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Literature summary extracted from
- Cloning and expression of Phanerochaete chrysosporium cellobiohydrolase (cbhl.2) (2005), Afr. J. Biotechnol., 4, 1180-1184.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.91 | gene cbhl.2, genomic library construction, DNA and amino acid sequence determination and analysis, expression of inactive enzyme in Escherichia coli strain XL 1-Blue in membranes and inclusion bodies | Phanerodontia chrysosporium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.91 | Phanerodontia chrysosporium | Q7LHI2 | i.e. Sporotrichum pruinosum, strain ME446, gene cbhl.2 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.91 | 4-methylumbelliferyl beta-D-cellobioside + H2O | - |
Phanerodontia chrysosporium | 4-methylumbelliferol + beta-D-cellobiose | - |
? |  |
| 3.2.1.91 | 4-methylumbelliferyl beta-D-lactoside + H2O | - |
Phanerodontia chrysosporium | 4-methylumbelliferol + beta-D-lactose | - |
? | |
| 3.2.1.91 | 4-nitrophenyl beta-cellobioside + H2O | - |
Phanerodontia chrysosporium | 4-nitrophenol + beta-cellobiose | - |
? | |
| 3.2.1.91 | 4-nitrophenyl beta-lactoside + H2O | - |
Phanerodontia chrysosporium | 4-nitrophenol + beta-lactose | - |
? | |
| 3.2.1.91 | avicel + H2O | - |
Phanerodontia chrysosporium | ? | - |
? | |
| 3.2.1.91 | carboxymethyl cellulose + H2O | - |
Phanerodontia chrysosporium | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.91 | ? | x * 50000-60000, recombinant enzyme, SDS-PAGE | Phanerodontia chrysosporium |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.91 | Cbhl.2 | - |
Phanerodontia chrysosporium |
| 3.2.1.91 | cellobiohydrolase | - |
Phanerodontia chrysosporium |
| 3.2.1.91 | Cellulase | - |
Phanerodontia chrysosporium |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.91 | 50 | - |
assay at | Phanerodontia chrysosporium |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.91 | 5 | - |
assay at | Phanerodontia chrysosporium |
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