria) KCTC 0377BP | chitosan oligosaccharides | - |
? | |
3.2.1.132 | chitotetraose + H2O | 15.0% of the activity with chitosan | Bacillus sp. (in: Bacteria) | ? | - |
? | |
3.2.1.132 | chitotetraose + H2O | 15.0% of the activity with chitosan | Bacillus sp. (in: Bacteria) KCTC 0377BP | ? | - |
? | |
3.2.1.132 | additional information | no hydrolysis of chitobiose and chitotriose | Bacillus sp. (in: Bacteria) | ? | - |
? | |
3.2.1.132 | additional information | no hydrolysis of chitobiose and chitotriose | Bacillus sp. (in: Bacteria) KCTC 0377BP | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.132 | ? | x * 45000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.132 | 60 | - |
- |
Bacillus sp. (in: Bacteria) |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.132 | 45 | - |
24 h, enzyme retains full activity | Bacillus sp. (in: Bacteria) |
3.2.1.132 | 50 | - |
half-life: 5 h | Bacillus sp. (in: Bacteria) |
3.2.1.132 | 55 | - |
half-life: 10 min | Bacillus sp. (in: Bacteria) |
3.2.1.132 | 60 | - |
half-life: 5 min | Bacillus sp. (in: Bacteria) |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.132 | 2 | - |
1 h, complete inactivation | Bacillus sp. (in: Bacteria) |
3.2.1.132 | 4 | 8 | 1 h, stable | Bacillus sp. (in: Bacteria) |