Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sugisawa, T.; Hoshino, T.; Fujiwara, A.
    Purification and properties of NADPH-linked L-sorbose reductase from Gluconobacter melanogenus N44-1 (1991), Agric. Biol. Chem., 55, 2043-2049.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.289 additional information not inhibitory: sodium fluoroacetate, sodium fluoride, KCN, monoiodoacetate Gluconobacter oxydans
1.1.1.289 N-ethylmaleimide 0.97 mM, 81% residual activity Gluconobacter oxydans
1.1.1.289 Na2HAsO4 0.97 mM, 90% residual activity Gluconobacter oxydans
1.1.1.289 p-chloromercuribenzoate 0.19 mM, 43% residual activity Gluconobacter oxydans
1.1.1.289 Quinine 0.49 mM, complete loss of activity Gluconobacter oxydans
1.1.1.289 Sodium azide 0.97 mM, 90% residual activity Gluconobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.289 0.111
-
 NADPH pH 7.0, 25°C Gluconobacter oxydans
1.1.1.289 7.3
-
 D-mannitol pH 10.0, 25°C Gluconobacter oxydans
1.1.1.289 160
-
 D-fructose pH 7.0, 25°C Gluconobacter oxydans
1.1.1.289 167
-
 D-sorbitol pH 10.0, 25°C Gluconobacter oxydans
1.1.1.289 328
-
 L-sorbose pH 7.0, 25°C Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.289 cytosol
-
 Gluconobacter oxydans 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.289 58000
-
 gel filtration Gluconobacter oxydans
1.1.1.289 60000
-
 1 * 60000, SDS-PAGE Gluconobacter oxydans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.289 Gluconobacter oxydans
-
-
-
1.1.1.289 Gluconobacter oxydans N44-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.289
-
 Gluconobacter oxydans

Storage Stability

EC Number Storage Stability Organism
1.1.1.289 -20°C, 0.01 M potassium buffer, pH 7.0, stable for at least one month Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.289 5-keto-D-fructose + NADPH 86.8% of the rate with L-sorbose Gluconobacter oxydans ? + NADP+
-
 r
1.1.1.289 5-keto-D-fructose + NADPH 86.8% of the rate with L-sorbose Gluconobacter oxydans N44-1 ? + NADP+
-
 r
1.1.1.289 D-fructose + NADPH
-
 Gluconobacter oxydans D-mannitol + NADP+
-
 ?
1.1.1.289 D-fructose + NADPH
-
 Gluconobacter oxydans N44-1 D-mannitol + NADP+
-
 ?
1.1.1.289 D-mannitol + NADP+ 134% of the rate with L-sorbose Gluconobacter oxydans L-fructose + NADPH + H+
-
 r
1.1.1.289 D-mannitol + NADP+ 134% of the rate with L-sorbose Gluconobacter oxydans N44-1 L-fructose + NADPH + H+
-
 r
1.1.1.289 D-sorbitol + NADP+ 134% of the rate with L-sorbose Gluconobacter oxydans L-sorbose + NADPH + H+
-
 r
1.1.1.289 D-sorbitol + NADP+ 134% of the rate with L-sorbose Gluconobacter oxydans N44-1 L-sorbose + NADPH + H+
-
 r
1.1.1.289 erythritol + NADP+ 65.3% of the rate with L-sorbose Gluconobacter oxydans ? + NADPH
-
 r
1.1.1.289 erythritol + NADP+ 65.3% of the rate with L-sorbose Gluconobacter oxydans N44-1 ? + NADPH
-
 r
1.1.1.289 L-fructose + NADPH 142% of the rate with L-sorbose Gluconobacter oxydans D-mannitol + NADP+
-
 r
1.1.1.289 L-sorbose + NADPH + H+
-
 Gluconobacter oxydans D-sorbitol + NADP+
-
 r
1.1.1.289 ribulose + NADPH 2.4% of the rate with L-sorbose Gluconobacter oxydans ? + NADP+
-
 r
1.1.1.289 tagatose + NADPH 0.8% of the rate with L-sorbose Gluconobacter oxydans ? + NADP+
-
 r
1.1.1.289 xylulose + NADPH 25.7% of the rate with L-sorbose Gluconobacter oxydans ? + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.289 monomer 1 * 60000, SDS-PAGE Gluconobacter oxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.289 30
-
-
 Gluconobacter oxydans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.289 30
-
 pH 6.5-7.5, 30 min, stable Gluconobacter oxydans
1.1.1.289 55
-
 stable up to, for 10 min Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.289 7
-
 reduction of L-sorbose Gluconobacter oxydans
1.1.1.289 10 10.5 oxidation of D-sorbitol Gluconobacter oxydans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.289 6.5 7.5 30°C, stable for 30 min Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.289 NADP+
-
 Gluconobacter oxydans
1.1.1.289 NADPH
-
 Gluconobacter oxydans