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Literature summary extracted from

  • Shabtai, Y.; Daya-Mishne, N.
    Production, purification, and properties of a lipase from a bacterium (Pseudomonas aeruginosa YS-7) capable of growing in water-restricted environments (1992), Appl. Environ. Microbiol., 58, 174-180.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 Dimethyl formamide concentrated solution increases the enzyme activity 2fold, maximal activity at a water content of 0.5-1% w/w Pseudomonas aeruginosa
3.1.1.3 dimethyl sulfoxide concentrated solution increases the enzyme activity 5fold, maximal activity at a water content of 0.5-1% w/w Pseudomonas aeruginosa
3.1.1.3 additional information alcohols and glycols up to 20% activate the enzyme, high concentrations inhibit, the extracellular enzyme is induced by triolein, soybean oil, Tween 20, Tween 80, and especially by free fatty acids, e.g. oleic acid or lauric acid more than 25fold Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 acetonitrile
-
 Pseudomonas aeruginosa
3.1.1.3 additional information alcohols and glycols up to 20% activates the enzyme, high concentrations inhibit Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular secretion of the enzyme to culture medium Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 40000
-
 x * 40000, about, SDS-PAGE Pseudomonas aeruginosa

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 dimethyl formamide the purified enzyme is stable for over 30 days at 20-55°C in presence of water-miscible organic solvents such as alcohols, glycols, pyridine, acetonitrile, dimethyl formamide, or dimethyl sulfoxide Pseudomonas aeruginosa
3.1.1.3 dimethyl sulfoxide the purified enzyme is stable for over 30 days at 20-55°C in presence of water-miscible organic solvents such as alcohols, glycols, pyridine, acetonitrile, dimethyl formamide, or dimethyl sulfoxide Pseudomonas aeruginosa
3.1.1.3 additional information the purified enzyme is stable for over 30 days at 20-55°C in presence of water-miscible organic solvents such as alcohols, glycols, pyridine, acetonitrile, dimethyl formamide, or dimethylsulfoxide Pseudomonas aeruginosa
3.1.1.3 Pyridine the purified enzyme is stable for over 30 days at 20-55°C in presence of water-miscible organic solvents such as alcohols, glycols, pyridine, acetonitrile, dimethyl formamide, or dimethylsulfoxide Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Pseudomonas aeruginosa
-
-
-
3.1.1.3 Pseudomonas aeruginosa YS-7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 native extracellular enzyme from culture supernatant by acetone precipitation and hydrophobic interaction chromatography Pseudomonas aeruginosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 additional information culture conditions evaluation, overview Pseudomonas aeruginosa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 22.22
-
 purified enzyme Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl laurate + H2O high activity in a water-restricted environment containing a water content of 0.5-1% w/w Pseudomonas aeruginosa 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O high activity in a water-restricted environment containing a water content of 0.5-1% w/w Pseudomonas aeruginosa YS-7 4-nitrophenol + laurate
-
 ?
3.1.1.3 soybean oil + H2O concentrated soybean oil, high activity in a water-restricted environment containing a water content of 0.5-1% w/w Pseudomonas aeruginosa ?
-
 ?
3.1.1.3 soybean oil + H2O concentrated soybean oil, high activity in a water-restricted environment containing a water content of 0.5-1% w/w Pseudomonas aeruginosa YS-7 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 40000, about, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 20 55 purified enzyme, stable for over 30 days in presence of water-miscible organic solvents such as alcohols, glycols, pyridine, acetonitrile, dimethyl formamide, or dimethyl sulfoxide Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 7
-
-
 Pseudomonas aeruginosa