BRENDA - Enzyme Database

Isolation and characterization of pigeon breast muscle cytosolic 5'-nucleotidase-I (cN-I)

Tkacz-Stachowska, K.; Lechward, K.; Skladanowski, A.C.; Acta Biochim. Pol. 52, 789-796 (2005)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.5
ADP
strong, allosteric activiator
Columba livia
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
phosphate
-
Columba livia
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
0.04
-
5'-dCMP
37°C, pH 7.0, presence of 1 mM ADP
Columba livia
3.1.3.5
0.29
-
5'-AMP
37°C, pH 7.0, presence of 1 mM ADP
Columba livia
3.1.3.5
0.75
-
5'-dCMP
37°C, pH 7.0
Columba livia
3.1.3.5
1.67
-
5'-IMP
37°C, pH 7.0, presence of 1 mM ADP
Columba livia
3.1.3.5
1.85
-
5'-AMP
37°C, pH 7.0
Columba livia
3.1.3.5
2
3
5'-dAMP
37°C, pH 7.0
Columba livia
3.1.3.5
5.2
-
5'-dAMP
37°C, pH 7.0, presence of 1 mM ADP
Columba livia
3.1.3.5
10.8
-
5'-IMP
37°C, pH 7.0
Columba livia
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.5
cytosol
-
Columba livia
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.5
43000
-
x * 43000, SDS-PAGE
Columba livia
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.5
Columba livia
-
isoform cN-I
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.5
-
Columba livia
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
muscle
breast muscle
Columba livia
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.5
4.33
-
37°C, pH 7.0
Columba livia
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
5'-AMP + H2O
absence of ADP, 60% loss of activity
663466
Columba livia
adenosine + phosphate
-
-
-
?
3.1.3.5
5'-dAMP + H2O
presence of ADP, 66% of the activity with 5’-AMP
663466
Columba livia
deoxyadenosine + phosphate
-
-
-
?
3.1.3.5
5'-dCMP + H2O
presence of ADP, 16% of the activity with 5’-AMP
663466
Columba livia
deoxycytidine + phosphate
-
-
-
?
3.1.3.5
5'-dIMP + H2O
presence of ADP, 38% of the activity with 5’-AMP
663466
Columba livia
deoxyinosine + phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.5
?
x * 43000, SDS-PAGE
Columba livia
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.5
ADP
strong, allosteric activiator
Columba livia
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
phosphate
-
Columba livia
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
0.04
-
5'-dCMP
37°C, pH 7.0, presence of 1 mM ADP
Columba livia
3.1.3.5
0.29
-
5'-AMP
37°C, pH 7.0, presence of 1 mM ADP
Columba livia
3.1.3.5
0.75
-
5'-dCMP
37°C, pH 7.0
Columba livia
3.1.3.5
1.67
-
5'-IMP
37°C, pH 7.0, presence of 1 mM ADP
Columba livia
3.1.3.5
1.85
-
5'-AMP
37°C, pH 7.0
Columba livia
3.1.3.5
2
3
5'-dAMP
37°C, pH 7.0
Columba livia
3.1.3.5
5.2
-
5'-dAMP
37°C, pH 7.0, presence of 1 mM ADP
Columba livia
3.1.3.5
10.8
-
5'-IMP
37°C, pH 7.0
Columba livia
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.5
cytosol
-
Columba livia
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.5
43000
-
x * 43000, SDS-PAGE
Columba livia
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.5
-
Columba livia
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
muscle
breast muscle
Columba livia
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.5
4.33
-
37°C, pH 7.0
Columba livia
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
5'-AMP + H2O
absence of ADP, 60% loss of activity
663466
Columba livia
adenosine + phosphate
-
-
-
?
3.1.3.5
5'-dAMP + H2O
presence of ADP, 66% of the activity with 5’-AMP
663466
Columba livia
deoxyadenosine + phosphate
-
-
-
?
3.1.3.5
5'-dCMP + H2O
presence of ADP, 16% of the activity with 5’-AMP
663466
Columba livia
deoxycytidine + phosphate
-
-
-
?
3.1.3.5
5'-dIMP + H2O
presence of ADP, 38% of the activity with 5’-AMP
663466
Columba livia
deoxyinosine + phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.5
?
x * 43000, SDS-PAGE
Columba livia