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Literature summary extracted from
- Inhibition, reactivation, and determination of metal ions in membrane metalloproteases of bacterial origin using high-performance liquid chromatography coupled on-line with inductively coupled plasma mass spectrometry (1997), Anal. Biochem., 252, 277-285.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.40 | 1,10-phenanthroline | 30 min, room temperature, pH 7.5, complete and irreversible inhibition in a concentration range below 1 mM | Pseudomonas aeruginosa |  |
| 3.4.24.40 | EDTA | 30 min, room temperature, pH 7.5, complete inhibition in a concentration range below 1 mM | Pseudomonas aeruginosa | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.40 | Zn2+ | one Zn2+ per protein molecule | Pseudomonas aeruginosa | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.40 | Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.40 | metal chelate chromatography with copper and hydrophobic interaction chromatography, dialysis | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.40 | 125I-insulin + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| 3.4.24.40 | azocasein + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
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