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Literature summary extracted from

  • di Salvo, M.L.; Hunt, S.; Schirch, V.
    Expression, purification, and kinetic constants for human and Escherichia coli pyridoxal kinases (2004), Protein Expr. Purif., 36, 300-306.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.35 expressed in Escherichia coli HMS174(DE3) Homo sapiens
2.7.1.35 kinase 1 and 2 expressed in Escherichia coli HMS174(DE3), kinase 2 additionally expressed as His-tag fusion protein Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.35 0.01
-
pyridoxamine kinase 1 with ZnATP2- as substrate, pH 7.3, 37°C Escherichia coli
2.7.1.35 0.015
-
pyridoxine with ZnATP2- as substrate, pH 7.3, 37°C Homo sapiens
2.7.1.35 0.02
-
pyridoxine with MgATP2- as substrate, pH 7.3, 37°C Homo sapiens
2.7.1.35 0.025
-
pyridoxine kinase 1 with MgATP2- as substrate, pH 7.3, 37°C Escherichia coli
2.7.1.35 0.03
-
pyridoxamine kinase 1 with MgATP2- as substrate, pH 7.3, 37°C Escherichia coli
2.7.1.35 0.03
-
pyridoxal with MgATP2- as substrate, pH 7.3, 37°C Homo sapiens
2.7.1.35 0.035
-
pyridoxamine with MgATP2- as substrate, pH 7.3, 37°C Homo sapiens
2.7.1.35 0.07
-
ATP kinase 1 with pyridoxal as substrate, pH 7.3, 37°C Escherichia coli
2.7.1.35 0.07
-
ATP ATP in form of ZnATP2- Escherichia coli
2.7.1.35 0.1
-
pyridoxal kinase 1 with MgATP2- as substrate, pH 7.3, 37°C Escherichia coli
2.7.1.35 0.19
-
pyridoxal kinase 1 with ZnATP2- as substrate, pH 7.3, 37°C Escherichia coli
2.7.1.35 0.35
-
pyridoxal with ZnATP2- as substrate, pH 7.3, 37°C Homo sapiens
2.7.1.35 0.42
-
ATP with pyridoxal as substrate, pH 7.3, 37°C Homo sapiens
2.7.1.35 0.42
-
ATP ATP in form of MgATP Homo sapiens
2.7.1.35 0.6
-
ATP kinase 1 with pyridoxal as substrate, pH 7.3, 37°C Escherichia coli
2.7.1.35 0.6
-
ATP ATP in form of MgATP2- Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.35 Mg2+ in complex with ATP most likely preferred substrate in vivo Escherichia coli
2.7.1.35 Mg2+ in complex with ATP most likely preferred substrate in vivo Homo sapiens
2.7.1.35 Zn2+ in complex with ATP preferred substrate in vitro Escherichia coli
2.7.1.35 Zn2+ in complex with ATP preferred substrate in vitro Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.35 ATP + pyridoxal Escherichia coli generates the active form of vitamin B6, which serves as cofactor for many enzymes ADP + pyridoxal 5'-phosphate
-
?
2.7.1.35 ATP + pyridoxal Homo sapiens generates the active form of vitamin B6, which serves as cofactor for many enzymes ADP + pyridoxal 5'-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.35 Escherichia coli
-
kinase 1
-
2.7.1.35 Homo sapiens O00764
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.35
-
Escherichia coli
2.7.1.35
-
Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.35 0.052
-
purified kinase 2 enzyme, pH 6.1, 37°C Escherichia coli
2.7.1.35 2.5
-
purified enzyme, pH 6.1, 37°C Homo sapiens
2.7.1.35 2.7
-
purified kinase 1 enzyme, pH 6.1, 37°C Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.1.35 -80°C, 20 mM phosphate buffer, pH 7.3, stable for at least some weeks Escherichia coli
2.7.1.35 -80°C, 20 mM phosphate buffer, pH 7.3, stable for at least some weeks Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.35 ATP + pyridoxal
-
Escherichia coli ADP + pyridoxal 5'-phosphate
-
?
2.7.1.35 ATP + pyridoxal
-
Homo sapiens ADP + pyridoxal 5'-phosphate
-
?
2.7.1.35 ATP + pyridoxal generates the active form of vitamin B6, which serves as cofactor for many enzymes Escherichia coli ADP + pyridoxal 5'-phosphate
-
?
2.7.1.35 ATP + pyridoxal generates the active form of vitamin B6, which serves as cofactor for many enzymes Homo sapiens ADP + pyridoxal 5'-phosphate
-
?
2.7.1.35 ATP + pyridoxamine
-
Escherichia coli ADP + pyridoxamine 5'-phosphate
-
?
2.7.1.35 ATP + pyridoxamine
-
Homo sapiens ADP + pyridoxamine 5'-phosphate
-
?
2.7.1.35 ATP + pyridoxine
-
Escherichia coli ADP + pyridoxine 5'-phosphate
-
?
2.7.1.35 ATP + pyridoxine
-
Homo sapiens ADP + pyridoxine 5'-phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.35 ePL kinase two different forms encoded by genes PdxK and PdxY yielding kinase 1 and kinase 2, low activity of kinase 2 suggests that this kinase originally has another substrate but can also utilize pyridoxal Escherichia coli
2.7.1.35 hPL kinase
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.35 0.1
-
pyridoxine with ZnATP2- as substrate, pH 7.3, 37°C Homo sapiens
2.7.1.35 0.3
-
pyridoxine kinase 1 with MgATP2- as substrate, pH 7.3, 37°C Escherichia coli
2.7.1.35 0.3
-
pyridoxine with MgATP2- as substrate, pH 7.3, 37°C Homo sapiens
2.7.1.35 0.3
-
pyridoxamine with MgATP2- as substrate, pH 7.3, 37°C Homo sapiens
2.7.1.35 0.4
-
pyridoxamine kinase 1 with ZnATP2- as substrate, pH 7.3, 37°C Escherichia coli
2.7.1.35 0.5
-
pyridoxal with ZnATP2- as substrate, pH 7.3, 37°C Homo sapiens
2.7.1.35 0.7
-
pyridoxamine kinase 1 with MgATP2- as substrate, pH 7.3, 37°C Escherichia coli
2.7.1.35 0.8
-
pyridoxal with MgATP2- as substrate, pH 7.3, 37°C Homo sapiens
2.7.1.35 2
-
pyridoxal kinase 1 with ZnATP2- as substrate, pH 7.3, 37°C Escherichia coli
2.7.1.35 2.3
-
pyridoxal kinase 1 with MgATP2- as substrate, pH 7.3, 37°C Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.35 Escherichia coli kinase 1, calculated from the deduced amino acid sequence
-
5.14
2.7.1.35 Homo sapiens calculated from the deduced amino acid sequence
-
5.75
2.7.1.35 Escherichia coli kinase 2, calculated from the deduced amino acid sequence
-
6.05