Literature summary extracted from
Zhang, Y.; Li, H.
Structure determination of a truncated dimeric splicing endonuclease in pseudo-face-centered space group P2(1)2(1)2 (2004), Acta Crystallogr. Sect. D, 60, 447-452.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.6.1.16 |
gene AFN, expression of truncated fully active dimeric enzyme form lacking the N-terminal domain in Escherichia coli strain BLR(DE3) |
Archaeoglobus fulgidus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.6.1.16 |
truncated active dimeric enzyme form lacking the N-terminal domain, hanging drop vapour diffusion method, 60 mg/ml protein is mixed with reservoir solution conatining 0.1 M sodium cacodylate, pH 6.5-6.8, 20 mM ammonium sulfate, and 0.3-0.4 M sodium acetate, approximately 30 days, X-ray diffraction structure determination and analysis at 2.0 A resolution |
Archaeoglobus fulgidus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
4.6.1.16 |
additional information |
construction of a truncated fully active dimeric enzyme form lacking the N-terminal domain |
Archaeoglobus fulgidus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.6.1.16 |
Archaeoglobus fulgidus |
- |
gene AFN |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.6.1.16 |
recombinant truncated fully active dimeric enzyme form lacking the N-terminal domain from Escherichia coli strain BLR(DE3) to homogeneity |
Archaeoglobus fulgidus |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
4.6.1.16 |
pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus |
active site structure, the catalytic triad is formed by His257, Lys287, and Tyr246 |
Archaeoglobus fulgidus |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.6.1.16 |
additional information |
the enzyme recognizes a unique RNA motif that consists of two three-nucleotide bulges separated by a four base-pair helix, known as the bulge-helix-bulge motif, BHB motif |
Archaeoglobus fulgidus |
? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.6.1.16 |
AF endonuclease |
- |
Archaeoglobus fulgidus |
4.6.1.16 |
AFN |
- |
Archaeoglobus fulgidus |
4.6.1.16 |
RNA-splicing endonuclease |
- |
Archaeoglobus fulgidus |
4.6.1.16 |
splicing endonuclease |
- |
Archaeoglobus fulgidus |